A5009728257- Retinal Diseases and Treatments
- Retinal Development and Disorders
- MicroRNA in disease regulation
- Retinal Imaging and Analysis
- Polyamine Metabolism and Applications
- Immune Cell Function and Interaction
- HIV Research and Treatment
- Retinopathy of Prematurity Studies
- Retinoids in leukemia and cellular processes
- Glaucoma and retinal disorders
- Estrogen and related hormone effects
- Extracellular vesicles in disease
- Cytomegalovirus and herpesvirus research
- CRISPR and Genetic Engineering
- Cancer-related gene regulation
- Retinal and Optic Conditions
- melanin and skin pigmentation
- RNA and protein synthesis mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Amino Acid Enzymes and Metabolism
- Neuroinflammation and Neurodegeneration Mechanisms
- HIV/AIDS Research and Interventions
- Adenosine and Purinergic Signaling
- Bacteriophages and microbial interactions
- Cardiovascular Disease and Adiposity
University of California, Davis
2015-2024
UC Davis Health System
2021
Indiana University School of Medicine
2014
University of California Davis Medical Center
2014
Office of Infectious Diseases
1998
National Institute of Dental and Craniofacial Research
1991
National Institutes of Health
1991
University of California, Irvine
1988
Translation intitiation factor eIF-5A (previously named eIF-4D) is a highly conserved protein that promotes formation of the first peptide bond. One its lysine residues modified by spermidine to form hypusine, posttranslational modification unique eIF-5A. To elucidate function and determine role hypusine modification, cDNA encoding human was used as probe identify clone corresponding genes from yeast Saccharomyces cerevisiae. Two TIF51A TIF51B were cloned sequenced. The two proteins are...
ABSTRACT Gastrointestinal complications in human immunodeficiency virus (HIV) infection are indicative of impaired intestinal mucosal immune system. We used simian (SIV)-infected rhesus macaques as an animal model for HIV to determine pathogenic effects SIV on T lymphocytes. Intestinal CD4 + T-cell depletion and the potential cytokine responses were examined during compared with results lymphocytes from lymph nodes blood. Flow cytometric analysis demonstrated severe CD8 − single-positive...
Translation intitiation factor eIF-5A (previously named eIF-4D) is a highly conserved protein that promotes formation of the first peptide bond. One its lysine residues modified by spermidine to form hypusine, posttranslational modification unique eIF-5A. To elucidate function and determine role hypusine modification, cDNA encoding human was used as probe identify clone corresponding genes from yeast Saccharomyces cerevisiae. Two TIF51A TIF51B were cloned sequenced. The two proteins are...
The phylogenetically conserved eukaryotic translation initiation factor 5A (eIF5A) is the only known cellular protein to contain post‐translationally derived amino acid hypusine [ N ε ‐(4‐amino‐2‐hydroxybutyl)lysine]. Both eIF5A and its modification are essential for sustained cell proliferation. Normally one expressed in human cells. Recently, we identified a second EIF5A gene that would encode an isoform (eIF5A‐2) of 84% sequence identity. Overexpression eIF5A‐2 mRNA certain cancer cells,...
ABSTRACT Intraepithelial lymphocytes (IEL) are a critical effector component of the gut-associated lymphoid tissue (GALT) and play an important role in mucosal immunity as well maintenance epithelial cell integrity barrier function. The objective this study was to determine whether simian immunodeficiency virus (SIV) infection rhesus macaques would cause alterations immunophenotypic profiles IEL their mitogen-specific cytokine (gamma interferon [IFN-γ] MIP-1β) responses (by flow cytometry)...
Protein synthesis initiation factor 4D (eIF-4D) from mammalian cells contains the post-translationally modified lysine derivative hypusine. A highly purified preparation of protein rabbit reticulocytes was subjected to chemical and enzymatic cleavage, a large number overlapping peptides were resolved by high performance liquid chromatography sequenced. Two mixed 14-base DNA probes synthesized based on suitable amino acid sequences used screen human cDNA library in lambda gt11. insert...
Dysregulation of protein synthesis has been implicated in oncogenesis through a mechanism whereby "weak" mRNAs encoding proteins involved cell proliferation are strongly translated when the apparatus is activated. Previous work determined that many cancer cells contain high levels eIF3h, subunit translation initiation factor eIF3, and overexpression eIF3h malignantly transforms immortal NIH-3T3 cells. This general feature as also affect translation, proliferation, number malignant phenotypes...
Purpose: To employ type II clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 endonuclease to suppress ocular angiogenesis by genomic disruption of VEGF-A in human RPE cells. Methods: CRISPR sequences targeting exon 1 were computationally identified based on predicted Cas9 on- and off-target probabilities. Single guide RNA (gRNA) cassettes with these target cloned into lentiviral vectors encoding the Streptococcus pyogenes (SpCas9) gene. The used infect ARPE-19 cells, a...
Protein synthesis initiation factor eIF-4D is a relatively abundant protein in mammalian cells and possesses unique amino acid residue, hypusine. The role of the hypusine modification function was addressed by studying variants lacking cloned human cDNA encoding overexpressed Escherichia coli precursor form purified. This fails to stimulate methionyl-puromycin vitro, nor does it significantly inhibit action native eIF-4D. Mammalian expression vectors were constructed with wild-type mutant...
Eukaryotic protein synthesis initiation factor 4D (eIF-4D) (current nomenclature, eIF-5A) contains the unique amino acid hypusine (N epsilon-(4-amino-2-hydroxybutyl)lysine). The first step in biosynthesis, i.e. formation of intermediate, deoxyhypusine epsilon-(4-aminobutyl)lysine), was carried out vitro using spermidine, synthase, and ec-eIF-4D(Lys), an eIF-4D precursor prepared by over-expression human cDNA Escherichia coli. In a parallel reaction, N-(3-aminopropyl)cadaverine place variant...
The nuclear hormone receptor family of transcription factors regulates gene expression via a complex combinatorial network interactions. Of particular interest is the ability retinoid X receptors (RXRs) to form heterodimers with retinoic acid (RARs) and thyroid (TRs), thereby modifying their activities. We report here that RXR, RAR, TR function can be reconstituted in yeast Saccharomyces cerevisiae demonstrate regulation seen vertebrate cells reproduced background. Using this system, we have...
Intravitreal murine lineage-negative bone marrow (BM) hematopoietic cells slow down retinal degeneration. Because human BM CD34+ are not precisely comparable to cells, this study examined the effect of intravitreal on degenerating retina using a model.C3H/HeJrd1/rd1 mice, immunosuppressed systemically with tacrolimus and rapamycin, were injected intravitreally PBS (n = 16) or isolated from magnetic cell sorter labeled enhanced green fluorescent protein (EGFP). After 1 4 weeks, eyes imaged...
The erb A oncogene is a dominant negative allele of thyroid hormone receptor gene and acts in the cancer cell by encoding transcriptional repressor. We demonstrate here that DNA sequence recognition properties oncogenic form protein are significantly altered from those normal receptors more closely resemble retinoic acid receptors; this alteration appears to play an important role defining targets action neoplasia. Unexpectedly, novel encoded N-terminal region not previously implicated as...
The main objective of this pilot study was to identify circulatory microRNAs in aqueous or plasma that were reflecting changes vitreous diabetic retinopathy patients. Aqueous, and samples collected from a total 27 patients undergoing vitreoretinal surgery: 11 controls (macular pucker macular hole patients) 16 with diabetes mellitus(DM): DM-Type I proliferative retinopathy(PDR) (DMI-PDR), DM Type II PDR(DMII-PDR) nonproliferative DR(DMII-NPDR). MicroRNAs isolated using Qiagen microRNeasy kit,...
Background/Objective: The Rs1 exon-1-del rat (Rs1KO) XLRS model shows normal retinal development until postnatal day 12 (P12) when small cystic spaces start to form in the inner nuclear layer. These enlarge rapidly, peak at P15, and then collapse by P19. Methods: We explored possible involvement of Kir4.1 Aqp4, principal retina channels for water movement homeostasis, along with Muller glia cells (MGCs), using semi-quantitative fluorescent immunohistochemistry P7, P9, P12, P30, Rs1KO WT...
Foveoschisis involves the pathologic splitting of retinal layers at fovea, which may occur congenitally in X-linked retinoschisis (XLRS) or as an acquired complication myopia. XLRS is attributed to functional loss adhesion protein retinoschisin 1 (RS1), but pathophysiology myopic foveoschisis unclear due lack animal models. Here, we characterized a novel nonhuman primate model through clinical examination and multimodal imaging followed by morphologic, cellular, transcriptional profiling...
ABSTRACT Using the simian immunodeficiency virus (SIV)-infected rhesus macaque model, we performed a longitudinal study to determine effect of antiretroviral therapy on phenotype and functional potential CD4 + T cells repopulating intestinal mucosa in human infection. Severe depletion CD8 occurred during primary SIV The majority these were activated memory phenotype. Phosphonate 9-[2-(phosphomethoxypropyl]adenine (PMPA) treatment led moderate suppression viral loads repopulation by...