Luís Miguel Real
A5008229565- Liver Disease Diagnosis and Treatment
- Hepatitis C virus research
- Genetic Associations and Epidemiology
- Alzheimer's disease research and treatments
- HIV Research and Treatment
- Bioinformatics and Genomic Networks
- Immune Cell Function and Interaction
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Liver Disease and Transplantation
- HIV/AIDS drug development and treatment
- Hepatitis B Virus Studies
- Immunotherapy and Immune Responses
- RNA regulation and disease
- Systemic Lupus Erythematosus Research
- Estrogen and related hormone effects
- T-cell and B-cell Immunology
- HIV-related health complications and treatments
- Hepatitis Viruses Studies and Epidemiology
- Genomic variations and chromosomal abnormalities
- Long-Term Effects of COVID-19
- Folate and B Vitamins Research
- COVID-19 Impact on Reproduction
- HIV/AIDS Research and Interventions
- Calpain Protease Function and Regulation
Hospital Universitario de Valme
2016-2025
Centro de Investigación Biomédica en Red
2022-2025
Instituto de Biomedicina de Sevilla
2013-2025
Universidad de Sevilla
2023-2025
Instituto de Salud Carlos III
2023-2025
Universidad de Málaga
2019-2024
Biomedical Research Networking Center on Neurodegenerative Diseases
2023
Fundación Seimc-Gesida
2023
Molecular Biology Consortium
2023
Fundació ACE
2021
Alzheimer's disease (AD) is a progressive neurodegenerative in which the formation of extracellular aggregates amyloid beta (Aβ) peptide, fibrillary tangles intraneuronal tau and microglial activation are major pathological hallmarks. One key molecules involved galectin-3 (gal3), we demonstrate here for first time role gal3 AD pathology. Gal3 was highly upregulated brains patients 5xFAD (familial disease) mice found specifically expressed microglia associated with Aβ plaques....
Large variability among Alzheimer's disease (AD) cases might impact genetic discoveries and complicate dissection of underlying biological pathways.Genome Research at Fundacio ACE (GR@ACE) is a genome-wide study dementia its clinical endophenotypes, defined based on AD's certainty vascular burden. We assessed the known AD loci across endophenotypes to generate categories. incorporated gene coexpression data conducted pathway analysis per category. Finally, evaluate effect heterogeneity in...
Importance An estimated 40% of dementia is potentially preventable by modifying 12 risk factors throughout the life course. However, robust evidence for most these lacking. Effective interventions should target in causal pathway to dementia. Objective To comprehensively disentangle aspects modifiable Alzheimer disease (AD) inspire new drug targeting and improved prevention. Design, Setting, Participants This genetic association study was conducted using 2-sample univariable multivariable...
BackgroundSince the beginning of coronavirus disease 2019 (COVID-19) pandemic, there has been increasing urgency to identify pathophysiological characteristics leading severe clinical course in patients infected with acute respiratory syndrome 2 (SARS-CoV-2). Human leukocyte antigen alleles (HLA) have suggested as potential genetic host factors that affect individual immune response SARS-CoV-2. We sought evaluate this hypothesis by conducting a multicenter study using HLA...
The APOE ε2 and ε4 alleles are the strongest protective risk-increasing, respectively, genetic variants for late-onset Alzheimer disease (AD). However, mechanisms linking to AD-particularly apoE protein's role in AD pathogenesis how this is affected by variants-remain poorly understood. Identifying missense addition could provide critical new insights, but given low frequency of additional variants, cohorts have previously been too small interrogate question robustly.To determine whether...
Chemokines are implicated in tumor pathogenesis, although it is unclear whether they affect human cancer progression positively or negatively. We found that activation of the chemokine receptor CCR5 regulates p53 transcriptional activity breast cells through pertussis toxin-, JAK2-, and p38 mitogen-activated protein kinase-dependent mechanisms. blockade significantly enhanced proliferation xenografts from bearing wild-type p53, but did not a mutation. In parallel, data obtained primary...
In order to identify novel loci associated with Alzheimer's disease (AD), we conducted a genome-wide association study (GWAS) in the Spanish population.We genotyped 1,128 individuals using Affymetrix Nsp I 250K chip. A sample of 327 sporadic AD patients and 801 controls unknown cognitive status from general population were included our initial study. To increase power study, combined results those four other public GWAS datasets by applying identical quality control filters same imputation...
Across multiancestry groups, we analyzed Human Leukocyte Antigen (HLA) associations in over 176,000 individuals with Parkinson's disease (PD) and Alzheimer's (AD) versus controls. We demonstrate that the two diseases share same protective association at HLA locus. HLA-specific fine-mapping showed hierarchical effects of
Loss of heterozygosity (LOH) in the short arm chromosome 6 (6p) was detected samples obtained from colon (13.8%), larynx (17.6%) and melanoma (15.3%) tumors. The parallel study HLA-antigen expression tumor tissues using locus- polymorphic-specific antibodies combination with LOH microsatellite analysis on 6p allowed us to establish that is a representative phenomenon most cells present given tissue. In cases, specific HLA alleles had been lost predominant population cells, indicating...
This study aimed to evaluate the association between follicle-stimulating hormone (FSH) efficacy and FSHR, CYP19, ESR1 ESR2 genes using single nucleotide polymorphism analyses. One hundred seventy women with conserved ovarian function undergoing controlled stimulation (COS) daily exogenous recombinant FSH administration. Women were categorized as poor responders (three or less follicles observed at end of cycle) normal (more than three follicles). The outcome is number normal/poor defined by...
To know the prevalence, incidence and factors associated with hepatitis E virus (HEV) infection in HIV-infected individuals Spain, as well to provide information on natural history of HIV/HEV coinfection.Prospective cohort study.Serum HEV IgG antibodies were tested 613 patients at baseline 2 years thereafter. Positive samples for HEV-RNA. In seroconversion, changes liver function tests, serum IgM RNA collected between final time points analyzed.One hundred sixty-one (26%) positive baseline....
Antiretroviral drugs with a lower potential to induce hepatic steatosis in human immunodeficiency virus (HIV) infection need be identified. We compared the effect of switching efavirenz (EFV) raltegravir (RAL) on among HIV-infected patients nonalcoholic fatty liver disease (NAFLD) receiving EFV plus 2 nucleoside analogues. tenofovir/emtricitabine or abacavir/lamivudine NAFLD were randomized 1:1 switch from RAL (400 mg twice daily), maintaining analogues unchanged, continue At baseline,...
Mosaic loss of chromosome Y (mLOY) is a common ageing-related somatic event and has been previously associated with Alzheimer’s disease (AD). However, mLOY estimation from genotype microarray data only reflects the degree subjects at moment DNA sampling. Therefore, phenotype associations AD can be severely age-confounded in context genome-wide association studies. Here, we applied Mendelian randomisation to construct an age-independent polygenic risk score (mloy-PRS) using 114 autosomal...
Polycystic ovary syndrome (PCOS) is characterized by chronic anovulation infertility, hyperandrogenemia, and frequently insulin resistance. This study investigated whether polymorphisms in the CAPN10 gene are related with PCOS etiology. The allelic frequencies genotypes of UCSNP-44, 43, 19, 63 were determined 55 well women polycystic ovaries 93 unrelated healthy controls using spectrofluorimetric analyses real-time PCR. Our data indicate that UCSNP-44 allele associated Spanish population (P...
Abstract Background The difficulty in elucidating the genetic basis of complex diseases roots many factors that can affect development a disease. Some these effects may interact ways, proving undetectable by current single-locus methodology. Results We have developed an analysis tool called Hypothesis Free Clinical Cloning (HFCC) to search for genome-wide epistasis case-control design. HFCC combines relatively fast computing algorithm detection, with flexibility test variety different...
Abstract Background Genetic admixture is a common caveat for genetic association analysis. Therefore, it important to characterize the structure of population under study control this kind potential bias. Results In we have sampled over 800 unrelated individuals from Spain, and genotyped them with genome-wide coverage. We carried out linkage disequilibrium, haplotype, copy-number variation (CNV) analyses, compared these estimates Spanish existing data similar efforts. Conclusions general,...
BACE1 is a key enzyme involved in the production of amyloid ß-peptide (Aß) Alzheimer's disease (AD) brains. Normally, its expression constitutively inhibited due to presence 5′untranslated region (5′UTR) promoter. activated by phosphorylation eukaryotic initiation factor (eIF)2-alpha, which reverses inhibitory effect exerted 5′UTR. There are four kinases associated with different types stress that could phosphorylate eIF2-alpha. Here we focus on double-stranded (ds) RNA-activated protein...