A5013291364- Neuroinflammation and Neurodegeneration Mechanisms
- Genetics and Neurodevelopmental Disorders
- Neuroscience and Neuropharmacology Research
- Neurogenetic and Muscular Disorders Research
- Cellular transport and secretion
- Galectins and Cancer Biology
- Inflammation biomarkers and pathways
- Parkinson's Disease Mechanisms and Treatments
- RNA modifications and cancer
- Lipid Membrane Structure and Behavior
- Muscle Physiology and Disorders
- Epigenetics and DNA Methylation
- Lysosomal Storage Disorders Research
- RNA Research and Splicing
- Autophagy in Disease and Therapy
- Memory and Neural Mechanisms
- Immune cells in cancer
- Ubiquitin and proteasome pathways
- Olfactory and Sensory Function Studies
- Gastrointestinal motility and disorders
- Diet and metabolism studies
- Autism Spectrum Disorder Research
- RNA regulation and disease
- CRISPR and Genetic Engineering
- HIV Research and Treatment
Hospital Universitario Virgen del Rocío
2015-2025
Instituto de Biomedicina de Sevilla
2015-2025
Universidad de Sevilla
2016-2025
LETI Pharma (Germany)
2023
Universidad Católica San Antonio de Murcia
2022
Foundation for the Research Development and Application of Composite Materials
2017
Universidad Pablo de Olavide
2012-2016
Universidad de Burgos
2000
University of California, San Diego
1988-1989
Alzheimer's disease (AD) is a progressive neurodegenerative in which the formation of extracellular aggregates amyloid beta (Aβ) peptide, fibrillary tangles intraneuronal tau and microglial activation are major pathological hallmarks. One key molecules involved galectin-3 (gal3), we demonstrate here for first time role gal3 AD pathology. Gal3 was highly upregulated brains patients 5xFAD (familial disease) mice found specifically expressed microglia associated with Aβ plaques....
Molecular diversity of microglia, the resident immune cells in CNS, is reported. Whether microglial subsets characterized by expression specific proteins constitute subtypes with distinct functions has not been fully elucidated. Here we describe a subtype expressing enzyme arginase-1 (ARG1; that is, ARG1+ microglia) found predominantly basal forebrain and ventral striatum during early postnatal mouse development. microglia are enriched phagocytic inclusions exhibit molecular signature,...
Abstract Aims According to Braak's hypothesis, it is plausible that Parkinson's disease (PD) originates in the enteric nervous system (ENS) and spreads brain through vagus nerve. In this work, we studied whether inflammatory bowel diseases (IBDs) humans can progress with emergence of pathogenic α‐synuclein (α‐syn) gastrointestinal tract midbrain dopaminergic neurons. Methods We have analysed gut ventral from subjects previously diagnosed IBD form a DSS‐based rat model inflammation terms...
Proanthocyanidins are supposed to have some therapeutical properties as antioxidants and antineoplasics. Most of the proanthocyanidins, however, not commercialized since their separation from natural sources is either very expensive or well-known. In this work, feasibility application mixtures carbon dioxide alcohol under supercritical conditions for selective extraction phenolic compounds grape seeds has been studied, among them low polymerized main monomer units, (+)-catechin...
Low levels of survival motor neuron (SMN) protein result in spinal muscular atrophy (SMA), a severe genetic disease characterized by impairment and premature lethality. Although SMN is ubiquitous protein, neurons are much more vulnerable to low than other cells. To gain insight into the pathogenesis SMA, we have compared synaptic function terminals wild-type SMA mice at different ages two proximal muscles. Our results show that mutant muscle fibers fire normal action potentials...
Epigenomic mechanisms regulate distinct aspects of the inflammatory response in immune cells. Despite central role for microglia neuroinflammation and neurodegeneration, little is known about their epigenomic regulation response. Here, we show that Ten-eleven translocation 2 (TET2) methylcytosine dioxygenase expression increased upon stimulation with various inflammogens through a NF-κB-dependent pathway. We found TET2 regulates early gene transcriptional changes, leading to metabolic...
Synchronous neurotransmitter release is a highly regulated process that takes place at specializations the presynaptic membrane called active zones (AZs). The relationships between AZs, quantal release, and vesicle replenishment are not well understood in mature synapse. We have measured number, distribution, other properties of AZs mouse motor nerve terminals combined these observations with electrophysiological estimates size readily releasable pool (RRP) synaptic vesicles. On average, we...
Low levels of the Survival Motor Neuron (SMN) protein produce Spinal Muscular Atrophy (SMA), a severe monogenetic disease in infants characterized by muscle weakness and impaired synaptic transmission. We report here structural functional alterations organization organelles cytoskeleton motor nerve terminals mouse model SMA. The decrease SMN resulted clustering vesicles (SVs) Active Zones (AZs), reduction size readily releasable pool (RRP), recycling (RP) vesicles, active mitochondria...
Abstract Parkinson’s Disease (PD) is a neurodegenerative and progressive disorder characterised by intracytoplasmic inclusions called Lewy bodies (LB) degeneration of dopaminergic neurons in the substantia nigra (SN). Aggregated α-synuclein (αSYN) known to be main component LB. It has also been reported interact with several proteins organelles. Galectin-3 (GAL3) have detrimental function diseases. galactose-binding protein without catalytic activity expressed mainly activated microglial...
Proximal spinal muscular atrophy (SMA) is caused by homozygous loss or mutation of the SMN1 gene on human chromosome 5. Depending levels SMN protein produced from a second ( SMN2 ), different forms disease are distinguished. In patients with milder disease, type III IV SMA that normally reach adulthood, enlargement motor units regularly observed. However, underlying mechanisms not understood. Smn+/− mice, mouse model III/IV SMA, reveal progressive neurons and denervation endplates starting...
Alzheimer's disease (AD) is a progressive multifaceted neurodegenerative disorder for which no disease-modifying treatment exists. Neuroinflammation central to the pathology progression, with evidence suggesting that microglia-released galectin-3 (gal3) plays pivotal role by amplifying neuroinflammation in AD. However, possible involvement of gal3 disruption neuronal network oscillations typical AD remains unknown.Here, we investigated functional implications signaling on experimentally...
Adult hippocampal neurogenesis (AHN) is a process involved in numerous neurodegenerative diseases. Many researchers have described microglia as key component regulating the formation and migration of new neurons along rostral migratory stream. Caspase-3 cysteine-aspartate-protease classically considered one main effector caspases cell death program process. In addition to this classical function, we identified role protein modulator microglial function; however, its action on neurogenic...
The ubiquitin proteasome system (UPS) is implicated in protein homeostasis. One of the proteins involved this HERC1 E3 ligase, which was associated with several processes including normal development and neurotransmission at neuromuscular junction (NMJ), autophagy projection neurons, myelination peripheral nervous system, among others. tambaleante (tbl) mouse model carries spontaneous mutation Gly483Glu substitution protein. Using model, we analyzed implication ligase activity UPS,...
<sec> <title>BACKGROUND</title> Background: The accelerated aging process in Latin American countries, along with the lack of effective public health strategies to control risk factors, has led an increased prevalence non-communicable diseases (NCD) like cardiovascular and dementia, raising number older adults living a disability. Mobile (mHealth) applications offer promising opportunities encourage healthy lifestyles bridge implementation gap prevention programs. Currently, there are no...
Abstract Recently, several research groups have evaluated CAPN10 gene in polycystic ovarian syndrome (PCOS) patients and other phenotypes, including hirsutism or intermediate phenotypes of PCOS. Molecular genetic analysis indicates that different alleles may play a role PCOS susceptibility could be associated with idiopathic hirsutism. However, these observations are not exempt from controversy, because independent studies cannot replicate preliminary findings. We present haplotype-phenotype...
We monitored presynaptic exocytosis and vesicle recycling at neuromuscular junctions of transgenic mice expressing synaptopHluorin (spH), using simultaneous optical electrophysiological recordings. Synaptic transmission was indistinguishable from that in wild-type controls. Fluorescence rose during decayed monotonically after stimulus trains to the nerve, with amplitudes decay times increasing amount stimulation. The relatively large size synaptic terminals allowed us examine spatial profile...
Summary Recent data from animal models indicate that the eNOS null mice present a phenotype resemble human metabolic syndrome (hypertension, insulin resistance and hypertriglyceridemia). In this work, we have studied whether NOS3 gene, previously related to endothelial dysfunction, might role in susceptibility hypertensive patients. To carry out study, genotyped 105 patients ≤ 60 years old with two polymorphisms of gene: 1132 T>C 7164 G>T (GeneBank:AF519768.1).To check allelic...