A5026681511- Corneal Surgery and Treatments
- Corneal surgery and disorders
- Glaucoma and retinal disorders
- Ocular Surface and Contact Lens
- Circadian rhythm and melatonin
- Retinal Development and Disorders
- Photoreceptor and optogenetics research
- Retinal Diseases and Treatments
- Advanced Glycation End Products research
- Retinal Imaging and Analysis
- Pesticide Exposure and Toxicity
- S100 Proteins and Annexins
- Laser Applications in Dentistry and Medicine
- Light effects on plants
- Intraocular Surgery and Lenses
- Environmental Toxicology and Ecotoxicology
- Blood properties and coagulation
- Protease and Inhibitor Mechanisms
- Proteoglycans and glycosaminoglycans research
- Wound Healing and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Retinopathy of Prematurity Studies
- Retinoids in leukemia and cellular processes
- Sulfur Compounds in Biology
- Cancer, Hypoxia, and Metabolism
Government Medical College and Hospital
2025
Harry S. Truman Memorial Veterans' Hospital
2016-2024
University of Missouri
2016-2024
Medical College of Wisconsin
2021-2024
Missouri College
2015-2024
University of Missouri Health System
2018-2019
Columbia University
2019
Singapore Eye Research Institute
2010-2017
Duke-NUS Medical School
2012-2016
National University of Singapore
2012-2015
Purpose.: The present study was designed to understand the role of inflammatory cytokines secreted by corneal epithelial cells in keratoconus (KC) and response treatment with cyclosporine A (CyA). Methods.: involved 129 Indian KC patients clinically graded according Amsler-Krumeich classification 20 healthy, nonectatic subjects as controls. Tear levels matrix metalloproteinase-9 (MMP9), interleukin-6 (IL6), tumor necrosis factor-α (TNFα) were measured using ELISA kits. Gene expression qPCR...
In mammals, the melanopsin gene (Opn4) encodes a sensory photopigment that underpins newly discovered inner retinal photoreceptors. Since its first discovery in Xenopus laevis and subsequent description humans mice, genes have been described all vertebrate classes. Until now, of these sequences considered representatives single orthologous (albeit with duplications teleost fish). Here, we describe functional characterisation new fish, bird, amphibian genomes, demonstrating that, fact,...
Abstract The avian retina and pineal gland contain autonomous circadian oscillators photo‐entrainment pathways, but the photopigment(s) that mediate entrainment have not been definitively identified. Melanopsin ( Opn4 ) is a novel opsin involved in of rhythms mammals. Here, we report cDNA cloning chicken melanopsin show its expression retina, brain gland. Like melanopsins identified amphibians mammals, more similar to invertebrate retinaldehyde‐based photopigments than typically found...
Background Previous lipidomic analyses of the human meibum had largely focused on individuals from non-Asian populations, despite higher prevalence dysfunctional tear syndrome (DTS) observed across Asia. Information pertaining to alterations in lipid profiles relation DTS onset and progression is also lacking warrants comprehensive experimental analysis. Methodologies/Principal Findings We examined lipidome 27 patients 10 control subjects for a total 256 species 12 major classes, including...
Diabetic retinopathy (DR) is a retinal microvascular disease characterized by inflammatory and angiogenic pathways. In this study, we evaluated NLRP3 inflammasome in double transgenic mouse model, Akimba (Ins2
To compare the early corneal wound repair and inflammatory responses after refractive lenticule extraction (ReLEx) LASIK.Eighteen rabbits underwent ReLEx another 18 LASIK. Each group was divided into three subgroups of six each these were subjected to corrections -3.00 diopters (D), -6.00 D, -9.00 D. Slit lamp photography, anterior segment optical coherence tomography (AS-OCT), topography, in vivo confocal microscopy performed 1 day surgery. After euthanatization, corneas immunofluorescent...
We compared early corneal nerve changes after small incision lenticule extraction (SMILE) and laser in situ keratomileusis (LASIK).A total of 12 rabbits underwent LASIK one eye SMILE the fellow eye. Baseline follow-up evaluations at 1, 2, 4 weeks postoperatively were performed with vivo confocal microscopy to evaluate 5 different areas within treated zone: center, superior, inferior, nasal, temporal. Cryosections corneas whole mount extracted lenticules analyzed immunostaining...
In this study, we elucidated the differential expression of a set local molecular factors in ectatic cone area cornea to uncover functional cause for focal corneal weakening characteristic keratoconus (KC) disease.All human samples were collected after approval Institutional Ethics Committee and informed consent. Keratoconus patients classified based on clinical parameters, topographical features, structural deformity. Epithelial cells from KC (n = 66) undergoing cross-linking procedures...
Abstract Background Vascular endothelial growth factor (VEGF)-induced angiogenesis is a critical compensatory response to microvascular rarefaction in the diabetic retina that contributes proliferative retinopathy (PDR). In this study, we sought determine role of specific micro ribonucleic acids (RNAs) (miRs) associated with VEGF patients PDR pathology. Methods RNA sequencing was employed detect differentially circulating miR diabetes mellitus (DM), nonproliferative (NPDR) and PDR....
Recent work has demonstrated that some actively transcribed genes closely associate with nuclear pore complexes (NPC) at the periphery. The Saccharomyces cerevisiae Mlp1 and Mlp2 proteins are components of inner basket mediate interactions these active genes. To investigate physical link between NPC loci, we identified interact carboxyl-terminal globular domain by tandem affinity purification coupled mass spectrometry. This analysis led to identification several...
PURPOSE. To study screening methods and associated factors of Meibomian gland disease dry eye subtypes in a specialized clinic Singapore. METHODS. This cross-sectional involved 200 patients clinic. The outcome measures evaluated were dysfunctional tear syndrome (DTS) level, meibomian grade, Schirmer test (ST) result, fluorescein break-up time (TBUT), corneal staining irritative symptoms. RESULTS. grade was with TBUT (P = 0.007), especially the upper eyelid correlated reading difficulty...
Purpose.: To investigate the potential of refractive lenticule (RL) storage and re-implantation in vivo as a method for reversing RL extraction (ReLEx) restoring corneal stromal volume. Methods.: ReLEx [−6.00 diopter (D) correction] was performed on six New Zealand White rabbits one eye. Each extracted tagged orientated before at −80°C 28 days. then re-implanted autologously correct orientation after flap relifting. All animals were monitored days being euthanized immunohistochemical...
Purpose.: To characterize the clinical profile of femtosecond lenticule extraction (FLEx) correlated with ultrastructural analysis corneal interface and in vivo real-time intraocular pressure (IOP). Methods.: Prospective case series experimental studies; consecutive patients underwent FLEx at a single tertiary center over 10 months postsurgical follow-up 3 months. The were divided into three groups according to spherical equivalence (SE) (A, < −5.0 diopters [D]; B, ≥ −5.00 D −9.00 D; C, −9.0...
Atropine, a muscarinic antagonist, is known to inhibit myopia progression in several animal models and humans. However, the mode of action not established yet. In this study, we compared quantitative iTRAQ proteomic analysis retinas collected from control lens-induced myopic (LIM) mouse eyes treated with atropine. The group received (−15D) spectacle lens over right eye on postnatal day 10 or without atropine drops starting 24. Axial length was measured by optical low coherence interferometry...
Purpose: We tested the potential of bone morphogenic protein 7 (BMP7) and hepatocyte growth factor (HGF) combination gene therapy to treat preformed corneal fibrosis using established rabbit in vivo human vitro models. Methods: Eighteen New Zealand White rabbits were used. Corneal was produced by alkali injury. Twenty-four hours after scar formation, cornea received topically either balanced salt solution (BSS; n = 6), polyethylenimine-conjugated gold nanoparticle (PEI2-GNP)-naked plasmid (n...