A5054475578- Antimicrobial Peptides and Activities
- Antimicrobial agents and applications
- Bacterial biofilms and quorum sensing
- Biochemical and Structural Characterization
- Vibrio bacteria research studies
- Bacterial Genetics and Biotechnology
- Orthopedic Infections and Treatments
- Polydiacetylene-based materials and applications
- Chemical Synthesis and Analysis
- Peptidase Inhibition and Analysis
- Probiotics and Fermented Foods
- Bone Tissue Engineering Materials
- Bacteriophages and microbial interactions
- Protein Hydrolysis and Bioactive Peptides
- Metal and Thin Film Mechanics
- Urban Design and Spatial Analysis
- Conservation Techniques and Studies
- Food, Nutrition, and Cultural Practices
- Escherichia coli research studies
- Microplastics and Plastic Pollution
- Nutrition, Genetics, and Disease
- Aquaculture disease management and microbiota
- Biofuel production and bioconversion
- Agricultural and Food Sciences
- Venomous Animal Envenomation and Studies
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2015-2024
Universidade do Porto
2012-2024
Institute of Mechanical Engineering and Industrial Mangement
2021-2022
Karolinska Institutet
2009-2012
Universidade Federal do Paraná
2004
Bacterial persistence in the environment and infected host is often aided by formation of exopolymer-enclosed communities known as biofilms. Heterogeneous gene expression takes place microcompartments formed within complex biofilm structure. This study describes cell differentiation an isogenic bacterial population based on example Salmonella enterica serovar Typhimurium. We analyzed major regulator CsgD at single-cell level with a chromosomal CsgD-green fluorescent protein (GFP)...
The RNA chaperone Hfq and its associated small RNAs (sRNAs) regulate a variety of phenotypes in bacteria. In this work, we show that is master regulator biofilm formation Salmonella enterica serovar Typhimurium. two Hfq-dependent sRNAs (ArcZ SdsR) are required for rdar morphotype expression S. typhimurium. controls through the major CsgD. While csgD mRNA steady-state levels altered sdsR mutant, ArcZ seems to work mainly at post-transcriptional level. Overexpression complemented an hfq mutant...
Abstract Urinary catheters are extensively used in hospitals, being responsible for about 75% of hospital-acquired infections. In this work, a de novo designed antimicrobial peptide (AMP) Chain201D was studied the context urinary catheter-associated showed excellent activity against relevant ATCC strains and clinical isolates bacteria yeast demonstrated high stability wide range temperatures, pH salt concentrations. Moreover, bactericidal immobilized on model surface Escherichia coli ( E . )...
Summary Bacterial species of the Enterobacteriaceae family produce cellulose and curli fimbriae as extracellular matrix components, their synthesis is positively regulated by transcriptional activator CsgD. In this group bacteria, biosynthesis commonly CsgD via GGDEF domain protein AdrA, a diguanylate cyclase that produces cyclic‐diguanylic acid (c‐di‐GMP), an allosteric synthase. probiotic Escherichia coli strain Nissle 1917 its recent clonal isolates, activates production at 28°C, but...
Cyclic di-GMP (c-di-GMP), a novel secondary signalling molecule present in most bacteria, controls transition between motility and sessility. In Salmonella enterica serovar Typhimurium (S. typhimurium) high c-di-GMP concentrations favour the expression of biofilm state through master regulator CsgD. this work, we investigate effect on virulence phenotypes S. typhimurium. After saturation cell with by overexpression di-guanylate cyclase, studied invasion induction pro-inflammatory cytokine...
In Salmonella enterica serovar Typhimurium, a biofilm mode of growth known as the rdar morphotype is regulated by several networks which sense multiple environmental signals. The transcriptional regulator CsgD major target for these regulatory pathways. this study, we show that two lytic transglycosylases family I, MltE and MltC, in combination increase expression morphotype. share highly similar transglycosylase SLT domain, work redundantly to regulate at posttranscriptional levels. effect...
Covalent conjugation of antimicrobial peptides (AMP) is considered an effective approach to overcome their potential degradation in vivo. As the high cost AMP-based therapies one major hurdles toward clinical application, it urgently needed explore yield reactions. Herein, highly efficient thiol–norbornene photoclick chemistry (TNPC) was explored for AMP grafting onto chitosan. Norbornenes were introduced chitosan (NorChit) aqueous/organic system, followed by UV-triggered N- and C-terminus...
Abstract As we approach the end of antibiotic era, newer therapeutic options, such as antimicrobial peptides (AMPs), are in urgent demand. AMP surface grafting onto biomaterials has been described a good strategy to overcome problems associated with their vivo stability. Helicobacter pylori is among bacteria that pose greatest threat human health, being MSI-78A one few bactericidal AMPs against this bacterium. Here, report grafted model surfaces (Self-Assembled Monolayers –SAMs),...
Wound infection treatment with antimicrobial peptides (AMPs) is still not a reality, due to the loss of activity in vivo. Unlike conventional strategy encapsulating AMPs on nanoparticles (NPs) leaving dependent release profile, this work explores AMP grafting poly(D,L-lactide-co-glycolide)-polyethylene glycol NPs (PLGA-PEG NPs), whereby exposition, targeting and immediate action are promoted. functionalized MSI-78(4-20), an equipotent more selective derivative MSI-78, grafted through...
Medical adhesives are essential to secure wound care dressings and medical devices the skin, bind edges, track vital signs, or even provide local drug delivery. Despite several options in market, most composed of acrylate, hydrocolloid, silicone, materials that associated with development Adhesive-Related Skin Injury (MARSI). Moreover, these injuries reduce skin integrity, causing pain, delaying healing, increasing risk infection, consequently extending treatment time, impairing quality life...
Surface modification of the Ti6Al4V alloy (ASTM grade 5), with fabrication vertically oriented TiO2 nanotubes, has been receiving increasing attention both as a way to provide advanced bioactive features and ability act reservoirs for localized, controlled drug release. In this work, nanotubes were grown on surface through electrochemical anodization. An ethylene glycol-based electrolyte containing 0.5 wt.% NH4F 2.5% (v/v) H2O was used. Post-anodizing heat treatments at 500 °C in air...
Antimicrobial peptides are widely recognized as an excellent alternative to conventional antibiotics. MSI-78, a highly effective and broad spectrum AMP, is one of the most promising AMPs for clinical application. In this study, we have designed shorter derivatives MSI-78 with aim improving selectivity while maintaining antimicrobial activity. Shorter 17-mer were created by truncating at N- and/or C-termini, spanning sequence. Despite truncations made, found peptide, MSI-78(4-20)...
Antibiotic resistance is increasing and new strategies are needed to fight infection. Advanced materials promising tools that can be combined with innovative alternatives conventional antibiotics allow more targeted efficient treatment. In this work, we explored the activity against Staphylococcus epidermidis (S. epidermidis) of α-helical antimicrobial peptide (AMP) MSI-78(4-20) (KFLKKAKKFGKAFVKIL) when covalently bound a chitosan coating. The AMP (17 mer) an improved version its parent...
Abstract Infections and thrombus formation are major concerns for the success of blood‐contacting medical devices. Antimicrobial coatings based on antimicrobial peptides (AMPs) described as promising strategies to fight biomaterial‐associated infections. However, their efficiency in presence plasma effect platelets adhesion/activation, essential blood contact applications, is not known. In this work, AMP cecropin–melittin (CM) covalently immobilized onto polyurethane (PU) films envisaging a...
It is key to fight bacterial adhesion prevent biofilm establishment on biomaterials. Surface immobilization of antimicrobial peptides (AMP) a promising strategy avoid colonization. This work aimed investigate whether the direct surface Dhvar5, an AMP with head-to-tail amphipathicity, would improve activity chitosan ultrathin coatings. The peptide was grafted by copper-catalyzed azide-alkyne cycloaddition (CuAAC) chemistry either its C- or N- terminus assess influence orientation properties...
Bacterial biofilms are a major health concern, mainly due to their contribution increased bacterial resistance well-known antibiotics. The conventional treatment of represents challenge, and frequently, eradication is not achieved with long-lasting administration In this context, the present work proposes an innovative therapeutic approach that focused on encapsulation N-acetyl-l-cysteine (NAC) into lipid nanoparticles (LNPs) functionalized d-amino acids target disrupt biofilms. optimized...
Abstract Background The World Health Organization has identified Helicobacter pylori , a Gram‐negative bacterium responsible for several gastric disorders, as one of the pathogenic bacteria that requires newer non‐antibiotic approaches its management. We previously demonstrated nanostructured lipid carriers (NLC) loaded with docosahexaenoic acid (DHA‐NLC) have excellent in vitro performance against H. . Materials and Methods NLC were tested different strains representative from human gut...
Infection is one of the main causes orthopedic implants failure, with antibiotic-resistant bacteria playing a crucial role in this outcome. In work, antimicrobial nanogels were developed to be applied situ as implant coating prevent orthopedic-device-related infections. To that regard, broad-spectrum peptide, Dhvar5, was grafted onto chitosan via thiol–norbornene "photoclick" chemistry. Dhvar5-chitosan (Dhvar5-NG) then produced using microfluidic system. Dhvar5-NG (1010 (NG)/mL) Dhvar5...
Infection is one of the major issues associated with failure orthopedic devices, mainly due to implant bacterial colonization, biofilm formation, and antibiotic resistance. Antimicrobial peptides (AMP) are a promising alternative conventional antibiotics given their broad-spectrum activity, low propensity induce resistance, ability modulate host immune responses. Dhvar5 (LLLFLLKKRKKRKY) MSI78 (GIGKFLKKAKKFGKAFVKILKK) two AMP activity against bacteria, including methicillin-resistant...
Efficient antibiotics are being exhausted, which compromises the treatment of infections, including complicated skin and structure infections (cSSTI) often associated with multidrug resistant (MDR) bacteria, methicillin-resistant S. aureus (MRSA) most prevalent. Antimicrobial peptides (AMP) increasingly regarded as new hope for post-antibiotic era. Thus, future management cSSTI may include use that, on one hand, behave AMP and, other, able to promote fast correct rebuilding. As such, we...
As life expectancy increases, diseases causing chronic wound infections become more prevalent. Diabetes, peripheral vascular diseases, and bedridden patients are often associated with non-healing wounds that infected, resulting in high morbidity mortality.