A5011564557- Diet and metabolism studies
- Ion Transport and Channel Regulation
- Metabolism and Genetic Disorders
- Genetic Syndromes and Imprinting
- Health and Medical Education
- Muscle metabolism and nutrition
- Technology in Education and Healthcare
- Higher Education Teaching and Evaluation
- Developmental and Educational Neuropsychology
- Drug Transport and Resistance Mechanisms
- Amino Acid Enzymes and Metabolism
- Ion channel regulation and function
- RNA Research and Splicing
- Pancreatic function and diabetes
- Kruppel-like factors research
- Parkinson's Disease Mechanisms and Treatments
- Gastrointestinal motility and disorders
- Biochemical Analysis and Sensing Techniques
- Epigenetics and DNA Methylation
- Communication and COVID-19 Impact
- Animal Nutrition and Physiology
- Caveolin-1 and cellular processes
- Health, Education, and Physical Culture
- Neurogenesis and neuroplasticity mechanisms
- Problem and Project Based Learning
Universidad de Sevilla
2011-2024
University of Montana
2022
De Montfort University
2022
University of Bergen
2022
Kaunas kolegija Higher Education Institution
2022
University of Veterinary Medicine in Košice
2022
University of Malta
2022
Hospital Universitario de Fuenlabrada
2014-2015
Stony Brook University
1997-1999
State University of New York
1997
Several genes encoding proteins critical to the neuronal phenotype, such as brain type II sodium channel gene, are expressed high levels only in neurons. This cell specificity is due, part, long-term repression nonneural cells mediated by repressor protein REST/NRSF (RE1 silencing transcription factor/neural-restrictive factor). We show here that CoREST, a newly identified human protein, functions corepressor for REST. A single zinc finger motif REST required CoREST interaction. Mutations of...
Abstract Aims According to Braak's hypothesis, it is plausible that Parkinson's disease (PD) originates in the enteric nervous system (ENS) and spreads brain through vagus nerve. In this work, we studied whether inflammatory bowel diseases (IBDs) humans can progress with emergence of pathogenic α‐synuclein (α‐syn) gastrointestinal tract midbrain dopaminergic neurons. Methods We have analysed gut ventral from subjects previously diagnosed IBD form a DSS‐based rat model inflammation terms...
The type II voltage-dependent sodium channel is present in neuronal cells, where it mediates the propagation of nerve impulses. Restricted expression gene to neurons due, at least part, binding repressor protein REST (also termed NRSF or XBR) RE1 called NRSE) sequence gene. Previous studies have shown that a domain containing eight GL1-Krüppel zinc finger motifs DNA binding. Deletional and GAL4-fusion analyses now reveal domains lie outside DNA-binding both amino carboxyl termini REST....
In spite of all the fascinating properties oral creatine supplementation, mechanism(s) mediating its intestinal absorption has(have) not been investigated. The purpose this study was to characterize transport. [(14)C] uptake measured in chicken enterocytes and rat ileum, expression transporter CRT examined human, small intestine by reverse transcription-polymerase chain reaction, Northern blot, situ hybridization, immunoblotting immunohistochemistry. Results show that accumulate against...
Metabolites produced by an altered gut microbiota might mediate the effects in brain. Among metabolites, fecal volatile organic compounds (VOCs) are considered to be potential biomarkers. In this study, we examined both VOCs and bacterial taxa feces from healthy subjects Alzheimer's disease (AD) patients at early middle stages. Remarkably, 29 13 genera were differentiated AD patients. general, higher amounts of acids esters found terpenes, sulfur aldehydes subjects. At stage AD, most...
Reelin is an extracellular matrix protein that plays a critical role in neuronal migration. Here we show the mucosa of human colon expresses reelin, its receptors ApoER2 and VLDLR, effector Dab1. Immunohistochemical analyses reveal reelin expression restricted to pericryptal myofibroblasts; Dab1 detected at myofibroblasts, apical domain surface epithelial crypt cells, strong linear staining observed basement membrane; VLDLR are cytoplasm epithelium also cells. Human colorectal cancer...
L-carnitine transport has been measured in enterocytes and basolateral membrane vesicles (BLMV) isolated from chicken intestinal epithelia. In the nominally Na+-free conditions take up until cell to medium ratio is 1. This uptake was inhibited by L-carnitine, D-carnitine, gamma-butyrobetaine, acetylcarnitine, tetraethylammonium (TEA), betaine. L-3H-carnitine into BLMV showed no overshoot, it (i) Na+-independent, (ii) trans-stimulated intravesicular (iii) cis-inhibited TEA cold L-carnitine....
Abstract. The kidney efficiently salvages creatine from the urine; however, mechanism(s) that mediates renal reabsorption has not been investigated. This study characterizes transport mechanism in brush-border membrane vesicles isolated rat cortex. An osmolality plot revealed is transported into an osmotically active space and it also bound to membranes. inwardly directed NaCl gradient stimulated uptake time course of exhibited overshoot phenomenon, which indicates presence process for these...
Expression of reelin, reelin receptors [apolipoprotein E receptor 2 (ApoER2) and very low density lipoprotein (VldlR)] the Disabled-1 (Dab1) protein was investigated in rat intestinal mucosa. Intestinal Dab1 mRNA levels were maximal early stages life, reaching adult 1-month-old rats. restricted to fibroblasts, whereas mRNAs Dab1, ApoER2, VldlR integrins alpha3 beta1 observed enterocytes, crypts fibroblasts. Reelin only isolated fibroblasts a cell layer subjacent villus epithelium, which...
Intestinal myofibroblasts secrete substances that control organogenesis and wound repair of the intestine. The rat small intestine express reelin present work explores whether regulates crypt-villus unit homeostasis using normal mice with gene disrupted (reeler). results reveal mouse expresses reelin, its receptors apolipoprotein E receptor 2 (ApoER2) very low-density lipoprotein (VldlR) effector protein Disabled-1 (Dab1) expression is restricted to myofibroblasts. absence significantly...
Abstract Ulcerative colitis is a chronic pathology characterized by relapsing–remitting phases of intestinal inflammation. Additionally, some patients develop neuropsychiatric disorders, such as depression and anxiety, or cognitive deficits. We aimed to investigate whether the development elicits memory, locomotion, mood impairments. It further examined these impairments are influenced sex. Here, we used model in male female rats, induced with sodium dextran sulfate, mirroring human...
ABSTRACT We previously proposed that Dab2 participates in the endocytosis of milk macromolecules rat small intestine. Here we investigate receptors may mediate this by studying effects age and diet on megalin, VLDLR, ApoER2 expression, expression cubilin amnionless. Of VLDLR ApoER2, only megalin pattern resembles reported. Thus mRNA protein levels are high intestine suckling rat, down‐regulated up‐regulated diet, mainly ileum. Neither nor affect levels. The effect depends epithelial cell...
D-mannose transport and metabolism has been studied in enterocytes isolated from chicken small intestine. In the presence of Na+, mannose taken up by cells either remains free, is phosphorylated, catabolized to H2O, or becomes part membrane components. The remaining free cytosol released when are transferred an ice bath. Na+-dependent electrogenic inhibited ouabain dinitrophenol; its substrate specificity differs SGLT-1 transporter. Glut2 transporter inhibitors phloretin cytochalasin B added...
The effects of aldosterone and arginine vasotocin (AVT) on intestinal Na + /H exchange (NHE) -sugar cotransport (SGLT-1) activities have been investigated using brush-border membrane vesicles isolated from Hubbard chicken small large intestines, they were compared with those induced by either depletion or dehydration. was feeding the chickens a low- high-Na diet for 0.5, 1, 2, 4, 8 days. Ileal colonic NHE2 activity increased duration depletion, whereas that SGLT-1 decreased, reaching plateau...
The myofibroblasts placed underneath the epithelium of rodent small intestine express reelin, and reelin absence modifies both morphology cell renewal processes crypt-villus unit. In developing central nervous system, effects are mediated by disabled-1 (Dab1) protein. present work explores whether Dab1 mediates control unit dynamics examining in mouse consequences (i) (scrambler mutation) on (ii) (reeler intestinal expression Dab1.The scrambler mutation remarkably similar to those caused...
Parkinson’s disease is a highly prevalent neurological disorder for which there currently no cure. Therefore, the knowledge of risk factors as well development new putative molecular targets mandatory. In this sense, peripheral inflammation, especially originated in colon, emerging predisposing factor suffering disease. We have largely studied pleiotropic roles galectin-3 driving microglia-associated immune responses. However, studies aimed at elucidating role inflammation terms microglia...