A5080557733- Bone Tissue Engineering Materials
- Polymer Surface Interaction Studies
- RNA Interference and Gene Delivery
- Nerve injury and regeneration
- Advanced Drug Delivery Systems
- Osteoarthritis Treatment and Mechanisms
- Cell Adhesion Molecules Research
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Silk-based biomaterials and applications
- Graphene and Nanomaterials Applications
- Periodontal Regeneration and Treatments
- 3D Printing in Biomedical Research
- Connective tissue disorders research
- Cellular Mechanics and Interactions
- biodegradable polymer synthesis and properties
- Supramolecular Self-Assembly in Materials
- Neurogenetic and Muscular Disorders Research
- Pluripotent Stem Cells Research
- Nanocomposite Films for Food Packaging
- Neurogenesis and neuroplasticity mechanisms
- Immune cells in cancer
- Conducting polymers and applications
- Connective Tissue Growth Factor Research
- Muscle Physiology and Disorders
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2015-2019
Universidade do Porto
2009-2019
Institute of Mechanical Engineering and Industrial Mangement
2005-2013
Polytechnic Institute of Viseu
2008
In the present work, surface of chitosan membranes was modified using a phosphorylation method carried out at room temperature. Phosphorylation may be particular interest in materials for orthopaedic applications, due to cation-exchange properties phosphate functionalities. Phosphate groups chelate calcium ions, thus inducing deposition an apatite-like layer known improve osteoconduction polymer-based implants. Additionally, negatively charged functionalities, together with positively amine...
The use of biomaterials to replace lost bone has been a common practice for decades. More recently, the demands repair and regeneration have pushed research into cultured cells growth factors in association with these materials. Here we report novel approach engineer new using transient cartilage scaffold induce endochondral ossification. Chondrocyte/chitosan scaffolds (both scaffold-experimental-and permanent scaffold-control) were prepared implanted subcutaneously nude mice. Bone formation...
Synthetic designer matrix enhances muscle stem cell function and engraftment in injured dystrophic aged skeletal muscles.
Chitosan (Ch) is being actively investigated as a non-protein template for the growth of an increasing number anchorage-dependent cells, including chondrocytes and bone cells. In present work, Ch films with degrees N-acetylation (DAs) in range 4 to 49% were evaluated respect attachment, spreading short-term proliferation osteoblasts, using human osteoblastic MG-63 The characterized terms surface morphology charge by atomic force microscopy streaming potential measurements, respectively. Cell...
Abstract The effect of the degree acetylation (DA) 3D chitosan (Ch) scaffolds on inflammatory reaction was investigated. Chitosan porous with DAs 4 and 15% were implanted using a subcutaneous air‐pouch model inflammation. initial acute response evaluated 24 48 h after implantation. To characterize response, recruitment adhesion cells to implant site studied. fibrous capsule formation infiltration within for longer implantation times (2 weeks). DA attracted highest number leukocytes site....
Abstract In this investigation, the effect of degree acetylation (DA) chitosan on behavior human osteoblastic MG‐63 cells cultured in three‐dimensional matrices was assessed. Chitosan sponges with DAs range 4 to 49% were prepared and characterized terms microstructure, porosity, pore size. Collagen used as 3D control. Cell proliferation determined using MTT assay while retention phenotype monitored by assaying alkaline phosphatase activity. morphology, cytoskeletal organization, viability...
Abstract Cell adhesion, migration, and proliferation of a few anchorage‐dependent cells cultured on chitosan (Ch) matrices are influenced by the degree N ‐acetylation (DA) Ch. In present work, we examined influence DA attachment, spreading, proliferation, osteogenic differentiation rat bone marrow stromal (rBMSCs). Ch membranes were characterized in terms surface morphology, roughness, wettability, adsorption an adhesive protein, fibronectin (Fn). Chs with DAs range 4 to 49% used. Among...
Degradable synthetic hydrogels with site-selective immobilized laminin constitute attractive platforms for hNSC culture in 3D or cell transplantation.
Abstract Bioabsorbable materials are of great interest for bone regeneration applications, since they able to degrade gradually as new tissue is formed. In this work, a fully biodegradable composite material containing polylactic acid (PLA) and calcium phosphate (CaP) soluble glass particles has been characterized in terms surface properties cell response. Cell cultures were performed direct contact with the also their extracts, evaluated using MTT assay, alkaline phosphatase activity,...
Chitosan scaffolds have been shown to possess biological and mechanical properties suitable for tissue engineering clinical applications. In the present work, chitosan sponges were evaluated regarding their ability support cartilage cell proliferation maturation, which are first steps in endochondral bone formation. seeded with chondrocytes isolated from chicken embryo sterna. Chondrocyte/chitosan constructs cultured 20 days, treated retinoic acid (RA) induce chondrocyte maturation matrix...
In an attempt to improve the efficacy of neural stem/progenitor cell (NSPC) based therapies, fibrin hydrogels are being explored provide a favourable microenvironment for survival and differentiation following transplantation. present work, ability support survival, proliferation, neuronal NSPCs derived from embryonic stem (ES) cells under monolayer culture was explored. Single mouse ES-NSPCs were cultured within (fibrinogen concentration: 6 mg/ml) conditions up 14 days. The retained high...
Abstract Grafting negatively charged groups such as phosphates is a well‐known strategy for inducing the deposition of apatite‐like layers under simulated physiological conditions. In this investigation, chitosan was phosphorylated in an attempt to enhance its osteoconduction. Chitosan membranes were at 30°C with H 3 PO 4 /Et /P 2 O 5 /butanol reaction system periods up 48 h. This method alternative phosphoric acid/urea/dimethylformamide phosphorylation method, which involves use much higher...
After spinal cord injury (SCI), nerve regeneration is severely hampered due to the establishment of a highly inhibitory microenvironment at site, through contribution multiple factors. The potential antisense oligonucleotides (AONs) modify gene expression different levels, allowing regulation cell survival and function, together with availability chemically modified nucleic acids favorable biopharmaceutical properties, make AONs an attractive tool for novel SCI therapy developments. In this...