A5019463541- Plant biochemistry and biosynthesis
- Computational Drug Discovery Methods
- Enzyme Structure and Function
- SARS-CoV-2 and COVID-19 Research
- Biochemical and Molecular Research
- Microbial Natural Products and Biosynthesis
- ATP Synthase and ATPases Research
- Biochemical Acid Research Studies
- Synthesis and biological activity
- Natural product bioactivities and synthesis
- Heat shock proteins research
- Nitrogen and Sulfur Effects on Brassica
- Biofuel production and bioconversion
- Chemical Reaction Mechanisms
- Enzyme Catalysis and Immobilization
- RNA and protein synthesis mechanisms
- Viral Infections and Immunology Research
- Enzyme Production and Characterization
- Protein Structure and Dynamics
- Viral gastroenteritis research and epidemiology
- Peptidase Inhibition and Analysis
- Microbial Metabolic Engineering and Bioproduction
- HIV/AIDS drug development and treatment
- interferon and immune responses
- Metal complexes synthesis and properties
Institute of Biological Chemistry, Academia Sinica
2014-2024
Academia Sinica
2007-2024
National Taiwan University
2014-2024
National Sun Yat-sen University
2016
National Chung Hsing University
2015
Institute of Molecular Biology, Academia Sinica
2015
Weatherford College
2013
Affiliated Hospital of Hangzhou Normal University
2012
Korea Institute of Science & Technology Information
2010
National Yang Ming Chiao Tung University
2004-2009
In this study, 221 phytocompounds were evaluated for activity against anti-severe acute respiratory syndrome associated coronavirus (SARS-CoV) activities using a cell-based assay measuring SARS-CoV-induced cytopathogenic effect on Vero E6 cells. Ten diterpenoids (1-10), two sesquiterpenoids (11 and 12), triterpenoids (13 14), five lignoids (15-19), curcumin (20), reference controls niclosamide (21) valinomycin (22) potent inhibitors at concentrations between 3.3 10 microM. The of the 22...
Severe acute respiratory syndrome (SARS) is an infectious disease caused by a novel human coronavirus. Currently, no effective antiviral agents exist against this type of virus. A cell-based assay, with SARS virus and Vero E6 cells, was developed to screen existing drugs, natural products, synthetic compounds identify anti-SARS agents. Of >10,000 tested, approximately 50 were found active at 10 microM; among these compounds, two are drugs (Reserpine 13 Aescin 5) several in clinical...
SARS‐CoV is the causative agent of severe acute respiratory syndrome (SARS). The virally encoded 3C‐like protease (3CLPro) has been presumed critical for viral replication in infected host cells. In this study, we screened a natural product library consisting 720 compounds inhibitory activity against 3CLPro. Two were found to be inhibitive: tannic acid (IC50 = 3 µM) and 3‐isotheaflavin‐3‐gallate (TF2B) 7 µM). These two belong group polyphenols tea. We further investigated 3CLPro‐inhibitory...
Caspases have been strongly implicated to play an essential role in apoptosis. A critical question regarding the role(s) of these proteases is whether selective inhibition effector caspase(s) will prevent cell death. We identified potent and non-peptide inhibitors caspases 3 7. The apoptosis maintenance functionality with a caspase 3/7-selective inhibitor demonstrated for first time, suggests that targeting two alone sufficient blocking Furthermore, x-ray co-crystal structure complex between...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAncestry of the 4-chlorobenzoate dehalogenase: analysis amino acid sequence identities among families acyl:adenyl ligases, enoyl-CoA hydratases/isomerases, and acyl-CoA thioesterasesPatricia C. Babbitt, George L. Kenyon, Brian M. Martin, Hugues Charest, Michel Slyvestre, Jeffrey D. Scholten, Kai Hsuan Chang, Po Huang Liang, Debra Dunaway-MarianoCite this: Biochemistry 1992, 31, 24, 5594–5604Publication Date (Print):June 1, 1992Publication History...
Bisphosphonate drugs (e.g., Fosamax and Zometa) are thought to act primarily by inhibiting farnesyl diphosphate synthase (FPPS), resulting in decreased prenylation of small GTPases. Here, we show that some bisphosphonates can also inhibit geranylgeranyl (GGPPS), as well undecaprenyl (UPPS), a cis-prenyltransferase interest target for antibacterial therapy. Our results on GGPPS (10 structures) there three bisphosphonate-binding sites, consisting FPP or isopentenyl substrate-binding sites...
Severe acute respiratory syndrome coronavirus (SARS-CoV) main protease (Mpro), a protein required for the maturation of SARS-CoV, is vital its life cycle, making it an attractive target structure-based drug design anti-SARS drugs. The virtual screening chemical database containing 58 855 compounds followed by testing potential SARS-CoV Mpro inhibition leads to two hit compounds. core structures these hits, defined docking study, are used further analogue search. Twenty-one analogues derived...
Development of anti-severe acute respiratory syndrome associated coronavirus (SARS-CoV) agents is pivotal to prevent the reemergence life-threatening disease, SARS. In this study, more than 200 extracts from Chinese medicinal herbs were evaluated for anti-SARS-CoV activities using a cell-based assay that measured SARS-CoV-induced cytopathogenic effect (CPE) in vitro on Vero E6 cells. Six herbal extracts, one each Gentianae Radix (龍膽 lóng dǎn; dried rhizome Gentiana scabra), Dioscoreae...
Human coxsackievirus (CV) belongs to the picornavirus family, which consists of over 200 medically relevant viruses. In picornavirus, a chymotrypsin-like protease (3C(pro)) is required for viral replication by processing polyproteins, and thus it regarded as an antiviral drug target. A 3C-like (3CL(pro)) also exists in human coronaviruses (CoV) such 229E one causing severe acute respiratory syndrome (SARS). To combat SARS, we previously had developed peptidomimetic zinc-coordinating...
The outbreak of coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome 2 (SARS-CoV-2), is a global threat to human health. Using multidisciplinary approach, we identified and validated hepatitis C virus (HCV) protease inhibitor simeprevir as an especially promising repurposable drug for treating COVID-19. Simeprevir potently reduces SARS-CoV-2 viral load multiple orders magnitude synergizes with remdesivir in vitro. Mechanistically, showed that not only inhibits...
Undecaprenyl pyrophosphate synthase (UPPs) catalyzes the consecutive condensation reactions of a farnesyl (FPP) with eight isopentenyl pyrophosphates (IPP), in which new cis-double bonds are formed, to generate undecaprenyl that serves as lipid carrier for peptidoglycan synthesis bacterial cell wall. The structures Escherichia coli UPPs were determined previously an orthorhombic crystal form apoenzyme, complex Mg(2+)/sulfate/Triton, and bound FPP. In further search its catalytic mechanism,...
Terpenes (isoprenoids), derived from isoprenyl pyrophosphates, are versatile natural compounds that act as metabolism mediators, plant volatiles, and ecological communicators. Divergent evolution of homomeric prenyltransferases (PTSs) has allowed PTSs to optimize their active-site pockets achieve catalytic fidelity diversity. Little is known about heteromeric PTSs, particularly the mechanisms regulating formation specific products. Here, we report crystal structure (LSU . SSU)(2)-type...
β-Glucosidase (EC 3.2.1.21) plays an essential role in biofuel production since it can cleave β-1,4-glycosidic bond to convert cellobiose into fermentable glucose. Based on the structure of Trichoderma reesei β-glucosidase 2 (TrBgl2) we solved, amino acids outer channel active site were mutated this study. Mutants P172L and P172L/F250A showed most enhanced kcat/Km kcat values by 5.3- 6.9-fold, respectively, compared wild type (WT) toward 4-nitrophenyl-β-d-glucopyranoside (p-NPG) substrate at...
Coronavirus (CoV) disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has claimed many lives worldwide and is still spreading since December 2019. The 3C-like protease (3CL pro ) papain-like (PL are essential for maturation of viral polyproteins in SARS-CoV-2 life cycle thus regarded as key drug targets disease.