A5081236895- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Additive Manufacturing and 3D Printing Technologies
- Tissue Engineering and Regenerative Medicine
- Innovative Microfluidic and Catalytic Techniques Innovation
- Renal and related cancers
- Bone Tissue Engineering Materials
- Graphene and Nanomaterials Applications
- Spine and Intervertebral Disc Pathology
- Musculoskeletal pain and rehabilitation
- Renal cell carcinoma treatment
- Medical Imaging and Analysis
- Pluripotent Stem Cells Research
- Cervical and Thoracic Myelopathy
- Orthopaedic implants and arthroplasty
- Anesthesia and Pain Management
- Osteoarthritis Treatment and Mechanisms
- Cell Image Analysis Techniques
- Birth, Development, and Health
- Hydrogels: synthesis, properties, applications
- Anatomy and Medical Technology
- Pregnancy and preeclampsia studies
University of Aveiro
2022-2025
University of Minho
2017-2024
Materials Research Group (United States)
2018
Kidney regeneration is hindered by the limited pool of intrinsic reparative cells. Advanced therapies targeting renal have potential to alleviate clinical and financial burdens associated with kidney disease. Delivery systems for cells, extracellular vesicles, or growth factors aimed at enhancing can benefit from vehicles enabling targeted delivery controlled release. Hydrogels, optimized carry biological cargo while promoting regeneration, emerged as promising candidates this purpose. This...
Decellularized extracellular matrices (ECMs) are able to provide the necessary and specific cues for remodeling maturation of tissue-specific cells. Nevertheless, their use typical biofabrication applications requires chemical modification or mixing with other polymers, mainly due limited viscoelastic properties. In this study, we hypothesize that a bioink exclusively based on decellularized kidney ECM (dKECM) could be used bioprint renal progenitor To address these aims, porcine kidneys...
The aim of this work was to determine the influence biomaterial environment on human mesenchymal stem cell (hMSC) fate when cultured in supports with varying topography. Poly(vinylidene fluoride) (PVDF) culture were prepared structures ranging between 2D and 3D, based PVDF films which microspheres deposited surface density. Maintenance multipotentiality expansion medium studied by flow cytometry monitoring expression characteristic hMSCs markers, revealed that cells losing their markers...
Non-orthogonal scaffolds positively influenced the osteogenic performance of a Saos-2 cell line, presenting larger amount calcium phosphate deposition.
Abstract Granular inks comprising jammed hydrogel unit building blocks are emerging as multiprogramable precursors for 3D/4D printing nonbulk constructs. In addition to their injectability, they also exhibit high porosity when compared bulk hydrogels, allowing more efficient nutrient transport and cell migration through the scaffold structure. Herein, key steps in production of these inks, from fabrication microgels, jamming process, how affects final material properties, such porosity,...
Decellularized matrices are attractive substrates, being able to retain growth factors and proteins present in the native tissue.
Abstract Three-dimensional printing has risen in recent years as a promising approach that fast-tracked the biofabrication of tissue engineering constructs most resemble utopian tissue/organ replacements for precision medicine. Additionally, by using human-sourced biomaterials engineered towards optimal rheological proprieties extrudable inks, best possible scaffolds can be created. These encompass native structure and function with low risk rejection, enhancing overall clinical outcomes;...
Abstract Protein‐based hydrogels have great potential to be used as bioinks for biofabrication‐driven tissue regeneration strategies due their innate bioactivity. Nevertheless, use in conventional 3D bioprinting is impaired intrinsic low viscosity. Using embedding bioprinting, a liquid bioink printed within support that physically holds the patterned filament. Inspired by recognized microencapsulation technique complex coacervation, crystal self‐healing (CLADDING) introduced based on highly...
Abstract Aim Herein we propose creating a bilayer tubular kidney in‐vitro model. It is hypothesized that membranes composed of decellularized porcine extracellular matrix are valid substitutes the basement membrane by mimicking physiological relevance in vivo environment and disease phenotypes. Methods Extracellular was obtained from kidneys. After processing lyophilization milling, it dissolved an organic solvent blended with poly(caprolactone). Porous were electrospinning seeded human...
Abstract Intervertebral disc (IVD) herniation is a prevalent spinal disorder, often necessitating surgical intervention such as microdiscectomy for symptomatic relief and nerve decompression. IVDs comprise gel‐like nucleus pulposus (NP) encased by an annulus fibrosus (AF), their avascular nature renders them immune‐privileged. Microdiscectomy exposes the residual NP to immune system, precipitating cell infiltration attack that exacerbates IVD degeneration. While many efforts in tissue...
Abstract This study proposes a tunable ink engineering methodology to allow 3D printing processability of highly bioactive but otherwise low‐viscous and unprintable blood‐derived materials. The hypothesis relies on improving the viscoelasticity shear thinning behavior platelet lysates (PL) albumins (BSA) solutions by covalent coupling, enabling simultaneous extrusion photocrosslinking upon filament deposition. available amine groups proteins (PL BSA) are exploited for coupling with carboxyl...
Difficulties during the wound healing process may result in scarring, chronic wounds and sepsis. A common tissue engineering strategy to solve these problems rely on development of 3D hydrogel scaffolds that mimic structure, stiffness, biological proprieties target tissue. One most effective biofabrication techniques precisely control spatial deposition, architecture porosity hydrogels is printing technology. However, final architectures printed structures can be compromised if properties...
The intricate porous network of cryogels improves injectability and shape-recovery properties, offering a valuable tool for minimal invasive biomedical applications. unique diffusivity properties as compared with conventional hydrogels makes them highly attractive different These pores are able to improve cell migration, but the injection cell-loaded complex structures remains challenging. Here, we demonstrate freeform 3D printing injectable macroporous cell-adsorptive shape-recoverability...
Abstract Protein-based hydrogels have great potential to be used as bioinks for biofabrication-driven tissue regeneration strategies due their innate bioactivity. Nevertheless, use in conventional 3D bioprinting is impaired intrinsic low viscosity. Using embedding bioprinting, a liquid bioink printed whithin support that physically holds the patterned filament. Inspired by recognized microencapsulation technique complex coacervation, we introduce crystal self-healing (CLADDING) based on...
Introduction Ink engineering can advance 3D-printability for better therapeutics, with optimized proprieties. Herein, we describe a methodology yielding 3D-printable nanocomposite inks (NC) using low-viscous matrices, via the interaction between organic and inorganic phases by chemical coupling. Method Natural photocurable matrices were synthesized: protein – bovine serum albumin methacrylate (BSAMA), polysaccharide hyaluronic acid (HAMA). Bioglass nanoparticles (BGNP) synthesized...