A5036721671- Connexins and lens biology
- Barrier Structure and Function Studies
- Ion channel regulation and function
- Mosquito-borne diseases and control
- Genetic and Kidney Cyst Diseases
- Cancer Research and Treatments
- Vibrio bacteria research studies
- Microtubule and mitosis dynamics
- Respiratory viral infections research
- Ion Transport and Channel Regulation
- Ion Channels and Receptors
- interferon and immune responses
- Pancreatic function and diabetes
- Hedgehog Signaling Pathway Studies
- Atherosclerosis and Cardiovascular Diseases
- Cellular transport and secretion
- Cardiac electrophysiology and arrhythmias
- Viral Infections and Outbreaks Research
- Dietary Effects on Health
- Parasitic Diseases Research and Treatment
- Hemoglobin structure and function
- Cell death mechanisms and regulation
- Drug Transport and Resistance Mechanisms
- Renal and related cancers
- Cannabis and Cannabinoid Research
Center for Research and Advanced Studies of the National Polytechnic Institute
2010-2023
Instituto Politécnico Nacional
1998-2023
Center for Neurosciences
2010
Instituto Nacional de Psiquiatría
2003
Tribunal Electoral del Estado de México
2002
Instituto de Medicina Tropical “Pedro Kourí”
1990-1994
Instituto de Medicina Tropical
1991-1994
Permeability alterations of microvascular endothelia may be a factor in the plasma leakage produced by dengue virus infection. Confluent monolayers human dermal endothelial cell line HMEC-1 were utilized as an experimental model to study cellular responses induced virus. Infected showed increased permeability for [(3)H]mannitol, but no changes observed 4-70 kDa dextrans at 48 h post-infection (p.i.), time which viral titres reached maximal values and 40 % cells expressed proteins. A further...
Epithelial cells treated with high concentrations of ouabain (e.g., 1 μM) retrieve molecules involved in cell contacts from the plasma membrane and detach one another their substrates. On basis this observation, we suggested that might also modulate at low, nontoxic levels (10 or 50 nM). To test possibility, analyzed its effect on a particular type cell–cell contact: tight junction (TJ). We demonstrate neither inhibit K + pumping nor disturb balance cell, modulates degree sealing TJ as...
Infection with dengue virus type-2 (DENV-2) begins adherence to cell surface receptors. In endothelial cells (HMEC-1), a model for DENV-2 infection, α 5 β 3 integrin has been identified as putative receptor the virus. Previous work had suggested that actin cytoskeleton of HMEC-1 plays an important role in entry and infection. present work, fixed living expressing enhanced green fluorescent protein–actin were monitored reorganization after inoculation, utilizing fluorescence time lapse...
Cannabidiol (CBD) has been used to treat a variety of cancers and inflammatory conditions with controversial results. In previous work, we have shown that breast cancer MCF-7 cells, selected by their response IL-1β cytokine, acquire malignant phenotype (6D cells) through an epithelial–mesenchymal transition (EMT). We evaluated CBD as potential inhibitor this inducer reversion non-invasive phenotype. It decreased 6D cell viability, downregulating expression receptor CB1. The blocked migration...
The exchange of substances between higher organisms and the environment occurs across transporting epithelia whose basic features are tight junctions (TJs) that seal intercellular space, polarity, which enables cells to transport vectorially. In a previous study, we demonstrated 10 nM ouabain modulates TJs, now show it controls polarity as well. We gauge through development cilium at apical domain Madin-Darby canine kidney (MDCK, epithelial dog kidney). Ouabain accelerates ciliogenesis in an...
The finding that endogenous ouabain acts as a hormone prompted efforts to elucidate its physiological function. In previous studies, we have shown 10 nM (i.e., concentration within the range) modulates cell-cell contacts such tight junctions and apical/basolateral polarity. this study, examined whether affects another important feature: gap junction communication (GJC).We employed two different approaches: 1) analysis of cell-to-cell diffusion neurobiotin injected into particular MDCK cell...
HEK293 cells are widely used as a host for expression of heterologous proteins; yet, little care has been taken to characterize their endogenous membrane components, including ion channels. In this work, we aimed describe the biophysical and pharmacological properties endogenous, voltage-dependent potassium currents (IKv). We also examined how its depends on culture conditions. electrophysiological technique whole-cell patch clamp record from cells. found that HEK express currents. diverse...
Inflammation and oxidative stress play major roles in endothelial dysfunction, are key factors the progression of cardiovascular diseases. The aim this study was to evaluate vitro effect three subfractions (SFs) from Cucumis sativus aqueous fraction reduce inflammatory induced by angiotensin II (Ang II) human microvascular cells-1 (HMEC-1) cells. cells were cultured with different concentrations Ang 0.08 or 10 μg/mL SF1, SF2, SF3, μmol losartan as a control. IL-6 (Interleukin 6)...
The fact that ouabain has been identified as an endogenous substance, led us to inquire its physiological role in epithelial cells. Based on previous observations, we hypothesized it influences processes related cell contacts. Previously have shown nanomolar concentrations of up-regulate tight junctions, accelerate ciliogenesis, and increase gap junctional intercellular communication (GJIC). Given silencing assays indicated connexin 43 (Cnx43) is involved the GJIC response, present work...
Ouabain, a well-known plant-derived toxin, is also hormone found in mammals at nanomolar levels that binds to site located the a-subunit of Na⁺,K⁺-ATPase. Our main goal was understand physiological roles ouabain. Previously, we ouabain increases degree tight junction sealing, GAP junction-mediated communication and ciliogenesis. Considering our previous results, investigated effect on adherens junctions.We used immunofluorescence immunoblot methods measure 10 nM cellular nuclear content...
Cardiac glycosides are a group of compounds widely known for their action in cardiac tissue, some which have been found to be endogenously produced (ECG). We previously studied the effect ouabain, an endogenous glycoside, on physiology epithelial cells, and we shown that concentrations nanomolar range, it affects key properties such as tight junction, apical basolateral polarization, gap junctional intercellular communication (GJIC), adherent junctions. In this work, study influence digoxin...
Ouabain is a cardiac glycoside, initially isolated from plants, and currently thought to be hormone since some mammals synthesize it endogenously. It has been shown that in epithelial cells, induces changes properties components related apical-basolateral polarity cell-cell contacts. In this work, we used whole-cell patch clamp test whether ouabain affects the of voltage-gated potassium currents (Ik) cells (MDCK). We found that: (1) arranged as mature monolayers, induced Ik; (2) also...
Abstract Little is known about the presence and distribution of tubulin isotypes in MDCK cells although essential epithelial functions these monolayers are regulated by dynamic changes microtubule architecture. Using specific antibodies, we show here that βI, βII, βIV differentially distributed microtubules cells. Microtubules subconfluent radiating from perinuclear region contain βI βII tubulins, while those extending to cell edges enriched βII. Confluent similar proportions along entire...
Prostaglandins are a group of lipids that produce diverse physiological and pathological effects. Among them, prostaglandin E2 (PGE2) stands out for the wide variety functions in which it participates. To date, there is little information about influence PGE2 on gap junctional intercellular communication (GJIC) any type tissue, including epithelia. In this work, we set to determine whether influences GJIC epithelial cells (MDCK cells). end, performed dye (Lucifer yellow) transfer assays...