A5045223330- Chronic Obstructive Pulmonary Disease (COPD) Research
- COVID-19 Clinical Research Studies
- Respiratory Support and Mechanisms
- Autophagy in Disease and Therapy
- Muscle Physiology and Disorders
- Long-Term Effects of COVID-19
- Epigenetics and DNA Methylation
- Cardiovascular and exercise physiology
- Hemoglobin structure and function
- High Altitude and Hypoxia
- Inflammasome and immune disorders
- Palliative Care and End-of-Life Issues
- Mitochondrial Function and Pathology
- Heme Oxygenase-1 and Carbon Monoxide
- Erythrocyte Function and Pathophysiology
- SARS-CoV-2 and COVID-19 Research
- Metabolomics and Mass Spectrometry Studies
- Autoimmune and Inflammatory Disorders Research
- Tissue Engineering and Regenerative Medicine
- Dietary Effects on Health
- Frailty in Older Adults
- Neurogenetic and Muscular Disorders Research
- Immune responses and vaccinations
- Thermal Regulation in Medicine
- Physical Education and Training Studies
Albany Medical Center Hospital
2019-2024
Pulmonary and Critical Care Associates
2019-2023
We performed RNA-seq and high-resolution mass spectrometry on 128 blood samples from COVID-19-positive COVID-19-negative patients with diverse disease severities outcomes. Quantified transcripts, proteins, metabolites, lipids were associated clinical outcomes in a curated relational database, uniquely enabling systems analysis cross-ome correlations to molecules patient prognoses. mapped 219 molecular features high significance COVID-19 status severity, many of which involved complement...
There are no prior reports that compare differentially methylated regions of DNA in blood samples from COVID-19 patients to collected before the SARS-CoV-2 pandemic using a shared epigenotyping platform. We performed genome-wide analysis circulating CpG methylation Infinium Human MethylationEPIC BeadChip on 124 hospitalized COVID-19-positive and COVID-19-negative compared these data with previously reported 39 healthy individuals pandemic. Prospective outcome measures such as COVID-19-GRAM...
BackgroundSince the beginning of coronavirus disease 2019 (COVID-19) pandemic, there has been increasing urgency to identify pathophysiological characteristics leading severe clinical course in patients infected with acute respiratory syndrome 2 (SARS-CoV-2). Human leukocyte antigen alleles (HLA) have suggested as potential genetic host factors that affect individual immune response SARS-CoV-2. We sought evaluate this hypothesis by conducting a multicenter study using HLA...
Severe COVID-19 patients present a clinical and laboratory overlap with other hyperinflammatory conditions such as hemophagocytic lymphohistiocytosis (HLH). However, the underlying mechanisms of these remain to be explored. Here, we investigated transcriptome 1596 individuals, including in comparison healthy controls, acute inflammatory states (HLH, multisystem syndrome children [MIS-C], Kawasaki disease [KD]), different respiratory infections (seasonal coronavirus, influenza, bacterial...
We performed RNA-Seq and high-resolution mass spectrometry on 128 blood samples from COVID-19 positive negative patients with diverse disease severities. Over 17,000 transcripts, proteins, metabolites, lipids were quantified associated clinical outcomes in a curated relational database, uniquely enabling systems analysis cross-ome correlations to molecules patient prognoses. mapped 219 molecular features high significance status severity, many involved complement activation, dysregulated...
We recently reported the COVID-19-induced circulating leukocytes DNA methylation profile. Here, we hypothesized that some of these genes would persist differentially methylated after disease resolution. Fifteen participants previously hospitalized for SARS-CoV-2 infection were epityped one year discharge. Of 1505 acute illness-induced regions (DMRs) identified, found 71 with persisted methylated, an average 7 serial CpG positions per DMR. Sixty-four DMRs hypermethylated, and DMR...
The COVID19 pandemic has caused more than a million of deaths worldwide, primarily due to complications from COVID19-associated acute respiratory distress syndrome (ARDS). Controversy surrounds the circulating cytokine/chemokine profile ARDS, with some groups suggesting that it is similar patients without ARDS and others observing substantial differences. Moreover, although hyperinflammatory phenotype associates higher mortality in non-COVID19 there little information on inflammatory...
Patients with chronic obstructive pulmonary disease (COPD)-pulmonary emphysema often develop locomotor muscle dysfunction, which entails reduced mass and force-generation capacity is associated worse outcomes, including higher mortality. Myogenesis contributes to adult integrity during injury-repair cycles. Injurious events crucially occur in the skeletal muscles of patients COPD setting exacerbations infections, lead acute decompensations for limited periods time, after typically fail...
High CO2 retention, or hypercapnia, is associated with worse outcomes in patients chronic pulmonary diseases. Skeletal muscle wasting also an independent predictor of poor acute and Although previous evidence indicates that high accelerates skeletal catabolism via AMPK (AMP-activated protein kinase)-FoxO3a-MuRF1 (E3-ubiquitin ligase RING finger 1), little known about the role regulating anabolism. In present study, we investigated potential attenuating synthesis. We found locomotor muscles...
COVID-19 has been associated with Interstitial Lung Disease features. The immune transcriptomic overlap between Idiopathic Pulmonary Fibrosis (IPF) and not investigated.we analyzed blood transcript levels of 50 genes known to predict IPF mortality in three two cohorts. Scoring Algorithm Molecular Subphenotypes (SAMS) was applied distinguish high versus low-risk profiles all SAMS cutoffs derived from the Discovery cohort were used intensive care unit (ICU) status, need for mechanical...
Patients with chronic obstructive pulmonary disease (COPD) usually develop skeletal muscle dysfunction, which represents a major comorbidity in these patients and is strongly associated mortality other poor outcomes. Although clinical data indicates that accelerated protein degradation metabolic disruption are common associations of dysfunction COPD, there very limited on the mechanisms regulating process, part, due to lack research performed validated animal model emphysema. This deficiency...
Patients with pulmonary emphysema often develop locomotor muscle dysfunction, which is independently associated disability and higher mortality in that population. Muscle dysfunction entails reduced force generation capacity, partially depends on fibers' oxidative potential, yet very little mechanistic research has focused respiration emphysema. Using a recently established animal model of emphysema-driven skeletal we found downregulation SDHC (succinate dehydrogenase subunit C) association...
: Both CO2 retention, or hypercapnia, and skeletal muscle dysfunction predict higher mortality in critically ill patients. Mechanistically, injury reduced myogenesis contribute to critical illness myopathy, while hypercapnia causes wasting, no research has been conducted on hypercapnia-driven dysfunctional vivo. Autophagy flux regulates by supporting stem cell -satellite cell- activation, previous data suggests that inhibits autophagy. We tested whether worsens satellite autophagy myogenic...
Patients with acute and chronic pulmonary conditions such as obstructive disease (COPD) often develop skeletal muscle dysfunction, which is strongly independently associated poor outcomes including higher mortality. Some of these patients also CO2 retention, or hypercapnia, worse prognosis. While dysfunction in settings involve reduction mass disrupted fibers' metabolism leading to suboptimal work, mechanistic research the field has been limited by lack adequate animal models. Over last...
Over the last years, use of peripheral blood-derived big datasets in combination with machine learning technology has accelerated understanding, prediction, and management pulmonary critical care conditions. The goal this article is to provide readers an introduction methods applications blood omics other multiplex-based technologies medicine setting better appreciate current literature field. To accomplish that, we essential concepts needed rationalize approach introduce types molecules...
Identifying novel regulators of vascular smooth muscle cell function is necessary to further understand cardiovascular diseases. We previously identified cytoglobin, a hemoglobin homolog, with myogenic and cytoprotective roles in the vasculature. The specific mechanism action cytoglobin unclear but does not seem be related oxygen transport or storage like hemoglobin. Herein, transcriptomic profiling injured carotid arteries global knockout mice revealed that deletion accelerated loss...
Abstract The COVID19 pandemic is likely to cause more than a million of deaths worldwide, primarily due complications from COVID19-associated acute respiratory distress syndrome (ARDS). Controversy surrounds the circulating cytokine/chemokine profile ARDS, with some groups suggesting that it similar non-COVID19 ARDS patients and others observing substantial differences. Moreover, while hyperinflammatory phenotype associates higher mortality in there little information on inflammatory...
COVID19 pandemic has so far caused over three hundred thousand deaths worldwide, primarily due to complications from SARS-CoV-2-associated acute respiratory distress syndrome (ARDS). While an ARDS-driven hyperinflammatory phenotype is associated with higher mortality in non-COVID patients, there little information on how cytokines and chemokines expressions correlate clinical outcomes patients. We prospectively enrolled a cohort of 41 patients mechanical ventilation. Patients blood was...
Reduced skeletal muscle mass and oxidative capacity coexist in patients with pulmonary emphysema are independently associated higher mortality. If reduced cellular respiration contributes to atrophy that setting remains unknown. Using a mouse genetically induced recapitulates dysfunction, we found activity of succinate dehydrogenase (SDH) is hallmark its myopathic changes. We generated an inducible, muscle-specific SDH knockout demonstrates lower mitochondrial oxygen consumption, myofiber...
Abstract Severe COVID-19 patients present a clinical and laboratory overlap with other hyperinflammatory conditions such as hemophagocytic lymphohistiocytosis (HLH). However, the underlying mechanisms of these remain to be explored. Here, we investigated transcriptome 1596 individuals, including in comparison healthy controls, acute inflammatory states (HLH, multisystem syndrome children [MIS-C], Kawasaky disease [KD]), different respiratory infections (seasonal coronavirus, influenza,...
The oxidant hydrogen peroxide serves as a signaling molecule that alters many aspects of cardiovascular functions. Recent studies suggest cytoglobin - hemoglobin expressed in the vasculature may promote electron transfer reactions with proposed functions decomposition. Here, we determined extent to which regulates intracellular and established mechanisms. We found decreased hyperoxidation peroxiredoxins maintained activity peroxiredoxin 2 following challenge exogenous peroxide. Cytoglobin...