A5034255384- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Immune Cell Function and Interaction
- Animal Virus Infections Studies
- Tryptophan and brain disorders
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 detection and testing
- interferon and immune responses
- Viral Infections and Outbreaks Research
- HIV Research and Treatment
- Neuroinflammation and Neurodegeneration Mechanisms
- MicroRNA in disease regulation
- Phagocytosis and Immune Regulation
- Immune Response and Inflammation
- Adenosine and Purinergic Signaling
- Inflammasome and immune disorders
- Influenza Virus Research Studies
- Long-Term Effects of COVID-19
- Child Nutrition and Feeding Issues
- COVID-19 diagnosis using AI
- RNA Interference and Gene Delivery
- Computational Drug Discovery Methods
- Diet and metabolism studies
- Clinical Nutrition and Gastroenterology
- Cytokine Signaling Pathways and Interactions
National Taiwan University
2020-2023
En Chu Kong Hospital
2022
Buddhist Tzu Chi General Hospital
2011-2012
A major challenge to end the pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is develop a broadly protective vaccine that elicits long-term immunity. As key immunogen, viral surface spike (S) protein frequently mutated, and conserved epitopes are shielded glycans. Here, we revealed S glycosylation has site-differential effects on infectivity. We found generated lung epithelial cells glycoforms associated with increased Compared fully glycosylated protein,...
The emergence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants has altered the trajectory COVID-19 pandemic and raised some uncertainty on long-term efficiency vaccine strategy. development new therapeutics against a wide range SARS-CoV-2 is imperative. We, here, have designed an inhalable siRNA, C6G25S, which covers 99.8% current capable inhibiting dominant strains, including Alpha, Delta, Gamma, Epsilon, at picomolar ranges IC50 in vitro. Moreover, C6G25S could...
Coronavirus (CoV) disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has claimed many lives worldwide and is still spreading since December 2019. The 3C-like protease (3CL pro ) papain-like (PL are essential for maturation of viral polyproteins in SARS-CoV-2 life cycle thus regarded as key drug targets disease.
Most of SARS-CoV-2 neutralizing antibodies (nAbs) targeted the receptor binding domain (RBD) spike (S) protein. However, mutations at RBD sequences found in emerging variants greatly reduced effectiveness nAbs. Here we showed that four nAbs, S2-4D, S2-5D, S2-8D, and S2-4A, which recognized a conserved epitope S2 subunit S protein, can inhibit infection through blocking protein-mediated membrane fusion. Notably, these nAbs exhibited broadly activity against Alpha, Gamma, Delta, Epsilon...
Antipsychotic drugs (APDs) are widely used to alleviate a number of psychic disorders and may have immunomodulatory effects. However, the previous studies cytokine immune regulation in APDs quite inconsistent. The aim this study was examine vitro effects different ADPs on production by peripheral blood mononuclear cells (PBMCs). We examined risperidone, clozapine, haloperidol phorbol myristate acetate ionomycin-induced interferon-γ (IFN-γ)/interleukin (IL)-4 PBMCs using intracellular...
Abstract The recent emerging coronavirus, SARS-CoV-2, has been rapidly and widely spread causing an ongoing viral pneumonia outbreak worldwide. It observed that SARS-CoV-2 patients show a rather long asymptomatic incubation time. We characterized the abilities to induce response IFNβ/IFNλ1 of two or our clinical isolates, SARS-CoV-2/NTU01/TWN/human/2020 SARS-CoV-2/NTU02/TWN/human/2020, which exhibit only amino acid differences over ∼30kb genome. found both isolates may infect Huh7, A549...
Recombinant proteins are essential in the development of subunit vaccines. In design many recombinant proteins, polyhistidine residues added to N- or C-termini target sequences facilitate purification. However, whether addition tag influences immunogenicity remains unknown. this study, tag-free SARS-CoV-2 RBD and His-tag were investigated determine there any differences their receptor binding affinity immunogenicity. The results showed that protein had a higher for with hACE2 receptors than...
Enterobacterales clinical isolates are now being resistant to clinically achievable concentrations of most commonly used antibiotics that makes treatment hospitalized patients very challenging. We hereby determine the molecular characteristics carbapenemase genes in carbapenem-resistant (CRE) Taiwan. A total 455 CRE were identified between August 2011 July 2020. Minimum inhibitory for selected carbapenems tested using Vitek 2, and determined polymerase chain reaction combination with...
Development of specific antivirals is an urgent unmet need for SARS-coronavirus 2 (SARS-CoV-2) infections. This study focuses on host proteases that proteolytically activate the SARS-CoV-2 spike protein, critical its fusion activity after binding to ACE2 receptor, as antiviral targets. We first validated cleavage at a putative furin substrate motif by expressing it in VeroE6 cells and found prominent syncytium formation. Both were abolished treatment with inhibitors...
Type I interferons are important antiviral cytokines, but prolonged interferon production is detrimental to the host. The TLR3-driven immune response crucial for mammalian immunity, and its intracellular localization determines induction of type interferons; however, mechanism terminating TLR3 signaling remains obscure. Here, we show that E3 ubiquitin ligase ZNRF1 controls sorting into multivesicular bodies/lysosomes terminate production. Mechanistically, c-Src kinase activated by engagement...
Patients recovering from COVID-19 may need vaccination against SARS-CoV-2 because acquired immunity primary infection wane, given the emergence of new variants. Understanding trends anti-spike IgG and neutralizing antibody titers in patients inform decision made on appropriate interval between recovery vaccination. Participants aged 20 years or older diagnosed with January December, 2020 were enrolled. Serum specimens collected every three months 10 days to 12 after onset symptom for...
Abstract A major challenge to end the pandemic caused by SARS-CoV-2 is develop a broadly protective vaccine. As key immunogen, spike protein frequently mutated with conserved epitopes shielded glycans. Here, we reveal that glycosylation has site-differential effects on viral infectivity and lung epithelial cells generate more infective glycoforms. Compared fully glycosylated spike, immunization of N-glycans trimmed monoglycosylated state (S mg ) elicits stronger immune responses better...
Although real-time reverse transcriptase polymerase chain reaction (real-time RT-PCR) remains as a golden standard for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, it can not be easily expanded to large-scaled screening during outbreaks, and the positive results do necessarily correlate with infectious status identified subjects. In this study, performance Vstrip® RV2 COVID-19 Antigen Rapid Test (RAT) its correlation virus infectivity was examined by...
ABSTRACT Since the D614G substitution in spike (S) of SARS-CoV-2 emerged, variant strain underwent rapid expansion to become most abundant worldwide. Therefore, this may provide an advantage viral spreading. To explore mechanism, we analyzed 18 isolates containing S proteins with either G614 or D614. Both virus titer and syncytial phenotype were significantly increased S-G614 than S-D614 isolates. We further showed cleavage at furin substrate site, a key event that promotes syncytium, These...
Abstract An increasing body of evidence emphasizes the role metabolic reprogramming in immune cells to fight off infections. However, little is known about regulation metabolite transporters that facilitate and support demands. In this study, we found equilibrative nucleoside transporter 3 (ENT3) expression part innate response, rapidly upregulated upon bacterial viral infection. The transcription ENT3 directly under IFN-induced signaling, positioning as an Interferon-stimulated gene (ISG)....
We reported a 72 y/o male patient who suffered from esophageal obstruction due to carcinoma. Percutaneous endoscopic gastrostomy was performed after dilatation. However pulled out the tube accidentally 38 hours this procedure, rupture of with internal bumper retained in gastric lumen occurred. After replacement another by method, did well without episode peritonitis and survived for 4 months finally. Reviewing cases literature, should be firstly considered case immediate extrusion...