A5027785940- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- Monoclonal and Polyclonal Antibodies Research
- Genetic factors in colorectal cancer
- CAR-T cell therapy research
- vaccines and immunoinformatics approaches
- Reproductive System and Pregnancy
- Bladder and Urothelial Cancer Treatments
- Glycosylation and Glycoproteins Research
- Cytokine Signaling Pathways and Interactions
- Chemokine receptors and signaling
- Cervical Cancer and HPV Research
- Viral-associated cancers and disorders
- Cancer Research and Treatments
- T-cell and Retrovirus Studies
- Hemostasis and retained surgical items
- Cytomegalovirus and herpesvirus research
- interferon and immune responses
- Protein purification and stability
- Drug-Induced Adverse Reactions
- Galectins and Cancer Biology
- Cardiac Fibrosis and Remodeling
- Vitamin D Research Studies
Comunidad de Madrid
2024
Universidad de Granada
2006-2021
Hospital Universitario Virgen de las Nieves
2002-2013
Children's Hospital of Pittsburgh
2007
Universidad de Jaén
2000
Abstract: The mechanisms that lead to loss of MHC class I expression in different types tumors are not yet fully known. Accordingly, we studied colorectal carcinomas elucidate the specific evasion T‐cell immune response. We selected with total and 124 immunohistochemical staining anti‐HLA monoclonal antibodies (mAb). Fourteen (11%) exhibited a phenotype HLA loss. Microsatellite instability (MSI) analysis was also carried out same tumor samples. β 2 ‐microglobulin (β m), HLA‐A, B, C antigens,...
Abstract: Mutations have been identified in the β 2 ‐ microglobulin gene of tumor cells two metastatic melanoma patients who received immunizations with MAGE peptides. One mutation abolishes start codon whereas other introduces a premature stop codon. The second micro‐globulin allele both tumors appears to be lost on basis sequence data and loss microsatellite heterozygosity. lack product results absence HLA class I antigens surface cells. This may explain why progressed despite immunization...
Abstract: We analyzed the expression of HLA class I antigens in 78 tumor tissue samples obtained from patients diagnosed as having colorectal carcinomas. A broad panel mAbs defining monomorphic, locus‐specific and allele‐specific determinants was used. In addition, an antibody HLA‐C determinant (L31) also tested. Previous reports on these tumors indicated losses 30 to 40%. At least 73% present study had a detectable alteration. These altered phenotypes were classified total loss (18%)...
Downregulation of MHC class I expression is a widespread phenomenon used by tumor cells to escape antitumor T-cell-mediated immune responses. These alterations may play role in the clinical course disease. The aim our study was investigate molecular mechanism underlying absence HLA-class molecule bladder cancer cells. Microdissected tissues were characterized real-time quantitative PCR for HLA-ABC, beta2-microglobulin and members antigen processing machinery (APM) HLA molecules (LMP2, LMP7,...
Abstract We present the results of a comparative gene expression analysis 15 metastases (10 regressing and 5 progressing) obtained from 2 melanoma patients with mixed response following different forms immunotherapy. Whole genome transcriptional clearly indicate that regression is due to an acute immune rejection mediated by upregulation genes involved in antigen presentation interferon (STAT‐1/IRF‐1) all both patients. In contrast, progressing showed low transcription levels these pathways....
Loss of heterozygosity (LOH) in the short arm chromosome 6 (6p) was detected samples obtained from colon (13.8%), larynx (17.6%) and melanoma (15.3%) tumors. The parallel study HLA-antigen expression tumor tissues using locus- polymorphic-specific antibodies combination with LOH microsatellite analysis on 6p allowed us to establish that is a representative phenomenon most cells present given tissue. In cases, specific HLA alleles had been lost predominant population cells, indicating...
Human papillomavirus types HPV 16 and 11 DNA sequences were analyzed in normal neoplastic tissues of the larynx, using technique polymerase chain reaction (PCR). An amplified region E6 ORF was hybridized with 3' end-labelled oligonucleotide probe. Twenty six out 48 (54%) squamous-cell carcinomas, 3 verrucous-cell carcinomas sequences, whereas we did not detect sequences. also found normal, autologous mucosa lymphnode metastases, although these absent other analyzed. HPV-16-positive tumors...