A5034905489- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Vector-Borne Animal Diseases
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- T-cell and B-cell Immunology
- Virology and Viral Diseases
- Insect symbiosis and bacterial influences
- Malaria Research and Control
- Microbial infections and disease research
- Bat Biology and Ecology Studies
- Biochemical Analysis and Sensing Techniques
- Biosensors and Analytical Detection
- Genomics and Phylogenetic Studies
- Regulation of Appetite and Obesity
- Biochemical and Molecular Research
- Genetic and phenotypic traits in livestock
- Vector-borne infectious diseases
- Fire effects on ecosystems
- COVID-19 Clinical Research Studies
- Mycobacterium research and diagnosis
- Plant Virus Research Studies
- Neurological diseases and metabolism
- COVID-19 epidemiological studies
- Zoonotic diseases and public health
Friedrich-Loeffler-Institut
2021-2025
University of Castilla-La Mancha
2024
Consejo Superior de Investigaciones Científicas
2024
University of Córdoba
2024
Ferdowsi University of Mashhad
2009
West Nile virus (WNV) and Usutu (USUV) are important flaviviruses circulating in Germany. While USUV was first reported more than 10 years ago, WNV has only reached the country 2018. Wild birds amplifying hosts for both viruses. Therefore, we have been monitoring bird population different regions of Germany by a previously established network many years. This report summarizes results molecular and/or serological methods 2345 blood samples from 22 orders over 2900 carcasses 2019 2020. RNA...
We conducted a cross-sectional study in wild boar and extensively managed Iberian pig populations hotspot area of Crimean-Congo hemorrhagic fever virus (CCHFV) Spain. tested for antibodies against CCHFV by using 2 ELISAs parallel. assessed the presence RNA means reverse transcription quantitative PCR protocol, which detects all genotypes. A total 113 (21.8%) 518 suids sampled showed ELISA. By species, 106 (39.7%) 267 boars 7 (2.8%) 251 pigs analyzed were seropositive. Of 231 analyzed, none...
The nucleoside analog N4-hydroxycytidine (NHC) is the active metabolite of prodrug molnupiravir, which has been approved for treatment COVID-19. SARS-CoV-2 incorporates NHC into its RNA, resulting in defective virus genomes. Likewise, inhibitors dihydroorotate dehydrogenase (DHODH) reduce yield upon infection, by suppressing cellular synthesis pyrimidines. Here, we show that and DHODH strongly synergize inhibition replication vitro. We propose lack available pyrimidine nucleotides increases...
Abstract Summary Virus surveillance programmes are designed to counter the growing threat of viral outbreaks human health. Nanopore sequencing, in particular, has proven be suitable for this purpose, as it is readily available and provides rapid results. However, special bioinformatic required extract relevant information from sequencing data, applications needed that allow users without extensive bioinformatics knowledge carry out analysis steps. We present VirDetector, a pipeline virus...
Abstract Background Bats and rodents have been identified as reservoirs for several highly pathogenic zoonotic viruses including henipaviruses, a genus within the Paramyxoviridae family. A number of studies revealed circulation henipaviruses at wildlife-human-livestock interface in Cameroon. In this study, we describe molecular analysis well development evaluation Bead-based Multiplex Binding Assay (BMBA) using an in-house Indirect Enzyme Linked Immunosorbent (ELISA) to confirm detection...
Abstract West Nile virus (WNV) is a mosquito-borne flavivirus mainly circulating in eastern Germany, causing annually reoccurring epizootics the avifauna as well sporadic infections humans and horses. WNV closely-related to Usutu (USUV) tick-borne encephalitis (TBEV) co-infections thereof are becoming more frequent. To not solely be dependent on monitoring of wild birds horses availability alternative sentinel species advantageous. The study examined seroprevalence antibodies (Abs) Germany...
West Nile virus (WNV) is known to cause disease and death in humans various animals worldwide. WNV has circulated Germany since 2018. In 2020, four birds tested positive for the genome at Zoopark Erfurt (Thuringia). Moreover, neutralization assays detected neutralizing antibodies (nAb) against 28 birds. addition, nAb Usutu (USUV) were found 14 To protect valuable reduce risk of viral transmission from humans, we performed a field study on vaccination zoo. conduct study, 61 zoo categorized...
In Mauritania, several mosquito-borne viruses have been reported that can cause devastating diseases in animals and humans. However, monitoring data on their occurrence local distribution are limited. Rift Valley fever virus (RVFV) is an arthropod-borne causes major outbreaks throughout the African continent Arabian Peninsula. The first (RVF) epidemic Mauritania occurred 1987 since then country has affected by recurrent of disease. To gain information RVFV as well other vectors we collected...
Golden Syrian hamsters (Mesocricetus auratus) are used as a research model for severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2). Millions of also kept pets in close contact to humans. To determine the minimum infective dose (MID) assessing zoonotic transmission risk, and define optimal infection experimental studies, we orotracheally inoculated with SARS-CoV-2 doses from 1 * 10
Abstract The structure of cellular prion proteins encoded by the protein gene ( PRNP ) impacts susceptibility to transmissible spongiform encephalopathies, including chronic wasting disease (CWD) in deer. recent emergence CWD Northern European reindeer Rangifer tarandus ), moose Alces alces and red deer Cervus elaphus parallel with outbreak North America, gives reason investigate variation deer, implement risk assessments adjust management for populations under threat. We here report...
Dugbe orthonairovirus (DUGV) and Crimean-Congo hemorrhagic fever (CCHFV) are tick-borne arboviruses within the order Bunyavirales. Both viruses endemic in several African countries can induce mild (DUGV, BSL 3) or fatal (CCHFV, 4) disease humans. Ruminants play a major role their natural transmission cycle. Therefore, they considered as suitable indicator animals for serological monitoring studies to assess risk human infections. Although both do not actually belong same serogroup,...
Usutu virus (USUV) and West Nile (WNV) are known to cause diseases mortalities in bird populations. Since 2010/2011, USUV has circulated Germany spread nationwide, while WNV was only introduced into East 2018. The zoological garden investigated is located Northern Germany, where infections wild birds have been detected for several years. In this longitudinal study conducted over a four-year period, zoo were sampled biannually screened molecular serological evidence of WNV. genomes eight the...
Usutu virus (USUV) is a mosquito-borne flavivirus that widely distributed in southern and central Europe. The zoonotic circulates primarily between birds mosquitoes, can, however, rare cases infect other mammals including humans. In the past, USUV has been repeatedly associated with mass mortalities birds, blackbirds owls. Birds commonly succumb either due to peracute nature of infection or severe encephalitis. Germany, spread rapidly since its first detection 2010 mosquitoes under presence...
Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne zoonotic orthonairovirus of public health concern and widespread geographic distribution. Several animal species are known to seroconvert after infection with CCHFV without showing clinical symptoms. The commercial availability multi-species ELISA has led an increase in recent serosurveillance studies as well the range reported be exposed field, including wild boar (Sus scrofa). However, development validation confirmatory...
Abstract Background Usutu virus (USUV) and West Nile (WNV) are zoonotic arthropod-borne orthoflaviviruses. The enzootic transmission cycles of both include Culex mosquitoes as vectors birds amplifying hosts. For more than ten years, these viruses have been monitored in Germany by a multidisciplinary network. While USUV is present nationwide, WNV used to be restricted the central-east. Methods In 2021 2022, over 2300 live bird blood samples organs from 3000 deceased were subjected molecular...
Usutu virus (USUV) and West Nile (WNV) are zoonotic arthropod-borne orthoflaviviruses. The enzootic transmission cycles of both include
Usutu virus (USUV) is a mosquito-borne zoonotic and one of the causes flavivirus encephalitis in birds occasionally humans. USUV rapidly disperses susceptible host vector environment, as case South Central Europe. However, compared to other flaviviruses, has received less research attention there therefore limited access whole-genome sequences also in-depth phylogenetic phylodynamic analyses. To ease future molecular studies, this study compares first- (partial sequencing via Sanger),...
On the African continent, a large number of arthropod-borne viruses (arboviruses) with zoonotic potential have been described, and yet little is known most these pathogens, including their actual distribution or genetic diversity. In this study, we evaluated as proof-of-concept effectiveness nonspecific sequencing technique sequence-independent single primer amplification (SISPA) on third-generation techniques (MinION sequencing, Oxford Nanopore Technologies, Oxford, UK) by comparing results...
This study describes genetic variability in the leptin Iranian native, Brown Swiss and Holstein cattle (Bos Indicus Bos Taurus). is first of polymorphism gene native cattle. We examined exon 2 from 587 individuals six different populations cattles (86 Sarabi, 66 Taleshi, 94 Sistani, 76 Golpayegani, 104 161 cattle) using PCR-RFLP method. Analysis frequencies various alleles each breed indicated that allele C with 68, 55, 69, 71, 55 57% value were most frequent alleles. Observed...
The bovine lymphocyte antigen (BoLA-DRB3) gene encodes cell surface glycoproteins that initiate immune responses by presenting processed antigenic peptides to CD4 T helper cells. DRB3 is the most polymorphic MHC class II which peptide-binding groove. Since different alleles favor binding of peptides, has been extensively evaluated as a candidate marker for associations with various diseases and immunological traits. Therefore, in this study, genetic diversity locus two Iranian subpopulations...
The aim of this study was to identify the deficiency uridine monophosphate synthase (DUMPS) and complex vertebral malformation (CVM) in Iranian Holstein bulls. A total 144 blood samples were prepared bulls Abbas Abad Animal Breeding Center Ferdowsi University Mashhad's Dairy Farm Khorasan state Iran. Genomic PCR-RFLP protocol performed amplify polymorphic region bovine UMPS gene. Also, genomic PCR-SSCP method for CVM solute carrier family 35 member 3 (SLC35A3) genes. results...
Abstract Usutu virus (USUV) is a mosquito-borne flavivirus that widely distributed in southern and central Europe. The zoonotic circulates primarily between birds mosquitoes, can, however, rare cases infect other mammals including humans. In the past USUV has been associated with mass mortalities birds, formerly blackbirds owls. Birds commonly succumb either due to peracute nature of infection or severe encephalitis. Germany, spread rapidly since its first detection 2010 mosquitoes under...
Since the identification of Hendra virus (HeV) infections in horses Australia 1994, more than 80 outbreaks have been reported, and four out seven spillover humans had a fatal outcome. With availability subunit vaccine based on HeV-Glycoprotein (HeV-G), there is need to serologically Differentiate Infected from Vaccinated Animals (DIVA). We developed an indirect ELISA using HeV-G expressed Leishmania tarentolae HeV-Nucleoprotein (HeV-N) recombinant baculovirus-infected insect cells as...