A5020291130- Hepatitis Viruses Studies and Epidemiology
- Viral gastroenteritis research and epidemiology
- Liver Disease and Transplantation
- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- Hepatitis B Virus Studies
- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Vector-Borne Animal Diseases
- Liver Diseases and Immunity
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Veterinary medicine and infectious diseases
- Immune Cell Function and Interaction
- HIV-related health complications and treatments
- Animal Disease Management and Epidemiology
- HIV/AIDS Research and Interventions
- Cytomegalovirus and herpesvirus research
- T-cell and Retrovirus Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Viral Infections and Immunology Research
- Parasitic Infections and Diagnostics
- Artificial Intelligence in Healthcare
- Imbalanced Data Classification Techniques
- COVID-19 Clinical Research Studies
Instituto Maimónides de Investigación Biomédica de Córdoba
2016-2025
Hospital Universitario Reina Sofía
2016-2025
University of Córdoba
2016-2025
Instituto de Salud Carlos III
2022-2025
Centro de Investigación Biomédica en Red
2022-2025
Friedrich-Loeffler-Institut
2024
Instituto de Investigación de Enfermedades Raras
2017
National University of the Northeast
2007-2009
•First cases of acute hepatitis related to Orthohepevirus C infection in Europe.•Second registered death worldwide an immunosuppressed individual.•Screening for RNA should be evaluated all patients with hepatitis. Background & AimHepatitis E virus (HEV) was considered the only member Hepeviridae family zoonotic potential. Nevertheless, this consideration has been reassessed owing several reported and chronic linked genus. Because circulation rodents described worldwide, risk transmission is...
Background: Persistent controllers (PC) maintain antiretroviral-free HIV-1 control indefinitely over time while transient (TC) eventually lose virological control. It is essential to characterize the quality of HIV reservoir these phenotypes identify factors that lead progression and open new avenues in cure strategies. Methods: The characterization reservoir, from peripheral blood mononuclear cells, was performed using next-generation sequencing techniques, such as full-length individual...
Summary An HIV‐infected patient was diagnosed with acute hepatitis E infection in our hospital. epidemiological inquiry performed to collect demographic, food and animal exposure variables order identify the potential route of transmission. The reported that his family traditionally hunted wild boar for food. All members were analysed virus infection. Additionally, transmission by meat consumption prevalence HEV among from same hunting area investigated. In all‐family ( n = 8), HEV‐RNA...
We conducted a cross-sectional study in wild boar and extensively managed Iberian pig populations hotspot area of Crimean-Congo hemorrhagic fever virus (CCHFV) Spain. tested for antibodies against CCHFV by using 2 ELISAs parallel. assessed the presence RNA means reverse transcription quantitative PCR protocol, which detects all genotypes. A total 113 (21.8%) 518 suids sampled showed ELISA. By species, 106 (39.7%) 267 boars 7 (2.8%) 251 pigs analyzed were seropositive. Of 231 analyzed, none...
The hepatitis E virus (HEV) represents a major cause of acute worldwide. majority HEV cases occur in low-income countries, mainly Asia and Africa, where causes large outbreaks associated with the consumption contaminated water high mortality specific populations. In high-income infection is considered zoonotic disease that linked to food. Although proportion have self-limiting asymptomatic or subclinical infections, immunosuppression may modify pathogenesis clinical impact this emerging...
Abstract Rat hepatitis E virus (ratHEV-C1) is a newly zoonotic and globally distributed of increasing public health concern. Although rats the genus Rattus are main reservoir this emerging virus, role other small mammals in epidemiology ratHEV-C1 Europe unknown as large-scale survey studies specifically assessing circulation these species have not yet been conducted. In study, we examined active infection 514 animals from seven different mammal sampled throughout Spain. The presence viral...
ABSTRACT Introduction Liver steatosis (LS) is a condition that characterised by hepatic fat accumulation unrelated to significant alcohol consumption. This study explored the serum proteomic profile associated with LS in people living HIV (PLWH). Methods The cohort comprised 266 PLWH, 21.1% and 78.9% of whom had no LS, respectively. Serum samples were analysed using liquid chromatography coupled mass spectrometry (LC–MS). Results Among 220 proteins detected, afamin (AFM) apolipoprotein F...
It has been shown that wildlife can serve as natural reservoirs of hepatitis E virus (HEV). The wild boar (Sus scrofa) is probably the main reservoir HEV and could therefore represent an important route transmission in Europe, especially regions where game meat widely consumed. We evaluated prevalence infection south Spain, with aim identifying associated risk factors. A cross-sectional study included hunted was carried out during 2015/2016 hunting season (October 15 to February 15)...
The objective of this study was to design a pangenotypic PCR-based assay for the detection and quantification hepatitis E virus (HEV) RNA from across entire spectrum described genotypes belonging Orthohepevirus A genus. optimal conditions performance were determined by testing WHO standard strain (6219/10) HEV panel (8578/13). Similarly, comparisons made with two commercial assays (Real Star RT-PCR 2.0 ampliCube Quant) detect at concentrations below 1,000 IU/ml viral strains test samples 54...
Abstract To evaluate the diagnostic value of combination two broad‐range PCR assays targeting different and conserved regions viral genome for diagnosis acute Hepatitis E virus (HEV) infection. Patients with hepatitis were prospectively recruited. In all, HEV‐IgM antibodies tested together evaluation HEV viraemia by (ORF3 ORF1). The number individuals exhibiting negative IgM antibody results but carrying RNA was calculated each assay. Four‐hundred seventy included, whom 145 (30.8%) diagnosed...
Hepatitis E (HE) is an important emerging disease in European countries. To analyse the role of equids as potential reservoirs for HE virus (HEV), we determined prevalence HEV infection 861 equines from 464 herds Spain. RNA serum was detected 0.4% (3/692) horses, 1.2% (1/86) donkeys and 3.6% (3/83) mules. Phylogenetic analysis identified zoonotic genotype 3 being closely related to viral human swine strains. In this first report on Europe, confirm susceptibility mules infection. The low...
Hepatitis E virus (HEV) is an emerging zoonotic pathogen with pigs and wild boar serving as reservoirs for human infection through direct contact infected animals or the consumption of raw undercooked pork products. The liver considered main target site HEV replication in swine important organ pathogenesis disease. aim this study was to characterize cells entry naturally evaluate type severity pathological changes order reach a better understanding hepatic pathogenic mechanisms involved...
Wild boar (Sus scrofa) is considered as the main wildlife reservoir of zoonotic hepatitis E virus (HEV) genotypes. The aim this study was to evaluate circulation HEV in free-ranging wild Doñana National Park (DNP), Spain. Blood samples were collected from 99 DNP during 2015. Sera analysed parallel using indirect ELISA and real-time RT-PCR. A total 57 tested animals (57.6%; 95%CI: 47.8%-67.3%) had anti-HEV antibodies, indicating that widespread DNP. RNA detected one animal phylogenetic...
Abstract Several studies have reported the persistence of HCV RNA in liver and/or peripheral blood mononuclear cells (PBMCs) spite undetectable viremia patients who achieved sustained virological response (SVR). This event, defined as occult infection, remains controversial and low titers persistent virus may be underestimated because it has not yet been analyzed by a highly sensitive test such droplet digital PCR (ddPCR). method provides an alternate ultra-sensitive detection technique for...
Hepatitis E virus (HEV) represents a major health problem worldwide. As the course of HEV cases is often subclinical, asymptomatic infections could represent an important source viral spread and infection via routes such as blood donations. Before universal screening for in donations can be implemented, studies evaluating incidence are needed to establish potential risk transmission. This prospective longitudinal study that included donors recruited at Hospital de Ciudad Real Transfusion...
Numerous protist species are shared between humans and pigs. Among those, Giardia duodenalis, Cryptosporidium spp. Balantioides coli have a clear public animal health significance. For others such as Enterocytozoon bieneusi Blastocystis sp., their impact on has not been fully established. Little information is currently available the molecular diversity of these protists in swine populations. To fill this gap, we molecularly assessed G. spp., B. coli, sp. E. faecal samples from Iberian Large...
Our study aim was to describe and characterize the global Hepatitis E virus (HEV) molecular genotype geographical distribution in domestic pig wild boar, which could facilitate traceability of human cases. We performed a systematic sequence search for HEVs identified boar from available data GenBank. Only sequences with lengths greater than 300 nt were included. For all sequences, length, host (i.e., or boar), country origin, HEV genotype/subtype recorded. Genotypes assigned by HEVnet typing...
ABSTRACT Background Nonalcoholic fatty liver disease (NAFLD) is an increasing concern for people living with HIV (PLWH). However, information on the impact of NAFLD prognosis PLWH very scarce. Aims To investigate influence overall and liver‐related mortality in PLWH. Methods followed three Spanish centres were included a prospective cohort at date first transient elastography evaluation. Survival data recorded, causes death centrally monitored. The risk all‐cause was evaluated by applying...
Fatty liver disease (FLD) is frequently observed in HIV-infected patients and a cause of advanced disease. Genetic factors could play role determining risk for FLD development those patients. The aim this study was to evaluate the association single nucleotide polymorphisms (SNPs) previously found be related nonalcoholic by genome-wide analyses general population with presence FLD, including steatohepatitis, individuals.This transversal study.A total 431 were included study. All them...
Contradictory data about the impact of rs738409 steatosis-related polymorphism within PNPLA3 gene on liver fibrosis progression in HIV/hepatitis C virus (HIV/HCV)-coinfected patients have been reported. Our objective was to test whether this, and other polymorphisms previously related fatty disease HIV infection linked SAMM50 or LPPR4 genes, influence HIV/HCV-coinfected individuals. Three hundred thirty two who consecutively attended four Spanish university hospitals from November 2011 July...