A5015421996- Glycosylation and Glycoproteins Research
- SARS-CoV-2 and COVID-19 Research
- Computational Drug Discovery Methods
- Photoreceptor and optogenetics research
- Carbohydrate Chemistry and Synthesis
- Synthesis and biological activity
- Lipid Membrane Structure and Behavior
- Mass Spectrometry Techniques and Applications
- Bacteriophages and microbial interactions
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Fungal Biology and Applications
- Synthesis and Characterization of Heterocyclic Compounds
- Monoclonal and Polyclonal Antibodies Research
- Click Chemistry and Applications
- Enzyme Structure and Function
- Protist diversity and phylogeny
- Marine Toxins and Detection Methods
- Ubiquitin and proteasome pathways
- Biochemical and Molecular Research
- Cellular transport and secretion
- Enzyme Production and Characterization
- Photosynthetic Processes and Mechanisms
- Physiological and biochemical adaptations
- Biochemical and Structural Characterization
Institute of Biological Chemistry, Academia Sinica
2005-2025
Academia Sinica
2005-2015
Springer Nature (Germany)
2014
National Taiwan University
2005
Most membrane proteins are modified by covalent addition of complex sugars through N- and O-glycosylation. Unlike proteins, glycans do not typically adopt specific secondary structures remain very mobile, shielding potentially large fractions protein surface. High glycan conformational freedom hinders complete structural elucidation glycoproteins. Computer simulations may be used to model glycosylated but require hundreds thousands computing hours on supercomputers, thus limiting routine...
Human coronavirus 229E (HCoV-229E) is the earliest CoV found to infect humans. It binds human aminopeptidase N (hAPN) through receptor binding domain (RBD) of its spike (S) protein achieve host recognition. We present cryo-electron microscopy structure two HCoV-229E S in complex with a dimeric hAPN provide structural insights on how opens up RBD engage receptor, information that currently missing among alphacoronaviruses which belong. quantitatively profile glycosylation and deduce...
Five active metal-conjugated inhibitors (PMA, TDT, EPDTC, JMF1586 and JMF1600) bound with the 3C-like protease of severe acute respiratory syndrome (SARS)-associated coronavirus were analyzed crystallographically. The complex structures reveal two major inhibition modes: Hg(2+)-PMA is coordinated to C(44), M(49) Y(54) a square planar geometry at S3 pocket, whereas each Zn(2+) four zinc-inhibitors tetrahedrally H(41)-C(145) catalytic dyad. For anti-SARS drug design, this Zn(2+)-centered...
Proteins can spontaneously tie a variety of intricate topological knots through twisting and threading the polypeptide chains. Recently developed artificial intelligence algorithms have predicted several new classes knotted proteins, but predictions remain to be authenticated experimentally. Here, we showed by X-ray crystallography solution state NMR spectroscopy that Q9PR55, an 89-residue protein from Ureaplasma urealyticum, possesses novel 71 topology is accurately AlphaFold 2, except for...
The ongoing evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), resulting in the emergence new variants that are resistant to existing vaccines and therapeutic antibodies, has raised need for novel strategies combat persistent global COVID-19 epidemic. In this study, a monoclonal anti-human angiotensin-converting enzyme (hACE2) antibody, ch2H2, was isolated humanized block viral receptor-binding domain (RBD) binding hACE2, major entry receptor SARS-CoV-2. This antibody...
In a successful example of lead optimization by computer modeling prediction, computational technology was used to optimize inhibitor (TL-3) the SARS-CoV 3CL protease. A novel C2-symmetric diol (1) then designed and synthesized, displayed higher affinity than original compound one order magnitude in its inhibition constant (0.6→0.073 μM). We believe that this approach has provided platform for further optimization. Supporting information article is available on WWW under...
Since its emergence, SARS-CoV-2 has been continuously evolving, hampering the effectiveness of current vaccines against COVID-19. mAbs can be used to treat patients at risk severe Thus, development broadly protective and an understanding underlying mechanisms are great importance. Here, we isolated from donors with breakthrough infection Omicron subvariants using a single-B cell screening platform. We identified mAb, O5C2, which possesses broad-spectrum neutralization antibody-dependent...
Kinetic measurement of protein folding is limited by the method used to trigger folding. Traditional methods, such as stopped flow, have a long mixing dead time and cannot be monitor fast processes. Here, we report compound, 4-(bromomethyl)-6,7-dimethoxycoumarin, that can "photolabile cage" study early stages The process protein, RD1, including kinetics, enthalpy, volume change, was studied combined use phototriggered caging strategy time-resolved photoacoustic calorimetry. cage caused...
Abstract In silico algorithms have been the common approach for transmembrane (TM) protein topology prediction. However, computational tools may produce questionable results and experimental validation has proven difficult. Although biochemical strategies are available to determine C-terminal orientation of TM proteins, N-terminal still limited but needed because end is essential membrane targeting. Here, we describe a new easy method effectively target proteins in Escherichia coli plasma...
Specific antibody interactions with short peptides have made epitope tagging systems a vital tool employed in virtually all fields of biological research. Here, we present novel system comprised monoclonal named GD‐26, which recognises the TD peptide (GTGATPADD) derived from Haloarcula marismortui bacteriorhodopsin I ( Hm BRI) D94N mutant. The crystal structure antigen‐binding fragment (Fab) GD‐26 complexed was determined to resolution 1.45 Å. found adopt 3 10 helix conformation within...