Nanotechnology is expected to open new avenues fight and prevent disease using atomic scale tailoring of materials. Among the most promising nanomaterials with antibacterial properties are metallic nanoparticles, which exhibit increased chemical activity due their large surface volume ratios crystallographic structure. The study bactericidal particularly timely considering recent increase resistant strains bacteria potent antibiotics. This has promoted research in well known silver ions...

Expression of the type II voltage-dependent sodium channel gene is restricted to neurons by a silencer element active in nonneuronal cells. We have cloned cDNA coding for transcription factor (REST) that binds this element. recombinant REST protein confers ability silence reporter genes neuronal cell types lacking native protein, whereas expression dominant negative form cells relieves silencing mediated protein. transcripts developing mouse embryos are detected ubiquitously outside nervous...

The type II voltage-dependent sodium channel is present in neuronal cells, where it mediates the propagation of nerve impulses. Restricted expression gene to neurons due, at least part, binding repressor protein REST (also termed NRSF or XBR) RE1 called NRSE) sequence gene. Previous studies have shown that a domain containing eight GL1-Krüppel zinc finger motifs DNA binding. Deletional and GAL4-fusion analyses now reveal domains lie outside DNA-binding both amino carboxyl termini REST....

A green method for an efficient synthesis of water-soluble carbon nanoparticles (CNPs), graphitic shell encapsulated nanocubes (CNCs), Carbon dots (CDs) using Camphor (Cinnamomum camphora) is demonstrated. Here, we describe a competent molecular fusion and fission route step-wise CDs. on acidification carbonization forms CNPs, which alkaline hydrolysis form CNCs that are by thick layers further reduction sodium borohydride yielded Though excitation wavelength dependent photoluminescence...

Abstract A multifunctional magneto-plasmonic CoFe 2 O 4 @Au core-shell nanoparticle was developed by iterative-seeding based method. This nanocargo consists of a cobalt ferrite kernel as core (Nk) and multiple layers gold functionalizable active stratum, (named Nk@A after fifth iteration). helps in augmenting the physiological stability enhancing surface plasmon resonance (SPR) property. The targeted delivery Doxorubicin using nanopayload is demonstrated this report. drug release profile...

Cannabidiol (CBD) has been used to treat a variety of cancers and inflammatory conditions with controversial results. In previous work, we have shown that breast cancer MCF-7 cells, selected by their response IL-1β cytokine, acquire malignant phenotype (6D cells) through an epithelial–mesenchymal transition (EMT). We evaluated CBD as potential inhibitor this inducer reversion non-invasive phenotype. It decreased 6D cell viability, downregulating expression receptor CB1. The blocked migration...

Magnetic nanoparticles such as cobalt ferrite are investigated under clinical hyperthermia conditions for the treatment of cancer. Cobalt (CFNPs) synthesized by thermal decomposition method, using nonionic surfactant Triton-X100, possess hydrophilic polyethylene oxide chains acting reducing agents and iron precursors. The monodispersed were 10 nm size, confirmed high-resolution transmission electron microscopy (HR-TEM). X-ray diffraction patterns CFNPs prove existence cubic spinel ferrites....

Inflammation is a critical component of cancer development. Previously, we showed in vitro that IL-1β treatment non-invasive human breast MCF-7 cells promoted their transition to malignant phenotype (6D cells). This epithelial–mesenchymal was reverted by exposure cannabidiol (CBD). We show murine model subcutaneous inoculation 6D induced formation and development tumors, the which keep traits malignancy. These processes were interrupted administration CBD under two schemes: therapeutic...

Cancer is the second leading cause of death worldwide, despite many treatments available, cancer patients face side effects that reduce their quality life. Therefore, there a need to develop novel strategies increase efficacy treatments. In this study, gold nanoparticles obtained by green synthesis with Coffea arabica bean extract were loaded Doxorubicin, (a highly effective but non-specific drug) direct interaction and using commercial organic ligands allow colloidal dispersion at...

A novel, one-step synthesis of one-dimensional cobalt ferrite nanowhiskers (CfW) is reported.

A modified clay cup (cantarito) microbial fuel cell (C-MFCs) was designed to digest the biomass effluent from a nopal biogas (NBE). To improve process, commercial acrylic varnish (AV) applied C-MFCs. The experiment performed as:Both-C-MFCs, painting of AV on both sides cup; In-C-MFCs, internal side, and Out-C-MFCs external side. order for maximum volumetric power densities were Both-C-MFCs (1841.99 mW/m3)>Out-C-MFCs (1023.74 mW/m3) >In-C-MFCs (448.90 mW/m3). control without did not show...

Lung cancer is, currently, one of the main malignancies causing deaths worldwide. To date, early prognostic and diagnostic markers for small cell lung (SCLC) have not been systematically clearly identified, so most patients receive standard treatment. In present study, we combine quantitative proteomics studies use magnetic core-shell nanoparticles (mCSNP's), first to identify a marker cancer, second functionalize their possible application timely diagnosis this other types cancer. used...

Mesenchymal stem cells (MSCs) are self-renewing found in almost all postnatal organs and tissues the perivascular region. These present multiple characteristics that make them candidates to be applied cell therapy for neurodegenerative diseases, such as their secretory action, migration lesion area, immunomodulatory potential. have a high capacity mesodermal differentiation; however, numerous studies shown MSCs can also differentiate into neurons. However, despite positive results trials...

This study has aimed to different culture systems that might stimulate an increase in cell proliferation of normal and osteoarthritis chondrocytes from articular cartilage rat model.Three using embedded alginate beads were tested: cultured Dulbecco's modified Eagle's medium (DMEM) as control, a co-culture system consisting monolayer de-differentiated source mitotic factors, enriched containing obtained DMEM. Normal stained with 5-carboxyfluorescein diacetate succinimidyl ester each the three...

The wild type huntingtin protein (Htt), supports the production of brain-derived neurotrophic factor (BDNF), a survival for striatal neurons, through cytoplasmic sequestering RE-1silencing transcription (REST). In Huntington´s Disease an inherited degenerative disease, caused by CAG expansion in 5´coding region gene, mutant (mHtt), causes that REST enters pathologically into nucleus cells, resulting repression neuronal genes including BDNF, progressive death. It has been reported Htt...

Novel materials are explored very often by material scientists to design an efficient drug delivery system target carcinoma cells. Among various nanosystem, functionalized Iron oxide Nanoparticles (IoNP) were definitely studied especially target, endocyte and release moieties inside the This IoNP platform is usually composed of inorganic core a highly biocompatible shell layer in order perform numerous tasks at same time, such as delivery, multimodal imaging, instantaneous monitoring, along...

A point mutation from guanine (G) to adenine (A) at nucleotide position 1081 in the hemagglutinin-neuraminidase (HN) gene has been associated with neurovirulence of Urabe AM9 mumps virus vaccine. This corresponds a glutamic acid (E) lysine (K) change 335 HN glycoprotein. We have experimentally demonstrated that two variants strain (HN-A1081 and HN-G1081) differ neurotropism, sialic acidbinding affinity neuraminidase activity. In present study, we performed structure-function analysis amino...