Vibrio parahaemolyticus is the leading bacterial cause of food-borne illness due to consumption contaminated seafood. The aim present study was determine population its subtypes and establish a better understanding various types V. strains that are causing human in Canada. for 100 clinical isolates collected between 2000 2009 were determined by performing serotyping, ribotyping, pulsed-field gel electrophoresis, multilocus sequence typing. Within this panel strains, there high level...

Abstract Two methods were compared for their ability to accurately identify Vibrio species of interest: whole genome sequencing as the reference method and MALDI-TOF MS (matrix-assisted laser desorption/ionization-time flight mass spectrometry) proteome fingerprinting. The accuracy spectrometry–based identification was evaluated its isolates cholerae parahaemolyticus . Identification result each isolate obtained by spectrometry (WGS). system had excellent performance V. grown on a...

ABSTRACT Vibrio parahaemolyticus is a bacterial pathogen that can cause illness after the consumption or handling of contaminated seafood. The primary virulence factors associated with V. are thermostable direct hemolysin (TDH) and Tdh-related (TRH). However, clinical strains lacking tdh trh have recently been isolated, these isolates poorly understood. To help understand emergence - -negative isolates, genomic approach was used to comprehensively compare 4 16 environmental 34 positive for ,...

Geographic food fraud - misrepresenting the geographic origin of a item, is very difficult to detect, and therefore this type tends go undetected. This potentially negatively impacts health Canadians economic success our seafood industry. Surveillance studies have shown that up significant portion commercially sold items in Canada are mislabeled or otherwise misrepresented some way. The current study aimed determine if microbiome fresh shellfish could be used as an accurate marker harvest...

ABSTRACT Listeria innocua 743 produces an inhibitory activity demonstrating broad-spectrum inhibition of monocytogenes isolates. Gel-electrophoretic analysis culture supernatants indicated that two inhibitors with different molecular weights were produced by this strain. Insertion Tn 917 into a 2.9 Kb plasmid (pHC743) generated mutants either impaired ability or loss in to produce one the inhibitors. Sequence transposon insertion regions revealed presence continuous open reading frames,...

Chromosome number, C‐value and cell volume studies were carried out on three species of the genus Channa , viz., C. punctatus, striatus gachua . The chromosome karyotypic structure DNA content per along with are reported described. A series chromosomal rearrangements established in different karyotypes polyploidy. Both pericentric inversion Robertsonian fusion played a major role rearrangements. nuclear these is within 19‐29% present‐day placental mammals, thus lower than median amount for...

Vibrio species are indigenous to the marine and estuarine environments around world leading cause of water- seafood-borne illnesses due conditions favoring transmission growth species. Horizontal gene transfer, recombination, mutation enable spp. adapt rapidly environmental challenges from biotic abiotic parameters, including temperature, salinity, nutrient status coastal waters. This surveillance study provides evidence cholerae emerging in temperate estuaries Canada, thereby redefining...

Abstract Vibrio parahaemolyticus and V. vulnificus are bacterial foodborne pathogens that can cause illnesses in humans after ingestion or exposure to contaminated seafood coastal waters. Aprocedure combines microbiological, biochemical, molecular methods was designed optimized for thedetection, enumeration, isolation, characterization of these clinically significant spp. Initially, microbiological culturing is used resuscitate isolate presumptive from chilled samples. Biochemical tests then...

The emergence of antimicrobial resistance (AMR) in foodborne bacteria is a growing concern worldwide. AMR surveillance key element understanding the implications resulting from use antibiotics for therapeutic as well prophylactic needs. and spread human pathogens are indirect health hazards. This study reports trend pattern detected Vibrio species isolated molluscs harvested Canada between 2006 2012 against 19 commonly used antibiotics. Five common antibiotics, ampicillin, cephalothin,...

Vibrio parahaemolyticus is a leading cause of bacterial gastroenteritis following ingestion contaminated seafood. This report presents the draft genome sequences four clinical strains V. isolated in Canada. All lack traditional pathogenic markers and possess uniquely individual characteristics identified using other typing criteria.

Vibrio parahaemolyticus is a bacterial pathogen responsible for mild to severe gastroenteritis, wound infections, and septicemia resulting from the ingestion or handling of raw undercooked contaminated seafood. Here, we report draft whole-genome sequences annotations 14 Canadian V. clinical isolates that were serologically identified as K group II using polyvalent antisera but not specifically serogrouped monovalent antisera.

A 1,197-bp region of the broad-host-range plasmid pCU1 is adequate for its replication. Analysis replicating molecules containing this reveals three clustered origins vegetative replication and proceeds bidirectionally from each in a theta mode. In an Escherichia coli polymerase I deletion mutant, utilization one these was not detected. The potentiality origin may therefore be determinant replicon host range.

Whole genome sequencing (WGS) is rapidly replacing other molecular techniques for identifying and subtyping bacterial isolates. The resolution or discrimination offered by WGS significantly higher than that techniques, readily allows infrequent differences occur between 2 closely related strains to be found. In this investigation, was used identify the changes occurred in genomes of 13 foodborne pathogens after 100 serial subcultures. Pure cultures Shiga-toxin-producing Escherichia coli,...

ABSTRACT Human enteric pathogens in fresh uncooked seafood are of concern to human health. Here, we report the complete genomes bacteria not commonly found freshly harvested seafood, two Salmonella Typhimurium strains and one draft Escherichia coli genome, isolated from oysters grown Canada.

A subset of Vibrio spp. isolated from fresh Canadian mollusks (2014 to 2018) were selected for sequencing based on antimicrobial resistance profiles. The resulting de novo draft genomes include 38 alginolyticus, 32 V. diabolicus, 10 parahaemolyticus, 1 cholerae, ordalii, and sp. isolate.

Vibrio spp. isolated from fresh retail mollusk samples were selected for sequencing based on their antimicrobial resistance burden. The de novo genomes include those alginolyticus (n = 48), V. diabolicus 15), parahaemolyticus 3), cholerae 2), metoecus 1), vulnificus fluvialis and unidentified 4).

spp. were isolated from raw shrimps imported into Canada (2009 to 2019). A total of 92 isolates with various multidrug resistance profiles sequenced, including 59 V. parahaemolyticus, 12 diabolicus, 10 cholerae, 7 alginolyticus, 1 campbellii, harveyi, owensii, and vulnificus isolate.

The 2,053-bp broad-host-range incompatibility group N replicon of plasmid pCU1 has two components: a region 1,200 bp that is sufficient for its replication in Escherichia coli PolA+ and PolA- hosts regulatory called the I iteron contains 13 39-bp iterons. Within 1,200-bp region, there are three origins, which, oriB oriS, function third, oriV, which functions only hosts. also specifies protein RepA. We now show both oriS can delta polA strain but such strain, an absolute requirement without...