A5076308485- Influenza Virus Research Studies
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Animal Disease Management and Epidemiology
- Herpesvirus Infections and Treatments
- Virology and Viral Diseases
- Virus-based gene therapy research
- HIV Research and Treatment
- Viral Infections and Vectors
- Respiratory viral infections research
- Mosquito-borne diseases and control
- Microbial infections and disease research
- Viral Infections and Immunology Research
- Poxvirus research and outbreaks
- Immunotherapy and Immune Responses
- Rabies epidemiology and control
- Immune Cell Function and Interaction
- Malaria Research and Control
- T-cell and B-cell Immunology
- SARS-CoV-2 and COVID-19 Research
- Vector-Borne Animal Diseases
- T-cell and Retrovirus Studies
- Bacteriophages and microbial interactions
- COVID-19 epidemiological studies
- Immune Response and Inflammation
Leipzig University
2014-2024
Institute of Virology of the Slovak Academy of Sciences
2002-2018
CS Diagnostics
2018
Bangladesh Agricultural University
2017
Infectious Diseases Institute
2016
Friedrich-Loeffler-Institut
2005-2012
North Carolina State University
2002-2007
Utrecht University
1995-1999
Rega Institute for Medical Research
1997
KU Leuven
1997
Influenza virus A/H1N1, which is currently causing a pandemic, contains gene segments with ancestors in the North American and Eurasian swine lineages. To get insights into replication dynamics, clinical symptoms transmission pigs, we infected animals intranasally influenza A/Regensburg/D6/09/H1N1. Virus excretion inoculated pigs was detected nasal swabs from 1 day post-infection (p.i.) onwards developed generally mild symptoms, including fever, sneezing, discharge diarrhoea. Contact became...
Wild birds play an important role as reservoir hosts and vectors for zoonotic arboviruses foster their spread. Usutu virus (USUV) has been circulating endemically in Germany since 2011, while West Nile (WNV) was first diagnosed several bird species horses 2018. In 2017 2018, we screened 1709 live wild zoo with real-time polymerase chain reaction serological assays. Moreover, organ samples from carcasses submitted were investigated. Overall, 57 blood of the (2017 2018), 100 dead (2017)...
West Nile virus (WNV) and Usutu (USUV) are important flaviviruses circulating in Germany. While USUV was first reported more than 10 years ago, WNV has only reached the country 2018. Wild birds amplifying hosts for both viruses. Therefore, we have been monitoring bird population different regions of Germany by a previously established network many years. This report summarizes results molecular and/or serological methods 2345 blood samples from 22 orders over 2900 carcasses 2019 2020. RNA...
A natural reassortant influenza virus consisting of seven genome segments from pandemic (H1N1) 2009 and a neuraminidase segment Eurasian porcine H1N1 was detected in pig herd Germany. The obvious reassortment compatibility between the viruses origin raises concern as to whether swine may become reservoir for further reassortants with unknown implications human health production.
Abstract Disease progression of feline immunodeficiency virus (FIV) infection is characterized by up-regulation B7.1 and B7.2 costimulatory molecules their ligand CTLA4 on CD4+ CD8+ T cells. The CD4+CTLA4+B7+ phenotype described in FIV+ cats reminiscent CD4+CD25+CTLA4+ cells, a for immunosuppressive regulatory (Treg) In the present study, we describe phenotypic functional characteristics CD4+CD25+ cells PBMC lymph nodes (LN) control cats. Similar to Treg but not CD4+CD25− directly isolated...
Abstract Experiments that exposed influenza virus (H5N1)–infected cats to susceptible dogs did not result in intraspecies or interspecies transmission. Infected showed increased body temperatures, viral RNA pharyngeal swabs, and seroconversion but fatal disease.
Highly pathogenic avian influenza viruses (HPAIV) differ from all other strains by a polybasic cleavage site in their hemagglutinin. All these HPAIV share the H5 or H7 subtype. In order to investigate whether acquisition of an avirulent virus strain with hemagglutinin than is sufficient for immediate transformation into HPAIV, we adapted A/Duck/Ukraine/1/1963 (H3N8) that A/Chicken/Italy/8/98 (H5N2), A/Chicken/HongKong/220/97 (H5N1), A/Chicken/Germany/R28/03 (H7N7) and generated recombinant...
In early 2006, the highly pathogenic avian influenza virus (HPAIV) H5N1 of Asian lineage caused death wild aquatic birds in Northern Germany. mainly affected areas, a trans-species transmission HPAIV to mammals occurred between and domestic cats 1 Stone Marten ( Martes foina), respectively. Here, we report lesions distribution antigen 3 infected naturally with A/swan/Germany/R65/06. The hemagglutinin partial nucleotide sequences viruses were genetically closely related obtained from dead...
ABSTRACT From infection studies with cultured chicken cells and experimental mammalian hosts, it is well known that influenza viruses use the nonstructural protein 1 (NS1) to suppress synthesis of interferon (IFN). However, our current knowledge regarding in vivo role virus-encoded NS1 chickens much more limited. Here, we report highly pathogenic avian subtypes H5N1 H7N7 lacking fully functional genes were attenuated 5-week-old chickens. Surprisingly, diseased birds infected mutants, IFN...
Usutu virus (USUV) is a mosquito-borne flavivirus accounting for large-scale deaths in resident bird populations. In this study, we show the introduction of USUV to Eastern Germany resulting massive death birds, particularly blackbirds (Turdus merula). We found that three diverse lineages ("Europe 3," "Africa 2," and 3-like") circulated simultaneously. Moreover, detected Culex pipiens region where no dead birds were reported, strengthening need mosquito monitoring uncover spread arboviruses....
Influenza A viruses are maintained as a quasispecies cloud in several natural host reservoirs of avian well mammalian species. Accidental exposure, selection and further adaptation individual influenza during sporadic trans-species transmission may eventually lead to the establishment new, stably circulating lineages possibly mammalian, Given high transmissibility such virus susceptible, immunologically naive population, pandemic spread within short time ensue. In April 2009, novel...
Paramyxoviruses comprise a large number of diverse viruses which in part give rise to severe diseases affected hosts. A new genotype feline morbillivirus, tentatively named morbillivirus 2 (FeMV-GT2), was isolated from urine cats with urinary tract diseases. Whole genome sequencing showed about 78% nucleotide homology known morbilliviruses. The virus permanent cell lines and simian origin. To investigate the tropism FeMV-GT2 primary epithelial cells kidney, bladder lung, peripheral blood...
Species A rotaviruses (RVAs) are a major cause of gastroenteritis in animals and humans. Their genome consists 11 segments dsRNA, reassortment events between animal human strains can contribute to the high genetic diversity RVAs. We used plasmid-based reverse genetics system investigate potential segment encoding viral outer capsid protein VP4, which is antigenic determinant, mediates entry plays an important role host cell tropism. rescued reassortant viruses containing VP4 from porcine,...
West Nile virus (WNV) infections were first detected in Germany 2018, but information about WNV seroprevalence horses is limited. The study’s overall goal was to gather that would help veterinarians, horse owners, and veterinary-, public health- authorities understand the spread of direct protective measures. For this purpose, determined counties with without previously registered horses, risk factors for seropositivity estimated. cohort consisted privately owned from nine Eastern Germany. A...
The B7.1 and B7.2 costimulatory molecules on antigen-presenting cells provide second signals for regulating T cell immune responses via CD28 cytotoxic lymphocyte antigen 4 (CTLA4) cells. proliferation, whereas CTLA4 anergy or apoptosis, terminating the response. Because apoptosis immunodeficiency is a characteristic of feline virus (FIV)-infected cats, it possible that negative signaling B7 may be favored in these cats. Flow cytometry revealed high percentages CD8+ CD4+ expressing B7.1,...
2',3'-Didehydro-2',3'-dideoxythymidine (d4T) and its lipophilic 5'-monophosphate triester prodrug, So324, were evaluated for their antiretroviral metabolic properties in four different animal species cell lines. The antiretrovirus activity of So324 was approximately 4-10-fold greater than that d4T against human immunodeficiency virus types 1 2 simian T lymphocyte CEM MT-4 cells feline Crandell kidney cells, 50-fold visna sheep choroid plexus but 5-fold inferior murine (Moloney) sarcoma...