A5052348100- Ubiquitin and proteasome pathways
- Histone Deacetylase Inhibitors Research
- Retinoids in leukemia and cellular processes
- Protein Degradation and Inhibitors
- Acute Myeloid Leukemia Research
- Peptidase Inhibition and Analysis
- Genetics and Neurodevelopmental Disorders
- Acute Lymphoblastic Leukemia research
- Chronic Myeloid Leukemia Treatments
- Microtubule and mitosis dynamics
- interferon and immune responses
- Chronic Lymphocytic Leukemia Research
- Cancer-related Molecular Pathways
- RNA modifications and cancer
- Multiple Myeloma Research and Treatments
- Natural product bioactivities and synthesis
- Cancer-related gene regulation
- NF-κB Signaling Pathways
- Heat shock proteins research
- Autophagy in Disease and Therapy
- Cancer, Hypoxia, and Metabolism
- Cell Adhesion Molecules Research
- Genomics, phytochemicals, and oxidative stress
- Viral-associated cancers and disorders
- Ferroptosis and cancer prognosis
Shanghai Jiao Tong University
2016-2025
Tongren Hospital
2016-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2022
The First Affiliated Hospital, Sun Yat-sen University
2017
Sun Yat-sen University
2017
Ministry of Education
2010
Abstract Identifying novel drug targets to overcome resistance tyrosine kinase inhibitors (TKIs) and eradicating leukemia stem/progenitor cells are required for the treatment of chronic myelogenous (CML). Here, we show that ubiquitin-specific peptidase 47 (USP47) is a potential target TKI resistance. Functional analysis shows USP47 knockdown represses proliferation CML sensitive or resistant imatinib in vitro vivo. The knockout Usp47 significantly inhibits BCR-ABL T315I -induced mice with...
SUMO-specific protease 1 (SENP1), a member of the de-SUMOylation family, is elevated in prostate cancer (PCa) cells and involved PCa pathogenesis. Momordin Ιc (Mc), natural pentacyclic triterpenoid, inhibited SENP1 vitro, as reflected by reduced SENP1C-induced cleavage SUMO2-ΔRanGAP1. Mc also altered thermal stability newly developed cellular shift assay, indicating that directly interacts with cells. Consistent inhibition, increased SUMOylated protein levels, which was further confirmed...
Sentrin/SUMO (small ubiquitin-like modifier)-specific proteases (SENPs) have been implicated in the development of prostate cancer. However, due to low abundance SUMO-modified proteins and high activity SENPs, SUMO substrates affected by SENPs cancer cells are largely unknown. Here, we identified SI2, a novel cell-permeable SENP-specific inhibitor, high-throughput screening. Using SI2 as way inhibiting stably transfected PC3 model, combination with stable isotope labeling amino acids (SILAC)...
// Dongjun Qin 1, * , Weiwei Wang Hu Lei Hao Luo Haiyan Cai 1 Caixia Tang Yunzhao Wu Yingying 2 Jin Weilie Xiao 3 Tongdan Chunmin Ma Hanzhang Xu Jinfu Zhang Fenghou Gao Ying-Li Hongqiao International Institute of Medicine, Shanghai Tongren Hospital/Faculty Basic Chemical Biology Division Universities E-Institutes, Key Laboratory Cell Differentiation and Apoptosis the Chinese Ministry Education, Jiao Tong University School Shanghai, China Oncology, 9th People's Hospital, State Phytochemistry...
Aberrant alternative splicing is one of the hallmarks cancer and potentially based on upregulated expression factors in some types cancer. Our previous study suggested that factor RBM39 significantly multiple myeloma (MM) its upregulation positively associated with poor prognosis. Here, we further demonstrate survival proliferation MM cells rely knockdown inhibits malignant growth MM. Indisulam, a "molecular glue" mediates proteasomal degradation RBM39, has potent suppressive effects both...
T-cell acute lymphoblastic leukemia (T-ALL) is a highly aggressive that primarily caused by aberrant activation of the NOTCH1 signaling pathway. Recent studies have revealed posttranslational modifications, such as ubiquitination, regulate stability, activity, and localization. However, specific deubiquitinase affects protein stability remains unestablished. Here, we report ubiquitin-specific protease 7 (USP7) can stabilize NOTCH1. USP7 deubiquitinated in vivo vitro, whereas knockdown...
The normal epithelial cell-specific-1 (NES1) gene, also named as KLK10, is recognised a novel putative tumour suppressor in breast cancer, but few studies have focused on the function of KLK10 human prostate cancer. Our study confirms that expression cancer tissue and cell lines (PC3, DU145, LNCaP clone FGC) low. Given androgen-independent growth characteristic PC3 line more similar to clinical castration-resistant we studied role PC3. In vitro vivo assays showed over-expressing could...
FBOX6 ubiquitin ligase complex is involved in the endoplasmic reticulum-associated degradation pathway by mediating ubiquitination of glycoproteins. FBXO6 interacts with chitobiose unfolded N-glycoprotein, pointing glycoproteins toward E2 for ubiquitination. Although glycoprotein-recognizing mechanism well documented, its bona fide interacting are largely unknown. Here we utilized a protein purification approach combined LC-MS to systematically identify FBXO6-interacting Following...
Abstract Despite recent progress in the treatment, outcome of adult acute T-cell lymphoblastic leukemia (T-ALL) is poor. Development novel approach to combat this disease urgently required. Vorinostat, a pan-histone deacetylase (HDAC) inhibitor, exerts promising anticancer activity variety solid and hematologic malignancies. However, efficacy vorinostat monotherapy unsatisfactory. Here, we show that quinacrine (QC), an anti-malaria drug with potent autophagy inhibitory activity, could...
Abstract Aberrant upregulation of the ubiquitin-specific protease 14 (USP14) has been found in some malignant tumors, including oral squamous cell carcinoma (OSCC). In this study, we further demonstrated that aberrantly overexpressed USP14 was also closely related to adverse clinicopathological features and poor prognosis patients with OSCC, so hypothesized might act as a tumor-promoting factor during progression OSCC. Notably, originally proved is deubiquitinating enzyme for...
KIF18A, a molecular motor, is an essential component in the regulation of orderly chromosome congression by attenuation kinetochore oscillation amplitude at midzone during mitosis vertebrate cells. Here we report that KIF18A depletion resulted mitotic arrest which was accompanied presence unaligned chromosomes HeLa This resembles phenotype induced impaired function CENP-E, also kinesin for formation spindles. Our further analysis showed caused specific down-regulation CENP-E. Down-regulation...
In this study, we report the functional characterization of a new ent-kaurene diterpenoid termed pharicin A, which was originally isolated from Isodon xerophilus, perennial shrub frequently used in Chinese folk medicine for tumor treatment. Pharicin A induces mitotic arrest leukemia and solid tumor-derived cells identified by their morphology, DNA content, marker analyses. A-induced is associated with unaligned chromosomes, aberrant BubR1 localization, deregulated spindle checkpoint...
T-cell acute lymphoblastic leukemia (T-ALL) is a lymphoid malignancy caused by the oncogenic transformation of immature progenitors with poor outcomes. WP1130 has shown potent activity against variety cancer but whether anti-T-ALL not clear. USP24, one target WP1130, largest deubiquitinases and its detailed mechanism poorly understood. The aim this study was to explore could suppress T-ALL role USP24 in T-ALL.Molecular docking cellular thermal shift assay were performed determine how...
Synthetic triterpenoid methyl-2-cyano-3, 12-dioxooleana-1, 9(11)-dien-28-oate (CDDO-Me) has been shown as a promising agent against ovarian cancer. However, the underlying mechanism is not well understood. Here, we demonstrate that CDDO-Me directly interacts with Hsp90 in cells by cellular thermal shift assay. treatment leads to upregulation of Hsp70 and degradation clients (ErbB2 Akt), indicating inhibition cells. Knockdown significantly inhibits cell proliferation enhances...
Psoriatic keratinocytes express exaggerated levels of inflammatory cytokines, and show aberrant hyperproliferation terminal differentiation in the pathogenesis psoriasis. The antimicrobial protein hS100A7 (psoriasin) has been found highly expressed psoriatic skin, but mechanism physiological function remain largely unknown. We observed that induces mature interleukin 1α (17kDa) expression normal human epidermal keratinocytes, which is dependent on RAGE-p38 MAPK calpain-1 as inhibitors or...