A5084138611- Parathyroid Disorders and Treatments
- RNA modifications and cancer
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Polyamine Metabolism and Applications
- Protein Kinase Regulation and GTPase Signaling
- Cancer-related molecular mechanisms research
- Erythrocyte Function and Pathophysiology
- Metabolism, Diabetes, and Cancer
- Ion channel regulation and function
- Molecular Biology Techniques and Applications
- CRISPR and Genetic Engineering
- Pancreatic function and diabetes
- Bacteriophages and microbial interactions
- Cancer-related gene regulation
- Amino Acid Enzymes and Metabolism
- Metallurgy and Material Forming
- Advanced biosensing and bioanalysis techniques
- Caveolin-1 and cellular processes
- Ion Transport and Channel Regulation
- Magnesium in Health and Disease
- Medical Imaging and Pathology Studies
- Genetic Syndromes and Imprinting
- bioluminescence and chemiluminescence research
- Bone Metabolism and Diseases
Biosearch Technologies (United States)
2014-2024
John Innes Centre
2023
Umeå University
2023
Uppsala University
1989-2022
Baylor College of Medicine
2013
University of Helsinki
2012
General Electric (Spain)
2011
Swedish Space Corporation
2009
Pediatrics and Genetics
2000
University of Colorado Boulder
1997-2000
The polyamines, putrescine, spermidine, and spermine, are essential polycations, intimately involved in the regulation of cellular proliferation. Although polyamines exert dynamic effects on conformation nucleic acids macromolecular synthesis vitro, their specific functions vivo poorly understood. We investigated function by overexpression a key catabolic enzyme, spermidine/spermine N(1)-acetyltransferase 1 (SAT1) mammalian cells. Transient cotransfection HeLa cells with GFP SAT1 vectors...
Hemoglobin synthesis in red cells is the major iron utilization pathway human body and accounts for > 80% of systemic turnover. The first step erythroid heme biosynthesis catalyzed by a tissue-specific isoform 5-aminolevulinate synthase (ALAS). previous identification iron-responsive elements 5'-untranslated region murine ALAS mRNA raised intriguing possibility that eALAS expression might be under iron-dependent translational control. As consequence, single post-transcriptional regulatory...
The multifaceted control of gene expression requires tight coordination regulatory mechanisms at transcriptional and post-transcriptional level. Here, we studied the interdependence transcription initiation, splicing polyadenylation events on single mRNA molecules by full-length sequencing. In MCF-7 breast cancer cells, find 2700 genes with interdependent alternative events, both in proximal distant parts molecules, including examples coupling between start sites sites. analysis three human...
Spinal muscular atrophy (SMA) is a neurodegenerative disease characterized by progressive motor neuron loss and caused mutations in SMN1 (Survival Motor Neuron 1). The severity inversely correlates with the copy number of SMN2, duplicated gene that nearly identical to SMN1. We have delineated mechanism transcriptional regulation SMN2 locus. A previously uncharacterized long noncoding RNA (lncRNA), SMN-antisense 1 (SMN-AS1), represses expression recruiting Polycomb Repressive Complex 2 (PRC2)...
The phylogenetically conserved eukaryotic translation initiation factor 5A (eIF5A) is the only known cellular protein to contain post‐translationally derived amino acid hypusine [ N ε ‐(4‐amino‐2‐hydroxybutyl)lysine]. Both eIF5A and its modification are essential for sustained cell proliferation. Normally one expressed in human cells. Recently, we identified a second EIF5A gene that would encode an isoform (eIF5A‐2) of 84% sequence identity. Overexpression eIF5A‐2 mRNA certain cancer cells,...
Eukaryotic translation initiation factor 5A (eIF5A) is the only cellular protein that contains unusual amino acid hypusine [N(epsilon)-(4-amino-2-hydroxybutyl)lysine]. Vertebrates carry two genes encode eIF5A isoforms, eIF5A-1 and eIF5A-2, which, in humans, are 84% identical. mRNA (1.3 kb) (18 kDa) constitutively expressed human cells. In contrast, expression of eIF5A-2 (0.7-5.6 (20 varies widely. Whereas was demonstrable most cells, detectable colorectal ovarian cancer-derived cell lines...
Sweden has an ambitious plan to fully decarbonise district heating by 2030 and contribute with negative emissions of greenhouse gases in 2050. The vagaries the energy market associated climate, political, social changes entail cross-sectoral integration that can fulfill these national targets. Fifth-generation cooling (5GDHC) is a relatively new concept systems features simultaneous supply using power-to-heat technologies. This paper presents best practices for developing 5GDHC reach...
Most mutations in the sequence of RNA hairpin that specifically binds MS2 coat protein either reduce binding affinity or have no effect. However, one mutation, a uracil to cytosine change loop, has unusual property increasing by nearly 100-fold. Guided structure protein–RNA complex, we used series and modifications evaluate thermodynamic basis for improved affinity: The tight mutation is due ( i ) amino group residue making an intra-RNA hydrogen bond increases propensity free adopt seen...
Four monoclonal anti-parathyroid antibodies were generated after immunization of mice with intact cells from human parathyroid tissue. All four reacted in immunohistochemistry structures present on epithelial and proximal-tubule the kidney but unreactive all other tissues investigated. Immunofluorescence microscopy suspended showed that cell surface. Two these efficiently blocked increase cytoplasmic calcium concentration is normally associated increased concentrations extracellular calcium....
The eukaryotic translation initiation factor 5A (eIF5A) is the only protein that contains hypusine [ N ε ‐(4‐amino‐2‐hydroxybutyl)lysine], which required for its activity. Hypusine formed by post‐translational modification of one specific lysine (Lys50 human eIF5A) deoxyhypusine synthase and hydroxylase. To investigate features eIF5A activity, we generated 49 mutations in eIF5A‐1, with a single amino acid substitution at highly conserved residues or N‐terminal C‐terminal truncations, tested...
Bacterial elongation factor P (EF-P) is the ortholog of archaeal and eukaryotic initiation 5A (eIF5A). EF-P shares sequence homology crystal structure with eIF5A, but unlike does not undergo hypusine modification. Recently, two bacterial genes, yjeA yjeK, encoding truncated homologs class II lysyl-tRNA synthetase lysine-2,3-aminomutase, respectively, have been implicated in modification to convert a specific lysine hypothetical β-lysyl-lysine. Here we present biochemical evidence for...
Nuclear receptors (NRs) are central regulators of pathophysiological processes; however, how their responses intertwine is still not fully understood.The aim this study was to determine whether and steroid NRs can influence each other's activity under co-agonist treatment.We used a unique system consisting multicopy integration an estrogen receptor responsive unit that allows direct visualization quantification alpha (ERa) DNA binding, co-regulator recruitment transcriptional readout.We find...
Cell cycle is a cellular process that subject to stringent control. In contrast the wealth of knowledge proteins controlling cell cycle, very little known about molecular role lncRNAs (long noncoding RNAs) in cell-cycle progression. By performing genome-wide transcriptome analyses cell-cycle-synchronized cells, we observed phase-specific induction >2000 lncRNAs. Further, demonstrate an S-phase-upregulated lncRNA, SUNO1 , facilitates progression by promoting YAP1-mediated gene expression....
Genomic SELEX is a method for studying the network of nucleic acid–protein interactions within any organism. Here we report discovery several interesting and potentially biologically important using genomic SELEX. We have found that bacteriophage MS2 coat protein binds Escherichia coli mRNA fragments more tightly than it natural, well-studied, phage site. from rffG (involved in formation lipopolysaccharide bacterial outer membrane), ebgR (lactose utilization repressor), as well other genes....
The expression of lipoprotein lipase (LPL) mRNA and the LPL activity were studied in macrophages (CD14 positive) from human atherosclerotic tissue. Macrophages isolated after collagenase digestion by immunomagnetic isolation. About 90% cells foam with oil red O positive lipid droplets. To analyze expression, PCR specific primers for was used. Arterial analyzed directly isolation data showed low when compared monocyte-derived macrophages. induce macrophages, PMA When incubating arterial 24 h...
Systemic lupus erythematosus (SLE) is a chronic autoimmune disease characterized by the production of anti-nuclear antibodies. SLE one many disorders that have strong gender bias, with 70-90% patients being female. Several explanations been postulated to account for severity diseases in females, including hormonal, microbiota, and gene dosage differences. X-linked TLRs recently implicated progression females. Our previous studies using 564Igi mouse model on Tlr7 Tlr9 double knockout...
ABSTRACT Hypusination is an essential posttranslational modification unique to archaeal and eukaryotic protein synthesis initiation factor 5A (aIF5A eIF5A, respectively). We have investigated the effect of efficient hypusination inhibitor N 1 -guanyl-1,7-diaminoheptane (GC 7 ) on four one bacterial species. found that (i) archaea are sensitive GC , whereas bacterium Escherichia coli not, (ii) causes rapid reversible arrest growth archaeon Sulfolobus acidocaldarius (iii) accompanied by a...
We describe a novel experimental approach to investigate mRNA translation. Antisense 2′-O-allyl oligoribonucleotides (oligos) efficiently arrest translation of targeted mRNAs in rabbit reticulocyte lysate and wheat germ extract while displaying minimal non-specific effects on Oligo/mRNAhybrids positioned anywhere within the 5′ UTR or first ∼20 nucleotides open reading frame block cap-dependent initiation with high specificity. The thermodynamic stability hybrids between 2′-O-alkyl oligos RNA...