A5066191313- Antimicrobial agents and applications
- Antimicrobial Peptides and Activities
- Silk-based biomaterials and applications
- Polydiacetylene-based materials and applications
- 3D Printing in Biomedical Research
- Wound Healing and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Luminescence and Fluorescent Materials
- Marine Sponges and Natural Products
- Microbial Metabolites in Food Biotechnology
- Nanoplatforms for cancer theranostics
- Supramolecular Self-Assembly in Materials
- Angiogenesis and VEGF in Cancer
- Inhalation and Respiratory Drug Delivery
- Biochemical and Structural Characterization
- Microfluidic and Bio-sensing Technologies
- Cell Image Analysis Techniques
Universidade do Porto
2021-2024
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2019-2024
Rede de Química e Tecnologia
2021-2024
Surface bioconjugation of antimicrobial peptides (AMP) onto nanoparticles (AMP-NP) is a complex, multistep, and time-consuming task. Herein, microfluidic system for the one-pot production AMP-NP was developed. Norbornene-modified chitosan used NP (NorChit-NP), thiolated-AMP grafted on their surface via thiol–norbornene "photoclick" chemistry over exposure two parallel UV LEDs. The MSI-78A AMP selected due to its high activity against priority (level 2) antibiotic-resistant gastric pathogen:...
Covalent conjugation of antimicrobial peptides (AMP) is considered an effective approach to overcome their potential degradation in vivo. As the high cost AMP-based therapies one major hurdles toward clinical application, it urgently needed explore yield reactions. Herein, highly efficient thiol–norbornene photoclick chemistry (TNPC) was explored for AMP grafting onto chitosan. Norbornenes were introduced chitosan (NorChit) aqueous/organic system, followed by UV-triggered N- and C-terminus...
Impaired angiogenesis in skin chronic wounds prolongs inflammation and compromises wound healing. Several strategies have been attempted to improve vascularization, such as application of growth factors, like the vascular endothelial factor (VEGF). However, VEGF is expensive, has a short half-life vivo associated tumorigenesis when used high concentrations. QK peptide shorter (15 amino acids), synthetic, VEGF-mimetic with improved stability lower production cost. residence time may be...
A novel blue-emitting fluorescent alginate derivative has been successfully synthesized in a simple two-reaction step protocol. The developed material showed to be biocompatible and traceable upon long periods of incubation physiologic conditions.
It is key to fight bacterial adhesion prevent biofilm establishment on biomaterials. Surface immobilization of antimicrobial peptides (AMP) a promising strategy avoid colonization. This work aimed investigate whether the direct surface Dhvar5, an AMP with head-to-tail amphipathicity, would improve activity chitosan ultrathin coatings. The peptide was grafted by copper-catalyzed azide-alkyne cycloaddition (CuAAC) chemistry either its C- or N- terminus assess influence orientation properties...