A5011950033- Polyamine Metabolism and Applications
- Head and Neck Cancer Studies
- Radiomics and Machine Learning in Medical Imaging
- RNA and protein synthesis mechanisms
- Cancer-related gene regulation
- RNA modifications and cancer
- Amino Acid Enzymes and Metabolism
- RNA Research and Splicing
- Fungal and yeast genetics research
- Natural product bioactivities and synthesis
- Bacteriophages and microbial interactions
- Plant biochemistry and biosynthesis
- Genomics and Chromatin Dynamics
- Ubiquitin and proteasome pathways
- Vibrio bacteria research studies
- Probiotics and Fermented Foods
- Enzyme Production and Characterization
- Natural Compounds in Disease Treatment
- Biofuel production and bioconversion
- interferon and immune responses
- Oral microbiology and periodontitis research
- Transgenic Plants and Applications
- Trypanosoma species research and implications
- Helicobacter pylori-related gastroenterology studies
- Chemical and Physical Properties in Aqueous Solutions
Universidade Estadual Paulista (Unesp)
2016-2025
Instituto de Ciências Farmacêuticas
2011-2023
Centro Universitário de Araraquara
1993-2020
Universidade de São Paulo
1994-2018
Dana-Farber Cancer Institute
1998-2002
Harvard University
1998-2002
Universidade Federal de São Paulo
2000-2001
Universidade Estadual de Campinas (UNICAMP)
2000-2001
Instituto Butantan
2000
Eukaryotic mRNA processing and export is mediated by various heterogeneous nuclear ribonucleoproteins (hnRNPs). Many of these hnRNPs are methylated on arginine residues. In the yeast, Saccharomyces cerevisiae, predominant enzyme responsible for methylation Hmt1p. Hmt1p methylates both Npl3p Hrp1p, which shuttling involved in export. Here, we employ an vivo assay to show that important hnRNPs. Both Hrp1p fail exit nucleus cells lacking Hmt1p, overexpression enhances The a novel hnRNP-like...
Recurrent respiratory infections (RRIs) are a common clinical condition in children, fact about 25% of children under 1 year and 6% during the first 6 years life have RRIs. In most cases, occur with mild manifestations frequency episodes tends to decrease over time complete resolution by 12 age. However, RRIs significantly reduce child family quality lead significant medical social costs.Despite importance this condition, there is currently no agreed definition term literature, especially...
Open reading frame expressed sequences tags (ORESTES) differ from conventional ESTs by providing sequence data the central protein coding portion of transcripts. We generated a total 696,745 ORESTES 24 human tissues and used subset that correspond to set 15,095 full-length mRNAs as means assessing efficiency strategy its potential contribution definition transcriptome. estimate sampled over 80% all highly moderately expressed, between 40% 50% rarely genes. In our most thoroughly sequenced...
Recent work has demonstrated that some actively transcribed genes closely associate with nuclear pore complexes (NPC) at the periphery. The Saccharomyces cerevisiae Mlp1 and Mlp2 proteins are components of inner basket mediate interactions these active genes. To investigate physical link between NPC loci, we identified interact carboxyl-terminal globular domain by tandem affinity purification coupled mass spectrometry. This analysis led to identification several...
The nuclear poly(A)-binding protein 1 (PABPN1) is a ubiquitously expressed that plays critical role in polyadenylation. Short expansions of the polyalanine tract N-terminus PABPN1 lead to oculopharyngeal muscular dystrophy (OPMD), which an adult onset disease characterized by eyelid drooping, difficulty swallowing and weakness proximal limb muscles. Although significant data from vitro biochemical assays define function control poly(A) tail length, little known about mammalian cells. To...
Head and neck squamous cell carcinoma (HNSCC) is a heterogeneous disease affecting the epithelium of oral cavity, pharynx larynx. Conditions most patients are diagnosed at late stages disease, no sensitive specific predictors aggressive behavior have been identified yet. Therefore, early detection prognostic biomarkers highly desirable for more rational management disease. Hypermethylation CpG islands one important epigenetic mechanisms that leads to gene silencing in tumors has extensively...
Background: Cardiovascular disease is the leading cause of worldwide morbidity and mortality. Several studies have demonstrated that specific probiotics affect host’s metabolism may influence cardiovascular risk. Objectives: The aim this study was to investigate an isoflavone-supplemented soy product fermented with Enterococcus faecium CRL 183 Lactobacillus helveticus 416 on risk markers in moderately hypercholesterolemic subjects. Design: Randomized placebo-controlled double-blind trial...
Abstract The highly conserved eukaryotic translation initiation factor eIF5A has been proposed to have various roles in the cell, from mRNA decay nuclear protein export. To further our understanding of this essential protein, three temperature-sensitive alleles yeast TIF51A gene characterized. Two mutant proteins contain mutations a proline residue at junction between two domains and third, strongest allele encodes with single mutation each domain, both which are required for growth defect....
A complexity of pathway expression in yeast compared to prokaryotes is the need for separate promoters and terminators each gene expressed. Single transcript separated protein production possible via use 2A viral peptides, but detailed characterization assess their suitability applications needed. The present work aimed characterize multiple peptide sequences determine metabolic engineering Saccharomyces cerevisiae. We screened 22 peptides placed between fluorescent sequences. Cleaving...
The eukaryotic translation initiation factor 5A (eIF5A) is the only protein that contains hypusine [ N ε ‐(4‐amino‐2‐hydroxybutyl)lysine], which required for its activity. Hypusine formed by post‐translational modification of one specific lysine (Lys50 human eIF5A) deoxyhypusine synthase and hydroxylase. To investigate features eIF5A activity, we generated 49 mutations in eIF5A‐1, with a single amino acid substitution at highly conserved residues or N‐terminal C‐terminal truncations, tested...
Background Some probiotic strains have the potential to assist in relieving symptoms of inflammatory bowel disease. The impact daily ingestion a soy-based product fermented by Enterococcus faecium CRL 183 and Lactobacillus helveticus 416 with addition Bifidobacterium longum ATCC 15707 on chemically induced colitis has been investigated thereof within period 30 days. Methods Colitis was dextran sulfate sodium. animals were randomly assigned into five groups: Group C: negative control; CL:...
Abstract Among the biologically active triterpenes, friedelin has most-rearranged structure produced by oxidosqualene cyclases and is only one containing a cetonic group. In this study, we cloned functionally characterized synthase cycloartenol from Maytenus ilicifolia (Celastraceae). The complete coding sequences of these 2 genes were leaf mRNA, their functions heterologous expression in yeast. sequence very similar to other known OSCs type (approximately 80% identity), although M. amino...
Hrp1p is a heterogeneous ribonucleoprotein (hnRNP) from the yeast Saccharomyces cerevisiae that involved in cleavage and polyadenylation of 3'-end mRNAs mRNA export. In addition, Hrplp one several RNA-binding proteins are posttranslationally modified by methylation at arginine residues. By using functional recombinant Hrp1p, we have identified RNA sequences with specific high affinity binding sites. These sites correspond to efficiency element for formation, UAUAUA. To examine effect on...
Transcribed sequences in the human genome can be identified with confidence only by alignment derived from cDNAs synthesized naturally occurring mRNAs. We constructed a set of 250,000 that represent partial expressed gene and are biased toward central coding regions resulting transcripts. They termed ORF sequence tags (ORESTES). The ORESTES were assembled into 81,429 contigs. Of these, 1,181 (1.45%) found to match chromosome 22 at least one contig for 162 (65.6%) 247 known genes, 67 (44.6%)...
Abstract A detailed genome mapping analysis of 213,636 expressed sequence tags (EST) derived from nontumor and tumor tissues the oral cavity, larynx, pharynx, thyroid was done. Transcripts matching known human genes were identified; potential new splice variants flagged subjected to manual curation, pointing 788 putatively alternative splicing isoforms, majority (75%) being insertion events. subset 34 isoforms (5% events) selected 23 (68%) confirmed by reverse transcription–PCR DNA...
eIF5A is a highly conserved putative eukaryotic translation initiation factor that has been implicated in initiation, nucleocytoplasmic transport, mRNA decay, and cell proliferation, but with no precise function assigned so far. We have previously shown high-copy PKC1 suppresses the phenotype of tif51A-1, temperature-sensitive mutant S. cerevisiae. Here, an attempt to further understand how Pkc1 functionally interacts eIF-5A, it was determined suppression tif51A-1 independent integrity MAP...