A5012060913- Plant Pathogenic Bacteria Studies
- Phytoplasmas and Hemiptera pathogens
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Cocoa and Sweet Potato Agronomy
- Banana Cultivation and Research
- Legume Nitrogen Fixing Symbiosis
- Insect symbiosis and bacterial influences
- Insect-Plant Interactions and Control
- CRISPR and Genetic Engineering
- Plant Pathogens and Fungal Diseases
- Plant Parasitism and Resistance
- Coffee research and impacts
- Agricultural and Food Sciences
- Infections and bacterial resistance
- Plant tissue culture and regeneration
Agronomical Institute of Campinas
2019-2024
Instituto Agronômico do Paraná
2020
Anhanguera (Brazil)
2016-2020
IAC (United States)
2018-2020
Citrus cropping is widely distributed as an important economic activity worldwide. Amongst the major producers are Brazil, China, United States, Mexico and some European countries. Brazil largest sweet orange producer accounting for more than three-quarters of juice exports around world, followed by China States (Foreign Agricultural Service/USDA, 2019). Although main commodity in many countries producing citrus, by-products like essential oils, molasses, dried pulp, pectin, blend syrup...
Huanglongbing (HLB) is a destructive disease of citrus. The caused by the phloem-limited fastidious proteobacterium Candidatus Liberibacter asiaticus, which transmitted Asian citrus psyllid (Diaphorina citri). symptoms HLB have been related callose accumulation in phloem sieve plates. key class enzymes for synthesis Callose Synthases. synthase genes (calS) expression modulated biotic and abiotic stresses. In this study, nine C. sinensis calS (CscalS) were identified patterns analyzed CaLas...
RNA helicases are enzymes that catalyze the separation of double-stranded (dsRNA) using free energy ATP binding and hydrolysis. DEAD/DEAH families participate in many different aspects metabolism, including synthesis, folding, RNA-RNA interactions, localization degradation. Several important bacterial DEAD/DEAH-box have been extensively studied. In this study, we characterize ATP-dependent helicase encoded by hrpB (XAC0293) gene deletion genetic complementation assays. We provide insights...
Abstract Huanglongbing (HLB), caused mainly by ‘ Candidatus Liberibacter asiaticus’ (CLas), is the most devastating citrus disease because all commercial species are susceptible. HLB tolerance has been observed in Poncirus trifoliata and their hybrids. A wide-ranging transcriptomic analysis using contrasting genotypes regarding severity was performed to identify genetic mechanism associated with HLB. The included Citrus sinensis, sunki, three distinct groups of hybrids obtained from crosses...
Xanthomonas citri subsp. causes citrus canker disease worldwide in most commercial varieties of citrus. Its transmission occurs mainly by wind-driven rain. Once X. reaches a leaf, it can epiphytically survive forming biofilm, which enhances the persistence bacteria under different environmental stresses and plays an important role early stages host infection. Therefore, study genes involved biofilm formation has been step toward understanding bacterial strategy for survival infection plants....
Xanthomonas citri subsp. (X. citri) is a plant pathogenic bacterium causing citrus canker disease. The xanA gene encodes phosphoglucomutase/phosphomannomutase protein that key enzyme required for the synthesis of lipopolysaccharides and exopolysaccharides in Xanthomonads. In this work, firstly we isolated transposon mutant (xanA::Tn5) analyzed its phenotypes as biofilm formation, xanthan gum production, pathogenesis on sweet orange host. Moreover, to confirm role impaired further produced...
The emergence of citrus huanglongbing (HLB) has been a constraint for worldwide growers. HLB disease is associated to species the biothrophic bacteria Candidatus Liberibacter spp. (CaL). In this study, we assessed transcriptional status salicylic acid (SA) genes and defenses between two contrasting genotypes during challenge with Ca. asiaticus or americanus. Citrus sinensis exhibited most evident alterations in gene expression evaluated genes, when compared Poncirus trifoliata. Upstream...
Abstract Xanthomonas is an important genus of plant-associated bacteria that causes significant yield losses economically crops worldwide. Different approaches have assessed genetic diversity and evolutionary interrelationships among the species. However, information from clustered regularly interspaced short palindromic repeats (CRISPRs) has yet to be explored. In this work, we analyzed architecture CRISPR–Cas loci presented a sequence similarity-based clustering conserved Cas proteins in...
Parental plants, an F1 interspecific hybrid and the F2 generation from Arabusta coffee plants were investigated for meiotic behavior viability of pollen to understand part their reproductive biology that affects possible use in breeding programs. Both parental (C. canephora var. Robusta 4x C. arabica dihaploid Bourbon Vermelho) showed a meiosis diploid-like behavior, despite presenting small percentage irregularities, just as occurred hybrid. On other hand, all showeda higher frequency...
Citrus plants have been extremely affected by Huanglongbing (HLB) worldwide, causing economic losses. HLB disease causes disorders in citrus plants, leading to callose deposition the phloem vessel sieve plates. Callose is synthesized synthases, which are encoded 12 genes (calS1- calS12)in Arabidopsis thaliana. We evaluated expression of eight synthase from hybrids between sunki and Poncirus trifoliata infected with HLB. The objective this work was identify possible tolerance loci combining...
Xathomonas citri subsp. causes citrus canker disease worldwide in most commercial varieties of citrus. Its transmission occurs mainly through wind-driven rain. Once on the leaf X. can epiphytically survive forming biofilm, which enhances persistence bacteria to different environmental stresses and play an important role early stages host infection. Therefore, study genes involved biofilm formation has been step towards understanding bacterial strategy infect plant host. In this work we show...