A5033917569- Pancreatic function and diabetes
- Advanced Proteomics Techniques and Applications
- Metabolism, Diabetes, and Cancer
- Alzheimer's disease research and treatments
- Neuroscience and Neuropharmacology Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Mass Spectrometry Techniques and Applications
- Diabetes and associated disorders
- Mitochondrial Function and Pathology
- Memory and Neural Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Glycosylation and Glycoproteins Research
- 14-3-3 protein interactions
- Galectins and Cancer Biology
- Autophagy in Disease and Therapy
- Computational Drug Discovery Methods
- Peptidase Inhibition and Analysis
- Advanced biosensing and bioanalysis techniques
- Regulation of Appetite and Obesity
- Phagocytosis and Immune Regulation
- Genetics, Aging, and Longevity in Model Organisms
- Endoplasmic Reticulum Stress and Disease
- Nuclear Receptors and Signaling
- Ubiquitin and proteasome pathways
- Biochemical Analysis and Sensing Techniques
Mayo Clinic in Arizona
2024
Mayo Clinic
2023-2024
WinnMed
2024
University of Southern Denmark
2017-2023
Catholic University of Korea
2023
Danish Diabetes Academy
2019-2021
Institute for Basic Science
2014-2015
Chungnam National University
2013-2014
Korea Basic Science Institute
2013-2014
Abstract Alzheimer’s disease (AD) is the most common form of dementia; however, mechanisms and biomarkers remain unclear. Here, we examined hippocampal CA4 dentate gyrus subfields, which are less studied in context AD pathology, post-mortem control tissue to identify possible biomarkers. We performed mass spectrometry-based proteomic analysis combined with label-free quantification for identification differentially expressed proteins. identified 4,328 proteins, 113 showed more than 2-fold...
Alzheimer's disease (AD) is characterized by progressive memory loss accompanied synaptic and neuronal degeneration. Although research has shown that substantial neurodegeneration occurs even during the early stages of AD, detailed mechanisms
Normal pancreatic islet β-cells (PBCs) abundantly secrete insulin in response to elevated blood glucose levels, order maintain an adequate control of energy balance and homeostasis. However, the molecular mechanisms underlying secretion are unclear. Improving our understanding glucose-stimulated (GSIS) under normal conditions is a prerequisite for developing better interventions against diabetes. Here, we aimed at identifying novel signaling pathways involved initial release from PBCs after...
The onset of obesity-linked type 2 diabetes (T2D) is marked by an eventual failure in pancreatic β-cell function and mass that no longer able to compensate for the inherent insulin resistance increased metabolic load intrinsic obesity. However, a commonly used model T2D,
Abstract The rapid evolution of mass spectrometry-based single-cell proteomics now enables the cataloging several thousand proteins from single cells. We investigated whether we could discover cellular heterogeneity beyond proteome, encompassing post-translational modifications (PTM), protein-protein interaction, and variants. By optimizing spectrometry data interpretation strategy to enable detection PTMs variants, have generated a high-definition dataset nuclear proteomic-states....
For the treatment of patients with prediabetes or diabetes, clinical evidence has emerged that β-cell function can be restored by glucose-lowering therapeutic strategies. However, little is known about molecular mechanisms underlying this functional adaptive behavior pancreatic β-cell. This study examines dynamic changes in protein expression and phosphorylation state associated (pro)insulin production secretory pathway mediated euglycemia to induce rest obese/diabetic db/db islet β-cells....
Altered nucleolar and ribosomal dynamics are key hallmarks of aging, but their regulation remains unclear. Building on the knowledge that conserved nuclear export receptor Exportin 1 (XPO-1/XPO1) modulates proteostasis life span, we systematically analyzed impact protein metabolism. Using transcriptomic subcellular proteomic analyses in nematodes, demonstrate XPO-1 nucleocytoplasmic distribution proteins involved ribosome function, including fibrillarin (FIB-1/FBL) RPL-11 (RPL11). Silencing...
Human chromosome 11 is the third gene-rich having 1304 protein-coding genes. According to GeneCards, this contains 240 genes related diseases, as it well known a disease-rich chromosome. Although there are many genes, proteomic identification ratio rather low. As model study, human hippocampal tissues from patients suffering Alzheimer's disease and epilepsy were prepared evaluate gene-centric statistics gene expression disorders of 11. A total 8828 protein coding brain extensively off-gel...
In this research, the potential anti-obesity efficacy of Lactobacillus rhamnosus BST-L.601 and its fermented product (named SPY) with mashed sweet potato paste were investigated using 3T3-L1 preadipocytes high-fat diet (HD)-induced obese mice. SPY (0-0.5 mg/mL) dose-dependently significantly reduced lipid accumulation TG content expression adipogenic markers (C/EBPα, PPAR-γ, aP2) fatty acid synthetic pathway proteins (ACC FAS) in adipocytes, demonstrating that suppresses adipocyte...
Microglial activation in the central nervous system is a key event neuroinflammation that accompanies neurodegenerative diseases such as Alzheimer's disease (AD). Among cytokines involved microglial activation, amyloid β (Aβ) peptide known to be molecule induction of diverse inflammatory products, which may lead chronic inflammation AD. However, proteomic studies microglia AD are limited due lack proper cell or animal model systems. In this study, we performed analysis Aβ-stimulated human...
Although tandem mass tag (TMT)-based isobaric labeling has become a powerful approach for multiplexed protein quantitation, automating the workflow this technique not been easy to achieve widespread adoption. This is because preparation of TMT-labeled peptide samples involves multiple steps ranging from extraction, denaturation, reduction, and alkylation tryptic digestion, desalting, labeling, cleanup, all which require high level proficiency. The variability resulting processing inherently...
The postsynaptic density (PSD) of excitatory synapses contains a highly organized protein network with thousands proteins and is key node in the regulation synaptic plasticity. To gain new mechanistic insight into experience-induced changes PSD, we examined global dynamics hippocampal PSD proteome phosphoproteome mice following four different types experience. Mice were trained using an inhibitory avoidance (IA) task fractions isolated from individual to investigate molecular mechanisms...
A comprehensive analysis of site-specific protein O-glycosylation is hindered by the absence a consensus motif, diversity O-glycan structures, and lack universal enzyme that cleaves attached O-glycans. Here, we report development robust O-glycoproteomic workflow for analyzing complex biological samples combining four different strategies: removal N-glycans, complementary digestion using O-glycoprotease (IMPa) with/without another protease, glycopeptide enrichment, mass spectrometry with...
Characterization of molecular mechanisms underlying pancreatic β-cell function in relation to glucose-stimulated insulin secretion is incomplete, especially with respect global response the nuclear environment. We focus on characterization proteins environment β-cells after brief, high glucose stimulation. compared purified nuclei derived from stimulated 17 mM for 0, 2, and 5 min using quantitative proteomics, a time frame that most likely does not result translation new protein cell. Among...
Pro-inflammatory cytokines promote cellular iron-import through enhanced divalent metal transporter-1 (DMT1) expression in pancreatic β-cells, consequently cell death. Inhibition of β-cell by DMT1 silencing protects against apoptosis animal models diabetes. However, how alterations signaling networks contribute to the protective action knock-down is unknown. Here, we performed phosphoproteomics using our sequential enrichment strategy mRNA, protein, and phosphopeptides, which enabled us...
Summary The post synaptic density (PSD) of excitatory synapses contains a highly organized protein network with thousands proteins and is key node in the regulation plasticity. To gain new mechanistic insight into experience-induced changes PSD, we examined global dynamics PSD proteome phosphoproteome mice following various treatments. Mice were trained using an inhibitory avoidance (IA) task hippocampal fractions isolated for quantitative proteomic phosphoproteomics analysis. We used...