A5059989403- Antimicrobial Peptides and Activities
- Antimicrobial agents and applications
- Helicobacter pylori-related gastroenterology studies
- Galectins and Cancer Biology
- Electrospun Nanofibers in Biomedical Applications
- Silk-based biomaterials and applications
- Microbial Metabolites in Food Biotechnology
- Advanced Drug Delivery Systems
- Renal and related cancers
- Bone Tissue Engineering Materials
- Toxin Mechanisms and Immunotoxins
- Biochemical and Structural Characterization
- Orthopedic Infections and Treatments
- Microencapsulation and Drying Processes
- Tissue Engineering and Regenerative Medicine
- Clostridium difficile and Clostridium perfringens research
- Probiotics and Fermented Foods
- Graphene and Nanomaterials Applications
- Polydiacetylene-based materials and applications
- 3D Printing in Biomedical Research
- Testicular diseases and treatments
- Marine Sponges and Natural Products
- Drug Solubulity and Delivery Systems
- Angiogenesis and VEGF in Cancer
- Inhalation and Respiratory Drug Delivery
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2021-2025
Universidade do Porto
2021-2024
University of Minho
2018-2019
Materials Research Group (United States)
2018
The rise in antibiotic resistance rate challenges the effective treatment of various bacterial infections. One them is gastric infection caused by Helicobacter pylori, a Gram-negative bacterium responsible for several disorders, as peptic ulcer, and close to 90 % all cancer cases. Available treatments, combining at least 3 antibiotics, fail up 40 patients, prompting need newer therapies. Bioengineered strategies, namely using biomaterials form micro/nanoparticles (MP/NP) have been explored...
Surface bioconjugation of antimicrobial peptides (AMP) onto nanoparticles (AMP-NP) is a complex, multistep, and time-consuming task. Herein, microfluidic system for the one-pot production AMP-NP was developed. Norbornene-modified chitosan used NP (NorChit-NP), thiolated-AMP grafted on their surface via thiol–norbornene "photoclick" chemistry over exposure two parallel UV LEDs. The MSI-78A AMP selected due to its high activity against priority (level 2) antibiotic-resistant gastric pathogen:...
Non-orthogonal scaffolds positively influenced the osteogenic performance of a Saos-2 cell line, presenting larger amount calcium phosphate deposition.
Impaired angiogenesis in skin chronic wounds prolongs inflammation and compromises wound healing. Several strategies have been attempted to improve vascularization, such as application of growth factors, like the vascular endothelial factor (VEGF). However, VEGF is expensive, has a short half-life vivo associated tumorigenesis when used high concentrations. QK peptide shorter (15 amino acids), synthetic, VEGF-mimetic with improved stability lower production cost. residence time may be...
Infection is one of the main causes orthopedic implants failure, with antibiotic-resistant bacteria playing a crucial role in this outcome. In work, antimicrobial nanogels were developed to be applied situ as implant coating prevent orthopedic-device-related infections. To that regard, broad-spectrum peptide, Dhvar5, was grafted onto chitosan via thiol–norbornene "photoclick" chemistry. Dhvar5-chitosan (Dhvar5-NG) then produced using microfluidic system. Dhvar5-NG (1010 (NG)/mL) Dhvar5...
Infection is still one of the main causes orthopedic implants failure. With alarming increase antibiotic-resistant bacteria, antimicrobial peptides (AMP) emerge as a future therapeutic alternative. In this work, Dhvar5, an AMP with broad-spectrum activity, was grafted to chitosan (Dhvar5-Chitosan), and nanogels (Dhvar5-NG) were produced using microfluidic system. We hypothesize that can be injected during implantation used create implant nanocoating will prevent bacterial colonization...
The action of bioactive peptides, such as antimicrobial peptides (AMP), in the human body is often compromised by limited residence time and stability target site. Bioconjugation to biomaterial surfaces one strategies that may overcome these limitations. Herein, norbornene-chitosan (NorChit) microspheres were engineered react with thiolated thiol-ene “photoclick” chemistry. NorChit produced spray drying crosslinked dithiothreitol (DTT) prevent their solubilization. Microspheres a diameter 5...