A5025879797- Thyroid Cancer Diagnosis and Treatment
- Genetic factors in colorectal cancer
- Cancer-related Molecular Pathways
- Lung Cancer Treatments and Mutations
- Advanced Proteomics Techniques and Applications
- Plant tissue culture and regeneration
- S100 Proteins and Annexins
- Botany and Plant Ecology Studies
- Thyroid Disorders and Treatments
- Cancer Genomics and Diagnostics
- Cancer Immunotherapy and Biomarkers
- Colorectal Cancer Treatments and Studies
- Occupational and environmental lung diseases
- Plant Pathogens and Fungal Diseases
- BRCA gene mutations in cancer
- Seed Germination and Physiology
- Horticultural and Viticultural Research
- Plant Disease Management Techniques
- Plant and animal studies
- Biomedical Text Mining and Ontologies
- Plant and Fungal Interactions Research
- Nuts composition and effects
- RNA modifications and cancer
- Genetic diversity and population structure
- Forest ecology and management
University of Pisa
2015-2024
Azienda Ospedaliera Universitaria Pisana
2008-2023
Saint Louis University
2014
Ospedale di Livorno
2014
University of Chicago
2014
Mayo Clinic in Arizona
2014
University of Florence
2003-2010
Istituto di Genetica Molecolare
1995-2007
National Academies of Sciences, Engineering, and Medicine
2003-2004
Istituto Nazionale di Fisica Nucleare, Sezione di Pisa
2004
Abstract Activating point mutations of the BRAF gene have been recently reported in papillary thyroid carcinomas. In this study, we analyzed 320 tumors and six anaplastic carcinoma cell lines detected 45 (38%) carcinomas, two (13%) poorly-differentiated three (10%) five (83%) but not follicular, Hürthle cell, medullary follicular adenomas, or benign hyperplastic nodules. All involved a T→A transversion at nucleotide 1796. were associated with older age, classic tall variant histology,...
Background: The BRAFV600E mutation is the most frequent genetic alteration in papillary thyroid carcinoma (PTC). role of as a poor prognostic factor has been controversially reported series with short-term follow-ups. In this study we verified value PTC patients long-term follow-up. Methods: We studied 102 median follow-up 15 yr. was analyzed by PCR-single-strand conformational polymorphism and sequencing. correlation between presence/absence mutation, clinicopathological features, outcome...
Because very few studies have examined the correlation between BRAF mutations and clinicopathological features of papillary thyroid carcinoma (PTC), we analyzed here a large homogeneous cohort patients with PTC for presence mutation.We in consecutive series 500 who underwent surgery Department Surgery University Pisa, correlated mutation parameters patients: age, gender, tumor size, capsule, extrathyroidal invasion, multicentricity, node metastases, class.BRAF (exon 15) was by PCR-single...
The BRAF(V600E) mutation, the most frequent genetic alteration in papillary thyroid carcinoma (PTC), was demonstrated to be a poor prognostic factor. aim of this study evaluate its significance large cohort low-risk intrathyroid PTC.Among 431 consecutive PTC patients, we selected 319 patients with an tumor and no metastases (T1-T2, N0, M0). mutation analyzed by PCR-single-strand conformation polymorphism analysis direct genomic sequencing. correlation between presence/absence...
Individualized management, incorporating papillary thyroid cancer (PTC) variant-specific risk, is conceivably a useful treatment strategy for PTC, which awaits comprehensive data demonstrating differential risks of PTC variants to support.This study sought establish the clinicopathological risk major variants: conventional (CPTC), follicular-variant (FVPTC), and tall-cell (TCPTC).This was retrospective outcomes 6282 patients (4799 females 1483 males) from 26 centers The Cancer Genome Atlas...
Point mutations in BRAF are genetic hallmarks of papillary thyroid carcinoma (PTC). In this retrospective study, we examined aspirates and corresponding paraffin-embedded surgical samples for the presence mutations. Altogether, 96 cases, including 69 PTCs, 19 follicular adenomas, eight nontoxic nodular goiters BRAF; 60 these were also RET/PTC rearrangements. The results correlated with cytological diagnosis final histopathology. mutation (V599E) was detected 38% that PTC on histopathology;...
In papillary thyroid carcinomas (PTCs), rearrangements of the RET receptor (RET/PTC) and activating mutations in BRAF or RAS oncogenes are mutually exclusive. Here we show that 3 proteins function along a linear oncogenic signaling cascade which RET/PTC induces RAS-dependent activation RAS- BRAF-dependent ERK activation. Adoptive RET/PTC-RAS-BRAF axis induced cell proliferation Matrigel invasion follicular cells. Gene expression profiling revealed activate common transcriptional program...
Evaluation of the degree neoplastic infiltration beyond thyroid capsule remains a unique parameter that can be evaluated by histopathological examination to label papillary carcinoma (PTC) 20 mm or less in size as pT1 pT3 tumor.We correlated BRAF V600E mutation with both clinical-pathological features and redefine reliability actual system risk stratification large selected group PTCs smaller than mm.The presence mutations was examined 1060 divided into four degrees infiltration: 1) totally...
NRAS mutations occur in 3–5% of colorectal cancer. Differently from KRAS and BRAF mutations, the role as prognostic predictive markers metastatic cancer (mCRC) has been investigated to a lesser extent. A retrospective series suggested predictors resistance anti-EGFR monoclonal antibodies (MoAbs) chemo-refractory patients with mCRC. In our study, codons 12, 13, 61 codon 600 mutational status were evaluated mCRCs referred Institution 2009 2012. 13 was analyzed KRAS/BRAF wt patients. We...
The SARS-CoV-2 genome has been detected in a variety of human samples including blood, urine, semen, and faeces. However, evidence virus presence tissues other than lung are limited.We investigated whether could be 50 autoptic specimens endocrine organs from 29 patients who died COVID-19.The was 25 ten abdominal subcutaneous adipose tissue (62%), six testes (67%), nine thyroid (36%) samples. analysis multiple organ obtained the same showed that, virus-positive cases, viral consistently all...
Obesity is a recognized risk factor for the progression to severe forms of COVID-19, yet mechanisms association are unclear.Subcutaneous abdominal adipose tissue specimens subjects deceased from COVID-19 (n = 23) were compared those controls dying abruptly causes other than infectious (accidental trauma, sudden cardiac death). Alterations lung parenchyma consistent with moderate disease detected in all cases, not controls. Investigations included: histopathologic features, detection virus...
Papillary thyroid carcinoma (PTC) is frequently multifocal. Independent PTC foci may occur either from intraglandular metastases a single dominant tumor or as unrelated neoplastic clones. In rare cases, the simultaneous presence of different histopathological subtypes points to independent sites formation.We examined pattern BRAF mutations in noncontiguous and node 69 patients affected by multicentric PTC. These included 19 cases characterized variants.BRAF (exon 15) mutation was PCR-single...
Echinoderm microtubule associated proteinlike 4-anaplastic lymphoma receptor tyrosine kinase (EML4-ALK) translocation has been described in a subset of patients with non-small cell lung cancer (NSCLC) and shown to have oncogenic activity. Fluorescence situ hybridization (FISH) is used detect ALK-positive NSCLC, but it expensive, time-consuming, difficult for routine application.To evaluate the potential role immunohistochemistry (IHC) as screening tool identify candidate cases FISH analysis...
The understanding of the mechanisms underlying thyroid cancer immune escape can lead to identification new molecular targets and/or efficacy biomarkers. For this purpose, we performed expression profiling in cancers obtain a comprehensive view on activated during progression. study was conducted retrospectively 25 papillary carcinomas (PTCs), 14 poorly differentiated (PDTC), 13 anaplastic (ATCs), and 7 normal (NT) tissue samples. Gene obtained RNA samples using Nanostring platform its...
Abstract Context Glomerular hyperfiltration may represent a direct pathogenetic link between obesity and kidney disease. The most widely used methods to estimate creatine clearance such as Cockroft–Gault (CG), Modification of Diet in Renal Disease (MDRD), Chronic Kidney Epidemiology Collaboration (CKD-EPI) have not been validated subjects with obesity. Objective performance prediction formulas was compared measured creatinine (mCrCl) Methods study population included 342 patients (mean BMI...
In papillary thyroid carcinomas (PTCs), rearrangements of the RET receptor (RET/PTC) and activating mutations in BRAF or RAS oncogenes are mutually exclusive. Here we show that 3 proteins function along a linear oncogenic signaling cascade which RET/PTC induces RAS-dependent activation RAS- BRAF-dependent ERK activation. Adoptive RET/PTC-RAS-BRAF axis induced cell proliferation Matrigel invasion follicular cells. Gene expression profiling revealed activate common transcriptional program...
Thyroid ultrasound (US) elastography provides an estimation of tissue stiffness and is helpful to differentiate malignant from benign lesions. Tissue proprieties molecules causing are not established. The aim the study was correlate US findings with properties in thyroid nodules.A total 115 nodules 112 patients who underwent surgery for presence Thy 3 (indeterminate) cytology (n = 67), 4-5 (suspicious-indicative carcinoma) 47), or large goiter 2 1) suspicious features were examined by...
Background: Thyroid dysfunctions have been reported after Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection. However, the biological mechanisms behind these conditions remain unexplored. Herein, we report on changes of immune transcriptome in autoptic thyroid tissues people who died from coronavirus disease 2019 (COVID-19). Methods: Twenty-five specimens subjects dying COVID-19 were investigated. Eleven causes other than infectious served as controls. RNA transcripts 770...
Abstract Using an enrichment procedure, we cloned microsatellite repeats from European beech ( Fagus sylvatica L.) and developed primers for the amplification of markers. Six polymorphic loci were characterized which produced 3–21 alleles in 70 individuals one Italian population, with observed heterozygosity between 0.58 0.85. All six amplified fragments closely related species, orientalis, also. Owing to their very high degree variation, these markers should be useful gene flow studies species.
Context: The transmembrane glycoprotein CD44v6 is overexpressed in most papillary thyroid carcinomas (PTC). We previously reported that osteopontin (OPN), a secreted functions as ligand for CD44v6, thyrocytes transformed by the RET/PTC oncogene.
No study has evaluated the antiproliferative effects of thiazolidinediones and antiblastics in 'primary cultured human anaplastic thyroid cancer cells'.Primary cells proliferation was after incubation with increasing concentrations rosiglitazone or pioglitazone (bleomycin, cisplatin, gemcitabine) by a assay (WST-1-tetrazolium reaction) cell counting.A reduction at 1 h (from start tetrazolium [of 11% 25%, rosiglitazone, 10 20 (P = 0.0001) microM, respectively; 7% 17%, pioglitazone, 0.0125)...