A5062915879- Thyroid Disorders and Treatments
- Thyroid Cancer Diagnosis and Treatment
- Neuroendocrine Tumor Research Advances
- Metabolism, Diabetes, and Cancer
- Sirtuins and Resveratrol in Medicine
- Adenosine and Purinergic Signaling
- Tryptophan and brain disorders
- Neonatal Health and Biochemistry
- Cancer, Hypoxia, and Metabolism
- Ion Transport and Channel Regulation
- Virus-based gene therapy research
- Microplastics and Plastic Pollution
- Amino Acid Enzymes and Metabolism
- Neuroscience of respiration and sleep
- Selenium in Biological Systems
- Radiopharmaceutical Chemistry and Applications
- Ion channel regulation and function
- Extracellular vesicles in disease
- S100 Proteins and Annexins
- Zebrafish Biomedical Research Applications
- Glutathione Transferases and Polymorphisms
- Cancer Research and Treatments
- bioluminescence and chemiluminescence research
- Vitamin D Research Studies
- Effects and risks of endocrine disrupting chemicals
Instituto de Salud Carlos III
2015-2024
Unidad Funcional de Investigación de Enfermedades Crónicas
2015-2024
Centro Nacional de Microbiologia
2011-2023
Centro de Investigación Biomédica en Red de Cáncer
2021-2023
Sociedad Española de Endocrinología y Nutrición
2019-2020
Instituto de Investigaciones Biomédicas Sols-Morreale
1997-2010
Albert Einstein College of Medicine
1998-2009
Universidad Autónoma de Madrid
2009
Consejo Superior de Investigaciones Científicas
2009
Hospital Universitario La Paz
2009
Nanoplastics (NP) are present in aquatic and terrestrial ecosystems. Humans can be exposed to them through contaminated water, food, air, or personal care products. Mechanisms of NP toxicity largely unknown the Zebrafish embryo poses an ideal model investigate due its high homology with humans. Our objective study was combine a battery behavioral assays endocrine related gene expression, further explore potential neurotoxic effects on animal behavior. Polystyrene nanoplastics (PSNP) were...
Abstract The activating mutation BRAFV600E is a frequent genetic event in papillary thyroid carcinomas (PTC) that predicts poor prognosis, leading to loss of sodium/iodide symporter (NIS) expression and subsequent radioiodide-refractory metastatic disease. molecular basis such an aggressive behavior induced by BRAF remains unclear. Here, we show mechanism through which induces NIS repression promotes epithelial mesenchimal transition invasion based on the operation autocrine transforming...
The Na+/I−symporter (NIS), a 618-amino acid membrane glycoprotein that catalyzes the active accumulation of I− into thyroid cells, was identified and characterized at molecular level in our laboratory (Dai, G., Levy, O., Carrasco, N. (1996) Nature 379, 458–460). Because mature NIS is highly glycosylated, it migrates SDS-polyacrylamide gel electrophoresis as broad polypeptide higher mass (∼90–110 kDa) than nonglycosylated (∼50 kDa). Using site-directed mutagenesis, we substituted both...
The phenomenon that supraphysiological doses of iodide (I(-)) temporarily inhibit thyroid hormone synthesis is known as autoregulation. Recovery function has been attributed to sodium-iodide symporter (NIS) inhibition, but the diversity available data makes it difficult reach definitive conclusions. Iodide excess induces reactive oxygen species production and cell toxicity. However, roles oxidative state antioxidant selenoproteins in I(-) autoregulation have never explored. Here we analyze...
Zebrafish get their name from the black lines along bodies. These fish have many similarities with humans and help scientists investigate diseases. Sometimes diseases can be linked to our environment. Since started using plastic materials in last century, we been filling soil water garbage. With time, plastics break down become so tiny that they enter bodies of animals, including us. Scientists added one type these nanoplastics, called polystyrene, into where zebrafish eggs were developing....
The Na+/I– symporter (NIS) is a key plasma membrane glycoprotein that mediates Na+-dependent active I– transport in the thyroid, lactating breast, and other tissues. OH group of side chain at position 354 transmembrane segment (TMS) IX NIS has been demonstrated to be essential for function, as revealed by study congenital defect-causing T354P mutation. TMS most β-OH group-containing amino acids (Ser Thr) any NIS. We have thoroughly characterized functional significance all Ser Thr NIS, well...
Patients with congenital lack of I transport do not accumulate in their thyroids, often resulting severe hypothyroidism. A single amino acid substitution the thyroid Na+/I- symporter (NIS), proline replacing threonine at position 354 (T354P), was recently identified as cause this condition two independent patients. Here we report that I- activity T354P NIS generated by site-directed mutagenesis, is due to a structural change induced proline, but rather absence hydroxyl group beta-carbon...
Abstract The Na+/I− symporter (NIS) is a key membrane glycoprotein that mediates active I− transport in the thyroid and other tissues. Upon isolation of cDNA encoding NIS, 10 NIS mutations cause congenital iodide defect have been identified. Three these (T354P, G395R, Q267E) thoroughly characterized at molecular level. All three mutant proteins are correctly targeted to plasma membrane; however, whereas Q267E displays minimal activity, T354P G395R inactive. Here, we show contrast mutants,...
Coordination of gene expression with nutrient availability supports proliferation and homeostasis is shaped by protein acetylation. Yet how physiological/pathological signals link acetylation to specific programs whether such responses are cell-type–specific unclear. AMP-activated kinase (AMPK) a key energy sensor, activated glucose limitation resolve supply–demand imbalances, critical for diabetes cancer. Unexpectedly, we show here that, in gastrointestinal cancer cells, activates AMPK...
The Na(+)/I(-) symporter (NIS) is a key plasma membrane glycoprotein that mediates active iodide (I(-)) transport in the thyroid and other tissues. Since isolation of cDNA encoding NIS (G. Dai, O. Levy, N. Carrasco (1996) Nature 379, 458-460), ten mutations have been identified as causes congenital defect (ITD). Two these (T354P G395R) thoroughly characterized at molecular level. Both mutant proteins are inactive but normally expressed correctly targeted to membrane. hydroxyl group...
The sodium/iodide symporter (NIS) mediates a remarkably effective targeted radioiodide therapy in thyroid cancer; this approach is an emerging candidate for treating other cancers that express NIS, whether endogenously or by exogenous gene transfer. Thus far, the only extrathyroidal malignancy known to functional NIS breast cancer. Therapeutic efficacy cancer requires uptake be maximized tumor cells manipulating well-known regulatory factors of expression cells, such as TSH, which stimulates...