A5087861209- Parasitic Infections and Diagnostics
- Amoebic Infections and Treatments
- Parasites and Host Interactions
- Parasitic infections in humans and animals
- Viral gastroenteritis research and epidemiology
- Toxoplasma gondii Research Studies
- Enterobacteriaceae and Cronobacter Research
- Child Nutrition and Water Access
- Parasite Biology and Host Interactions
- Helminth infection and control
- Wildlife Ecology and Conservation
- Study of Mite Species
- Coccidia and coccidiosis research
- Hepatitis Viruses Studies and Epidemiology
- Congenital Anomalies and Fetal Surgery
- Antibiotic Use and Resistance
- Clostridium difficile and Clostridium perfringens research
- Vector-borne infectious diseases
- Plant and Fungal Interactions Research
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Dermatological diseases and infestations
- Animal Disease Management and Epidemiology
- Antibiotic Resistance in Bacteria
- Zoonotic diseases and public health
Centro Nacional de Microbiologia
2019-2024
Instituto de Salud Carlos III
2019-2024
University of Aveiro
2024
Universidad Complutense de Madrid
2024
National Centre for Social research
2024
Three recent studies of Blastocystis epidemiology in mammalian hosts identified four novel sequences that appeared to share B. lapemi as the most similar sequence. However, full-length ssu rRNA gene were not available confirm validity these new subtypes. In present study, Nanopore MinION sequencing was used obtain reference for each Additionally, phylogenetic analyses and pairwise distance comparisons performed We propose described this study should be assigned subtype designations...
Abstract The Blastocystis subtype ST10 has been recognized to contain a great deal of diversity at the sequence level, potentially indicating presence multiple new STs within clade. However, data needed validate these were not available. To help resolve this diversity, full‐length small subunit ( SSU ) rRNA gene reference sequences generated using Oxford Nanopore MinION long‐read sequencing from 21 samples representing domestic and wild hosts geographic regions covering previously described...
We assessed the occurrence, genetic diversity, and zoonotic potential of four protozoan (Cryptosporidium spp., Entamoeba histolytica, dispar, Giardia duodenalis), one stramenopile (Blastocystis sp.), microsporidia (Enterocytozoon bieneusi), two ciliate (Balantioides coli, Troglodytella abrassarti) intestinal parasite or commensal protist species in captive non-human primates (NHP) their zookeepers from six European zoological gardens France (n = 1), Germany Spain 4). Faecal samples NHP 454)...
Enteropathogenic parasites and viruses have been frequently reported in swine can infect a wide range of mammals, including humans. Among the variety infecting swine, diarrhoeagenic protists are among those that cause significant morbidity. Hepatitis E virus (HEV) has also both domestic pigs wild boar is known to an important public health significance. These agents share fecal−oral transmission route, but data on their fecal shedding circulation pathways still lacking or incomplete. Hence,...
Little information is currently available on the epidemiology of parasitic and commensal protist species in captive non-human primates (NHP) their zoonotic potential. This study investigates occurrence, molecular diversity, potential transmission dynamics a zoological garden southern Spain. The prevalence genotypes main enteric were investigated faecal samples from NHP (n = 51), zookeepers 19) free-living rats 64) by (PCR sequencing) methods between 2018 2019. presence Leishmania spp. was...
Background Intestinal helminths, including Soil-Transmitted Helminth (STH), and Gastrointestinal Protist (GP) infections are major contributors to the global burden of disease, particularly in low-income countries such Ecuador. Their epidemiology these settings is largely unknown. Methodology This prospective cross-sectional study investigates carriage intestinal STH, GP asymptomatic schoolchildren (3–11 years) Chimborazo Guayas provinces, Single stool samples ( n = 372) epidemiological...
The ongoing increase in wild boar populations across Europe has fostered human-wildlife conflicts, including the transmission of emerging pathogens with zoonotic importance. Blastocystis is a ubiquitous, faecal-oral transmitted protist that can cause gastrointestinal illnesses and observed humans animals worldwide. role wildlife epidemiology insufficiently understood. Thus, we investigated occurrence subtype diversity free-ranging boars from Iberian Peninsula using conventional PCR...
Enteric parasites including Giardia duodenalis, Cryptosporidium spp., and to a lesser extent, Blastocystis sp. Enterocytozoon bieneusi, are major worldwide contributors diarrhoeal disease. Assessing their molecular frequency diversity is important ascertain the sources of infection, transmission dynamics, zoonotic potential. Little information available on genotypes these pathogens circulating in apparently healthy children. Here, we show that asymptomatic carriage G. duodenalis (17.4%, 95%...
Some enteric parasites causing zoonotic diseases in livestock have been poorly studied or even neglected. This is the case stramenopile Blastocystis sp. and microsporidia Enterocytozoon bieneusi Spain. transversal molecular epidemiological survey aims to estimate prevalence diversity of E. cattle faecal samples (n = 336) province Álava, Northern Initial detection was carried out by polymerase chain reaction (PCR) Sanger sequencing small subunit (ssu) rRNA gene internal transcribed spacer...
The phylum Microsporidia encompasses a diverse group of obligate, intracellular, and spore-forming organisms able to infect wide range animal hosts. Among them, Enterocytozoon bieneusi is the most frequently reported species in humans animals. Little known about presence epidemiology E. wildlife. We investigated occurrence genetic diversity wild domestic mammals, through molecular-detection methods, from different regions across Portugal. A total 756 samples were collected 288, 242, 226...
Pet dogs and cats exert an unquestionable beneficial effect in the well-being of their owners, but can also act as a source zoonotic infections if improperly cared.We investigated occurrence, risk factors, genetic variability potential intestinal parasites attended clinical veterinary setting Spain.Canine (n = 252) feline 35) faecal samples were collected during 2017-2019 analysed by coproparasitological methods. A rapid lateral immunochromatographic test (ICT) was used for detecting Giardia...
Background By mid-September 2023, several event notifications related to cryptosporidiosis had been identified from different regions in Spain. Therefore, a request for urgent notification of cases the National Surveillance Network was launched. Aim We aimed at assessing extent increase cases, epidemiological characteristics and transmission modes compared previous years. Methods analysed data on case notifications, outbreak reports genotypes focusing June–October 2023 results 2016–2022....
Abstract Background Several studies have independently evaluated the occurrence of hepatitis E virus (HEV) and enteroparasites in swine, but no surveys been conducted to jointly assess prevalence genetic diversity pigs wild boars, their sympatric transmission between hosts, potential interaction with HEV. Methods We prospectively collected serum faecal samples from black Iberian domestic boars southern Spain 2015‒2016. for HEV faeces, presence ( Giardia duodenalis , Cryptosporidium spp.,...
Micromammals have historically been recognized as highly contentious species in terms of the maintenance and transmission zoonotic pathogens to humans. Limited information is currently available on epidemiology potential public health significance intestinal eukaryotes wild micromammals. We examined 490 faecal samples, grouped into 155 pools, obtained from 11 micromammal captured Spanish provinces for presence DNA Cryptosporidium spp., Giardia duodenalis, Enterocytozoon bieneusi Blastocystis...
Domestic dogs and cats can be a source of human infection by wide diversity zoonotic pathogens including parasites. Genotyping subtyping tools are useful in assessing the true public health relevance canine feline infections these pathogens. This study investigated occurrence, genetic diversity, potential common diarrhea-causing enteric protist parasites household Egypt, country where this information is particularly scarce.In prospective, cross-sectional total 352 individual fecal samples...
Enterocytozoon bieneusi is a human pathogen with broad range of animal hosts. Initially, E. was considered an emerging opportunistic in immunocompromised, mainly HIV-infected patients, but it has been increasingly reported apparently healthy individuals globally. As other African countries, the molecular epidemiology Mozambique remains completely unknown. Therefore, we undertook study to investigate occurrence and genetic diversity infections children gastrointestinal symptoms as well...
Microsporidia is a phylum of obligate emergent intracellular protist-like fungi pathogens that infect broad range hosts including vertebrates and invertebrates. Enterocytozoon bieneusi the most common cause microsporidiosis in humans, affecting primarily immunosuppressed patients but also reported immunocompetent individuals. Epidemiological information on presence molecular diversity E. livestock wildlife Spain limited. Therefore, occurrence this microsporidia was investigated sympatric...
Infections by the protist enteroparasites Giardia duodenalis, Cryptosporidium spp., and, to a much lesser extent, Blastocystis sp. are common causes of childhood diarrhoea in low-income countries. This molecular epidemiological study assesses frequency and diversity these pathogens faecal samples from asymptomatic schoolchildren (n = 807) symptomatic children seeking medical attention 286) Zambézia province, Mozambique. Detection characterisation was conducted polymerase chain reaction...
Few studies have investigated the occurrence of microeukaryotic gut parasites in dromedary camels Egypt, and majority these investigations are based on microscopic analysis fecal material.Herein, we assessed occurrence, molecular diversity, zoonotic potential protozoan (Cryptosporidium spp. Giardia duodenalis) microsporidian (Enterocytozoon bieneusi) pathogens individual samples (n = 102) with 26) without 76) diarrhea from Aswan Governorate, Upper Egypt. Other factors possibly associated an...