A5102882886- Cancer therapeutics and mechanisms
- Acute Lymphoblastic Leukemia research
- Peptidase Inhibition and Analysis
- Cancer, Hypoxia, and Metabolism
- Glioma Diagnosis and Treatment
- Acute Myeloid Leukemia Research
- Ubiquitin and proteasome pathways
- Cancer Research and Treatments
- Cancer Treatment and Pharmacology
- Drug Transport and Resistance Mechanisms
- Computational Drug Discovery Methods
- Parathyroid Disorders and Treatments
- 3D Printing in Biomedical Research
- Cancer-related Molecular Pathways
- Chronic Myeloid Leukemia Treatments
- Metabolism, Diabetes, and Cancer
- Pancreatic function and diabetes
- Chronic Lymphocytic Leukemia Research
- Histone Deacetylase Inhibitors Research
- Cancer Cells and Metastasis
- Pharmacogenetics and Drug Metabolism
- Lung Cancer Research Studies
- Epigenetics and DNA Methylation
- Neuroblastoma Research and Treatments
- Colorectal Cancer Treatments and Studies
Uppsala University
2016-2025
Science for Life Laboratory
2022-2023
Uppsala University Hospital
2010-2022
Corline Systems (Sweden)
2008-2017
Karolinska Institutet
2013-2014
University of Oslo
2006-2008
KTH Royal Institute of Technology
1982-2007
AlbaNova
2007
University Hospital of North Norway
2006
Linnaeus University
2006
Abstract Background Although aberrant DNA methylation has been observed previously in acute lymphoblastic leukemia (ALL), the patterns of differential have not comprehensively determined all subtypes ALL on a genome-wide scale. The relationship between methylation, cytogenetic background, drug resistance and relapse is poorly understood. Results We surveyed levels 435,941 CpG sites samples from 764 children at diagnosis 27 relapse. This survey uncovered four characteristic signatures. First,...
Abstract Abnormal vascularization of solid tumours results in the development microenvironments deprived oxygen and nutrients that harbour slowly growing metabolically stressed cells. Such cells display enhanced resistance to standard chemotherapeutic agents repopulate after therapy. Here we identify small molecule VLX600 as a drug is preferentially active against quiescent colon cancer 3-D microtissues. The anticancer activity associated with reduced mitochondrial respiration, leading...
Intratumoral heterogeneity is a hallmark of glioblastoma multiforme and thought to negatively affect treatment efficacy. Here, we establish libraries glioma-initiating cell (GIC) clones from patient samples find extensive molecular phenotypic variability among clones, including range responses radiation drugs. This widespread was observed as continuum multitherapy resistance phenotypes linked proneural-mesenchymal shift in the transcriptome. Multitherapy associated with semi-stable state...
A thrombotic/inflammatory reaction is elicited when isolated islets of Langerhans come in contact with ABO-compatible blood. The detrimental effects this instant blood-mediated inflammatory (IBMIR) provide a reasonable explanation for the observation that an unexpectedly high number islets, from several donors, are needed to produce normoglycemia transplant patients type 1 diabetes. In study, hypothesis specific thrombin inhibitor, Melagatran, could reduce IBMIR vitro model which human...
Abstract An automated fluorometric microculture cytotoxicity assay (FMCA) based on the measurement of fluorescence generated from cellular hydrolysis fluorescein diacetate (FDA) to was employed for chemotherapeutic‐drug‐sensitivity testing tumor‐cell suspensions patients with leukemia. Fluorescence linearly related cell number, and reproducible measurements drug sensitivity could be performed using fresh or cryopreserved leukemia cells. A marked heterogeneity respect chemotherapeutic...
Background. Porcine islets offer an attractive alternative to human in clinical islet transplantation. The preferred method of transplantation is intraportal injection into the liver. We have recently shown, both vitro with and vivo porcine islets, that exposed allogeneic blood trigger injurious inflammatory reaction characterized by activation coagulation complement systems. now tested whether a similar triggered when xenogeneic are after primates. Furthermore, we investigated effect...
A crude fraction of Viola tricolor rich in small lipophilic proteins was prepared and subjected to fractionation guided by bioactivity, using RP-HPLC a fluorometric cytotoxicity assay. Two human cancer cell lines, U-937 GTB (lymphoma) RPMI-8226/s (myeloma), were used this study. The most potent compounds isolated, that is, the showing lowest IC50 values, shown be three proteins: vitri (IC50 = 0.6 μM 1 μM, respectively), varv 6 3 E 4 both lines). Their sequences, determined automated Edman...
Abstract Because dormant cancer cells in hypoxic and nutrient-deprived regions of solid tumors provide a major obstacle to treatment, compounds targeting those might have clinical benefits. Here, we describe high-throughput drug screening approach, using glucose-deprived multicellular tumor spheroids (MCTS) with inner hypoxia, identify that specifically target this cell population. We used concept repositioning—using known molecules for new indications. This is promising strategy rapid...
Abstract Background Environment inside even a small tumor is characterized by total (anoxia) or partial oxygen deprivation, (hypoxia). It has been shown that radiotherapy and some conventional chemotherapies may be less effective in hypoxia, therefore it important to investigate how different drugs act microenvironments. In this study we perform large screening of the effects 19 clinically used experimental chemotherapeutic on five cell lines conditions normoxia, hypoxia anoxia. Methods A...
In the present study, we screened a compound library containing 1,600 clinically used compounds with aim to identify compounds, which potentially could be repositioned for colon cancer therapy.Two established cell lines were tested using fluorometric microculture cytotoxicity assay (FMCA). For comparison connectivity map (CMAP) analysis, NCI 60 data mining and protein kinase binding measurements performed.Sixty-eight defined as hits activity in both of these (<40 % survival compared control)...
Abstract Hedgehog (HH) signaling is an important regulator of embryogenesis that has been associated with the development several types cancer. HH characterized by Smoothened (SMO)‐dependent activation GLI transcription factors, which regulate expression critical developmental genes. Neuroblastoma, embryonal tumor sympathetic nervous system, was recently shown to express high levels key molecules in this cascade. Using compounds blocking SMO (cyclopamine and SANT1) or GLI1/GLI2 (GANT61)...
Summary Titanium has superior osteointegrating properties compared to other biomaterials. The mechanism for this is unknown. During the initial phase of bone implantation biomaterial comes into direct contact with whole blood. In study we use a newly developed in vitro chamber model compare different commonly used biomaterials These materials were selected respect their order correlate these response presence 3 IU/ml heparin only titanium induced macroscopic clotting. This was reflected by...
Abstract Contact between blood and a biomaterial surface induces an immediate complement-mediated inflammatory response. Under these conditions, the alternative pathway of complement is often initiated amplified on surface. Adsorption protein such as C3 to polymer conformational changes in protein. Based expression adsorbed neoepitopes specific for bound fragments, we have hypothesized that able bind factor B form functional C3,Bb convertase. Using quartz crystal microbalance monitor binding...
The proteasome pathway is an important target for anticancer drug development. Here, we identify the antialcoholism disulfiram and its analogue pyrrolidine dithiocarbamate (PDTC) as inhibitors of 26S activity in a cell-based screening assay. As expected inhibitors, these compounds also inhibited TNF-alpha-induced nuclear factor-KappaB (NF-KappaB) translocation were cytotoxic. Disulfiram was more cytotoxic against chronic lymphocytic leukemia cells compared to peripheral blood mononuclear...