A5006022642- Immune Cell Function and Interaction
- HIV Research and Treatment
- COVID-19 Clinical Research Studies
- T-cell and B-cell Immunology
- Long-Term Effects of COVID-19
- SARS-CoV-2 and COVID-19 Research
- Immune cells in cancer
- Immunotherapy and Immune Responses
- IL-33, ST2, and ILC Pathways
- Tryptophan and brain disorders
- Mast cells and histamine
- Urticaria and Related Conditions
- Food Allergy and Anaphylaxis Research
- CAR-T cell therapy research
- HIV/AIDS drug development and treatment
- Cytomegalovirus and herpesvirus research
- Phagocytosis and Immune Regulation
- Immune Response and Inflammation
- Cancer, Hypoxia, and Metabolism
- Reproductive System and Pregnancy
- Viral Infections and Immunology Research
- Eosinophilic Esophagitis
- Sepsis Diagnosis and Treatment
- Asthma and respiratory diseases
- Effects of Radiation Exposure
Hospital Universitario Virgen del Rocío
2021-2025
Instituto de Biomedicina de Sevilla
2021-2025
Universidad de Sevilla
2021-2025
BioCruces Health research Institute
2015-2023
University of the Basque Country
2019
Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV)-2 infection induces an exacerbated inflammation driven by innate immunity components. Dendritic cells (DCs) play a key role in the defense against viral infections, for instance plasmacytoid DCs (pDCs), have capacity to produce vast amounts of interferon-alpha (IFN-α). In COVID-19 there is deficit DC numbers and IFN-α production, which has been associated with disease severity. this work, we described that addition deficiency, several...
Abstract Natural Killer (NK) cells acquire memory-like properties following a brief stimulation with IL-12, IL-15 and IL-18. These IL-12/15/18-preactivated NK cells, also known as cytokine-induced (CIML) have been revealed powerful tool in cancer immunotherapy due to their persistence the host increased effector functions. Several studies shown that modulate metabolism response cytokine-stimulation other stimuli, suggesting there is link between cellular In this paper, we analyzed metabolic...
Background: Persistent controllers (PC) maintain antiretroviral-free HIV-1 control indefinitely over time while transient (TC) eventually lose virological control. It is essential to characterize the quality of HIV reservoir these phenotypes identify factors that lead progression and open new avenues in cure strategies. Methods: The characterization reservoir, from peripheral blood mononuclear cells, was performed using next-generation sequencing techniques, such as full-length individual...
A brief in vitro stimulation of natural killer (NK) cells with interleukin (IL)-12, IL-15 and IL-18 endow them a memory-like behavior, characterized by higher effector responses when they are restimulated after resting period time. These preactivated NK cells, also known as cytokine-induced (CIML) have several properties that make promising tool cancer immunotherapy. In the present study, we described effect different combinations IL-12, on generation human CIML cells. Our data points to...
The ubiquitin proteasome system (UPS) is implicated in protein homeostasis. One of the proteins involved this HERC1 E3 ligase, which was associated with several processes including normal development and neurotransmission at neuromuscular junction (NMJ), autophagy projection neurons, myelination peripheral nervous system, among others. tambaleante (tbl) mouse model carries spontaneous mutation Gly483Glu substitution protein. Using model, we analyzed implication ligase activity UPS,...
Background Despite the efficacy of SARS-CoV-2 vaccines in reducing mortality and severe cases COVID-19, a proportion survivors experience long-term symptoms, known as post-acute sequelae infection (PASC). This study investigates immunological neurodegenerative effects associated with extracellular vesicles (EVs) COVID-19 survivors, 15 months after infection. Methods 13 Controls 20 infection, were recruited. Pro-inflammatory cytokines analyzed both plasma EVs. A deep-immunophenotyping...
Neonates are more susceptible to infections than adults. This susceptibility is thought reflect neonates' qualitative and quantitative defects in the adaptive innate immune responses. Differential expression of cell surface receptors may result altered thresholds neonatal activation. We determined whether function lipid-binding CD300 family different on cells compared adult cells. A multiparametric flow cytometry analysis was performed determine peripheral blood mononuclear cord The CD300a...
The immune factors associated with impaired SARS-CoV-2 vaccine response in elderly people are mostly unknown. We studied individuals older than 60 and younger years, who had been vaccinated BNT162b2 mRNA, before after the first second dose. Aging was a lower anti–RBD IgG levels decreased magnitude polyfunctionality of SARS-CoV-2–specific T cell response. dramatic decrease thymic function > which fueled alteration homeostasis, their CD161+ were 2 months vaccination. Additionally, deficient DC...
Plasmacytoid dendritic cells (pDCs) sense viral and bacterial products through Toll-like receptor (TLR)-7 -9 translate this sensing into Interferon-α (IFN-α) production T-cell activation. The understanding of the mechanisms involved in pDCs stimulation may contribute to HIV-cure immunotherapeutic strategies. objective present study was characterize immunomodulatory effects TLR agonist stimulations several HIV-1 disease progression phenotypes non infected donors.pDCs, CD4 CD8 T-cells were...
Intra-host evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been reported in cases with persistent disease 2019 (COVID-19). In this study, we describe a severely immunosuppressed individual HIV-1/SARS-CoV-2 coinfection long-term course SARS-CoV-2 infection. A 28-year-old man was diagnosed HIV-1 infection (CD4+ count: 3 cells/µL nd 563000 RNA copies/mL) and simultaneous
Natural killer (NK) cells are usually identified by the absence of other lineage markers, due to lack cell-surface-specific receptors. CD56neg NK cells, classically as CD56negCD16+, very scarce in peripheral blood healthy people but they expand some pathological conditions. However, studies on had revealed different results regarding phenotype and functionality. This could be to, among others, unstable expression CD16, which hinders cells' proper identification. Hence, we aim determine an...
Abstract SARS‐CoV‐2 specific T‐cell response has been associated with disease severity, immune memory and heterologous to endemic coronaviruses. However, an integrative approach combining a comprehensive analysis of the quality antibody levels in these three scenarios is needed. In present study, we found that, acute infection, while mild was high polyfunctionality biased IL‐2 production inversely correlated anti‐S IgG levels, combinations only including IFN‐γ absence perforin predominated...
Basophils express high-affinity IgE receptors (FcεRI), which play an essential role in allergic diseases. It is important to characterize new cell-surface that modulate IgE-mediated basophil activation threshold design promising immunomodulatory therapies.We sought analyze the expression of CD300 on human basophils and their implication processes.Blood samples from healthy subjects patients with cow's milk allergy were collected through Basque Biobank under institutional review...
SUMMARY Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV)-2 infection induces an exacerbated inflammation driven by innate immunity components. Dendritic cells (DCs) play a key role in the defense against viral infections, for instance plasmacytoid DCs (pDCs), have capacity to produce vast amounts of interferon-alpha (IFN-α). In COVID-19 there is deficit DC numbers and IFN-α production, which has been associated with disease severity. this work, we described that addition deficiency,...
We evaluated the safety and viral rebound, after analytical treatment interruption (ATI), of vedolizumab ART in recent HIV-1 infection. used this model to analyze impact α4β7 on reservoir size. Participants started with monthly Vedolizumab infusions ATI was performed at week 24. Biopsies were obtained from ileum caecum baseline levels, reservoir, flow cytometry cell-sorting antibody competition experiments assayed. safe well-tolerated. No participant achieved undetectable viremia off 24...
The ability of the CD300a inhibitory receptor to modulate immune cell functions and its involvement in pathogenesis many diseases has aroused a great interest this molecule. Within human CD4+ T lymphocytes from healthy donors, is differentially expressed among different helper subsets. However, there are no data about expression regulation on cells HIV-1 infected patients. objective study was investigate HIV-infected patients suppressive combined antiretroviral treatment (cART) cART naïve...