A5087675376- Phosphodiesterase function and regulation
- Heart Failure Treatment and Management
- Cardiac electrophysiology and arrhythmias
- Renin-Angiotensin System Studies
- Cardiac Fibrosis and Remodeling
- Pharmaceutical Economics and Policy
- Ion channel regulation and function
- Cardiac pacing and defibrillation studies
- Receptor Mechanisms and Signaling
- Adenosine and Purinergic Signaling
Université Paris-Saclay
2019-2025
Laboratory Signaling and Cardiovascular Pathophysiology
2019-2025
Inserm
2019-2025
Université Paris Cité
2022
Institut Galien Paris-Saclay
2019
The cyclic AMP (adenosine monophosphate; cAMP)-hydrolyzing protein PDE4B (phosphodiesterase 4B) is a key negative regulator of cardiac β-adrenergic receptor stimulation. deficiency leads to abnormal Ca2+ handling and decreased in pressure overload hypertrophy, suggesting that increasing the heart beneficial failure.We measured expression human tissues developed 2 transgenic mouse lines with cardiomyocyte-specific overexpression an adeno-associated virus serotype 9 encoding PDE4B. Myocardial...
PDE2 (phosphodiesterase 2) is upregulated in human heart failure. Cardiac PDE2-transgenic mice are protected against contractile dysfunction and arrhythmias failure but whether an acute elevation of could be therapeutic value remains elusive. This hypothesis was tested using cardiac gene transfer preclinical models C57BL/6 male were injected with serotype 9 adeno-associated viruses encoding for PDE2A. led to a ≈10-fold rise PDE2A protein levels that affected neither structure nor function...
New Findings What is the central question of this study? Can imipramine, an antidepressant agent that a cationic amphiphilic drug interferes with phosphatidylinositol 4,5‐bisphosphate (PI(4,5)P 2 ) interactions proteins maintaining tubular system, be validated as new detubulating tool? main finding and its importance? Imipramine was more efficient less toxic cardiomyocytes than formamide. insights are provided on how PI(4,5)P crucial to T‐tubule attachment cell surface cardiotoxic effects...
ABSTRACT BACKGROUND Constitutive cardiac PDE2 activation was shown to protect against contractile dysfunction and arrhythmia in heart failure (HF). However, it remains unknown whether an acute elevation of is efficient prevent maladaptive remodeling arrhythmia. In this study we tested increasing acutely PDE2A activity preclinical models HF using gene transfer could be therapeutic value. METHODS AND RESULTS C57BL/6 male mice were injected with serotype 9 adeno-associated viruses (AAV9)...