A5091173337- Semiconductor materials and devices
- Advancements in Semiconductor Devices and Circuit Design
- Semiconductor Quantum Structures and Devices
- Glycosylation and Glycoproteins Research
- Advanced Memory and Neural Computing
- Electronic and Structural Properties of Oxides
- Carbohydrate Chemistry and Synthesis
- Nanowire Synthesis and Applications
- X-ray Diffraction in Crystallography
- Viral gastroenteritis research and epidemiology
- Phase-change materials and chalcogenides
- Semiconductor materials and interfaces
- RNA and protein synthesis mechanisms
- Crystallization and Solubility Studies
- SARS-CoV-2 and COVID-19 Research
- Protein Structure and Dynamics
- Chemical Synthesis and Analysis
- Genomics and Phylogenetic Studies
- ZnO doping and properties
- Magnetic Bearings and Levitation Dynamics
- Microfluidic and Capillary Electrophoresis Applications
- vaccines and immunoinformatics approaches
- GaN-based semiconductor devices and materials
- Electric Motor Design and Analysis
- Machine Learning in Bioinformatics
Kaohsiung Medical University
2021-2023
Kaohsiung Medical University Chung-Ho Memorial Hospital
2023
Genomics Research Center, Academia Sinica
2019-2022
IBM (United States)
2013-2022
National Cheng Kung University
2010-2021
Tainan University of Technology
2021
Alliance for Safe Kids
2021
IBM Research - Thomas J. Watson Research Center
2014-2020
People's Liberation Army 411 Hospital
2020
Academia Sinica
2012-2018
The outbreak of COVID-19 caused by SARS-CoV-2 has resulted in more than 50 million confirmed cases and over 1 deaths worldwide as November 2020. Currently, there are no effective antivirals approved the Food Drug Administration to contain this pandemic except antiviral agent remdesivir. In addition, trimeric spike protein on viral surface is highly glycosylated almost 200,000 variants with mutations at 1,000 positions its 1,273 amino acid sequence were reported, posing a major challenge...
A major challenge to end the pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is develop a broadly protective vaccine that elicits long-term immunity. As key immunogen, viral surface spike (S) protein frequently mutated, and conserved epitopes are shielded glycans. Here, we revealed S glycosylation has site-differential effects on infectivity. We found generated lung epithelial cells glycoforms associated with increased Compared fully glycosylated protein,...
RNA-protein interaction plays an essential role in several biological processes, such as protein synthesis, gene expression, posttranscriptional regulation and viral infectivity. Identification of RNA-binding sites proteins provides valuable insights for biologists. However, experimental determination remains time-consuming labor-intensive. Thus, computational approaches prediction have become highly desirable. Extensive studies site led to the development methods. they could yield low...
Highly tensile-strained Ge thin films and quantum dots have the potential to be implemented for high mobility metal-oxide-semiconductor field-effect transistor channels long-wavelength optoelectronic devices. To obtain large tensile strain, has epitaxially grown on a material with larger lattice constant. We report growth of relaxed InxGa1−xAs epitaxial templates by metal-organic chemical vapor deposition. investigate methods achieve quality epitaxy III–V semiconductor surfaces, we studied...
The programmable one-pot oligosaccharide synthesis method was designed to enable the rapid of a large number oligosaccharides, using software Optimer search Building BLocks (BBLs) with defined relative reactivity values (RRVs) be used sequentially in reaction. However, there were only about 50 BBLs measured RRVs original library and could synthesize small oligosaccharides due RRV ordering requirement. Here, we increase include 154 validated more than 50,000 virtual predicted by machine...
Glycosylation is a highly complex modification influencing the functions and activities of proteins. Interpretation intact glycopeptide spectra crucial but challenging. In this paper, we present mass spectrometry-based automated identification platform (MAGIC) to identify peptide sequences glycan compositions directly from N-linked collision-induced-dissociation spectra. The Y1 (peptideY0 + GlcNAc) ion critical for correct analysis unknown glycoproteins, especially without prior knowledge...
Significance To contain the spread of SARS-CoV-2, mRNA vaccine viral spike protein has proven to be highly effective, although efficacy against emerging variants is decreasing. Here, we show that with deletion glycosites in RBD or especially S2 domain expose more conserved epitopes can used as a broadly protective wild type and concern. The translated from such was not properly folded, thus induced cell apoptosis strong T response. Our findings demonstrate importance glycosylation effect on...
A method of forming cubic phase (zinc blende) GaN (referred as c‐GaN) on a CMOS‐compatible on‐axis Si (100) substrate is reported. Conventional materials are hexagonal (wurtzite) h‐GaN) and possess very high polarization fields (∼MV/cm) along the common growth direction <0001>. Such large lead to undesired shifts (e.g., wavelength current) in performance photonic vertical transport electronic devices. The polarization‐free <001>, however, this thermodynamically unstable,...
Abstract Phase‐change memory devices have found applications in in‐memory computing where the physical attributes of these are exploited to compute places without need shuttle data between and processing units. However, nonidealities such as temporal variations electrical resistance a detrimental impact on achievable computational precision. To address this, promising approach is projecting phase configuration change material onto some stable element within device. Here, projection mechanism...
We have investigated the effect of interface formation and processing conditions Al2O3 on GaAs density distribution state charge in band gap. formed insulator/semiconductor using both atomic layer deposition (ALD) chemical vapor (CVD). In situ ALD, ex CVD aluminum oxide (Al2O3) were employed metal-organic CVD. Isopropanol (IPA) was chosen as oxygen source for deposition. No arsenic or gallium detected at ALD Al2O3/GaAs interface, while observed interface. The entire distributions interfacial...
Abstract Background Development of computational tools that can accurately predict presence and location B-cell epitopes on pathogenic proteins has a valuable application to the field vaccinology. Because highly variable yet enigmatic nature epitopes, their prediction presents great challenge immunologists. Methods We propose method, BEEPro ( B -cell e pitope by volutionary information pro pensity scales), which adapts linear averaging scheme 16 properties using support vector machine model...
In situ atomic-layer deposition (ALD) of Al2O3 on p-GaAs in metal-organic chemical vapor system is demonstrated this article. Isopropanol was chosen as the oxygen source for ALD, instead common H2O. The ALD mechanism discussed and it proposed that water does not form process. saturation growth rate about 0.8Å∕cycle. X-ray photoetectron spectroscopy depth profiles were performed no arsenic oxide observed at interface. capacitance-voltage measurements show a small accumulation capacitance...
The nonvolatile memory characteristics of metal-oxide-semiconductor structures containing Pt nanocrystals in SiO2 gate oxide were studied. 2–3nm diameter self-assembled from reduction an ultrathin PtOx layer embedded the by vacuum annealing at 425°C. A large hysteresis loop was found capacitance–voltage (C–V) relation indicating this significant effect. However, two different charge storage mechanisms for devices with tunnel thickness. counterclockwise C–V induced substrate injection made a...
Carbon nanofibers functionalized with aminobenzoyl groups (CNF-aminobenzoyl) were prepared via direct Friedel-Crafts acylation in polyphosphoric acid. The functionalization of CNFs was characterized using XPS, FTIR, TGA, and Raman analyses. Hexafluoroisopropylidene-containing polybenzimidazole (6FPBI) composite membranes containing pristine or CNF-aminobenzoyl solvent-assisted dispersion solvent-casting methods. In this work, the influence incorporation on several physicochemical properties...
Annealing native oxide covered GaAs samples in Arsine(AsH3) prior to atomic-layer-deposition of Al2O3 with trimethyaluminum (TMA) and isopropanol (IPA) results capacitance-voltage (C-V) characteristics the treated that resemble superior C-V p-type grown by an situ metal-organic chemical vapor deposition process. Both TMA IPA show self-cleaning effect on removing ex process, little evidence a was observed high resolution transmission electron microscopy at Al2O3/GaAs interface. The...
Since the outbreak of Severe Acute Respiratory Syndrome Virus-2 (SARS-CoV-2) in 2019, more than 15 million spike protein sequences have been identified, raising a new challenge for development broadly protective vaccine against various emerging variants. We found that virus, like most other human viruses, depends on host-made glycans to shield conserved epitopes from immune response and demonstrated deletion glycan shields exposed highly elicited responses. In this study, we identified 17 14...
α-Helical transmembrane (TM) proteins play an important role in many critical and diverse biological processes, specific associations between TM helices are determinants for membrane protein folding, dynamics function. In order to gain insights into the above phenomena, it is necessary investigate different types of helix-packing modes interactions. However, such information difficult obtain because experimental impediment a lack well-annotated source folds proteins. We have developed TMPad...
A quaternized polybenzimidazole (PBI) membrane was synthesized by grafting a dimethylimidazolium end-capped side chain onto PBI. The organic–inorganic hybrid of the PBI prepared via silane-induced crosslinking process with triethoxysilylpropyl chloride. chemical structure and morphology were characterized using NMR, FTIR, TGA, SEM, EDX, AFM, SAXS, XPS techniques. Compared pristine dimethylimidazolium-functionalized PBI, its exhibited lower swelling ratio, higher mechanical strength, better...
Abstract Background Since membrane protein structures are challenging to crystallize, computational approaches essential for elucidating the sequence-to-structure relationships. Structural modeling of proteins requires a multidimensional approach, and one critical geometric parameter is rotational angle transmembrane helices. Rotational angles helices characterized by their folded could be inferred hydrophobic moment; however, folding mechanism not yet fully understood. The helix related...
MAGIC-web is the first web server, to best of our knowledge, that performs both untargeted and targeted analyses mass spectrometry-based glycoproteomics data for site-specific N-linked glycoprotein identification. The two modules, MAGIC MAGIC+, are designed analysis, respectively. implemented with previously proposed novel Y1-ion pattern matching method, which adequately detects Y1- Y0-ion without prior information proteins glycans, then generates in silico MS(2) spectra serve as input a...
Identification of subcellular localization in proteins is crucial to elucidate cellular processes and molecular functions a cell. However, given tremendous amount sequence data generated the post-genomic era, determining protein based on biological experiments can be expensive time-consuming. Therefore, developing prediction systems analyze uncharacterised efficiently has played an important role high-throughput analyses. In eukaryotic cell, many essential take place nucleus. Nuclear shuttle...
In this paper, we demonstrate high electron mobility In0.53Ga0.47As quantum-well metal oxide semiconductor field effect transistor (MOSFET) structures. The Al2O3 (gate dielectric)/ In0.53Ga0.47As-In0.52Al0.48As (barrier)/In0.53Ga0.47As (channel) structures were fabricated, and the was obtained by Hall measurements. with in-situ chemical vapor deposition (CVD) displayed higher than identical fabricated in situ atomic layer Al2O3, which indicates that CVD process resulted a lower...