A5026431948- Nerve injury and regeneration
- Parkinson's Disease Mechanisms and Treatments
- Neuroscience of respiration and sleep
- Neurogenesis and neuroplasticity mechanisms
- Ion channel regulation and function
- Ion Transport and Channel Regulation
- Neurological disorders and treatments
- Neuroscience and Neuropharmacology Research
- Cerebrospinal fluid and hydrocephalus
- Physiological and biochemical adaptations
- ATP Synthase and ATPases Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Long-Term Effects of COVID-19
- Cancer, Hypoxia, and Metabolism
- Signaling Pathways in Disease
- RNA Research and Splicing
- High Altitude and Hypoxia
- Traumatic Brain Injury and Neurovascular Disturbances
- Multiple Sclerosis Research Studies
- Systemic Lupus Erythematosus Research
- RNA regulation and disease
- Peripheral Neuropathies and Disorders
- SARS-CoV-2 and COVID-19 Research
- Heat shock proteins research
Instituto de Biomedicina de Sevilla
2014-2023
Hospital Universitario Virgen del Rocío
2014-2023
Universidad de Sevilla
2014-2023
Biomedical Research Networking Center on Neurodegenerative Diseases
2014-2023
Clinical Research Management
2020
Consejo Superior de Investigaciones Científicas
2015-2018
Instituto de Salud Carlos III
2014-2017
Centro de Investigación Biomédica en Red
2011-2014
Hospital Universitario Virgen de las Nieves
2003
Stony Brook University
1995-2002
Expression of the type II voltage-dependent sodium channel gene is restricted to neurons by a silencer element active in nonneuronal cells. We have cloned cDNA coding for transcription factor (REST) that binds this element. recombinant REST protein confers ability silence reporter genes neuronal cell types lacking native protein, whereas expression dominant negative form cells relieves silencing mediated protein. transcripts developing mouse embryos are detected ubiquitously outside nervous...
Membrane excitability in different tissues is due, large part, to the selective expression of distinct genes encoding voltage-dependent sodium channel. Although predominant channels brain, skeletal muscle, and cardiac muscle have been identified, major channel types responsible for within peripheral nervous system remained elusive. We now describe deduced primary structure a channel, nerve type 1 (PN1), which expressed at high levels throughout targeted terminals cultured dorsal root...
Acentral tenet of nerve growth factor (NGF) action that is poorly understood its ability to mediate cytoplasmic signaling, through receptor TrkA, initiated at the terminal and conveyed soma. We identified an NGF-induced protein we termed Pincher (pinocytic chaperone) mediates endocytosis trafficking NGF TrkA. In PC12 cells, overexpression dramatically stimulated unexpectedly sites clathrin-independent macropinocytosis within cell surface ruffles. Subsequently, a system Pincher-containing...
The type II voltage-dependent sodium channel is present in neuronal cells, where it mediates the propagation of nerve impulses. Restricted expression gene to neurons due, at least part, binding repressor protein REST (also termed NRSF or XBR) RE1 called NRSE) sequence gene. Previous studies have shown that a domain containing eight GL1-Krüppel zinc finger motifs DNA binding. Deletional and GAL4-fusion analyses now reveal domains lie outside DNA-binding both amino carboxyl termini REST....
O(2) is essential for aerobic life, and the classic view that it diffuses freely across plasma membrane. However, measurements of permeability lipid bilayers have indicated much lower than previously thought, therefore, existence membrane channels has been suggested. We hypothesized that, besides its role as a water channel, aquaporin-1 (AQP-1) could also work an transporter, because this transmembrane protein appears to be CO(2)-permeable highly expressed in cells with rapid turnover...
Carotid body (CB) glomus cells are highly dopaminergic and express the glial cell line derived neurotrophic factor. The intrastriatal grafting of CB aggregates exerts actions on nigrostriatal neurons in animal models Parkinson disease (PD).We conducted a phase I-II clinical study to assess feasibility, long term safety, neurochemical effects autotransplantation patients with PD.Thirteen advanced PD underwent bilateral stereotactic implantation into striatum. They were assessed before surgery...
Abstract Despite the different animal models of Parkinson's disease developed during last years, they still present limitations modelling slow and progressive process neurodegeneration. Here, we undertook a histological, neurochemical behavioural analysis new chronic parkinsonian mouse model generated by subcutaneous administration low doses MPTP (20 mg/kg, 3 times per week) for months, using both young adult aged mice. The ‐induced nigrostriatal neurodegeneration was accompanied decrease in...
Aquaporin 1 (AQP1), expressed in epithelial cells of the choroid plexus, and aquaporin 4 (AQP4) present ependymal glia limitants have been proposed to play a significant role cerebrospinal fluid (CSF) production homeostasis. However, specific contribution each water channel these functions remains unknown, being subject debate during last years. Here, we analyzed detail how AQP1 AQP4 participate different aspects CSF homeostasis such as load drainage ventricles, further explored if proteins...
AQP3 has been correlated with higher transport of glycerol, increment ATP content, and larger proliferation capacity. Recently, we described the gold(III) complex Auphen as a very selective potent inhibitor AQP3's glycerol permeability (Pgly). Here evaluated effect on various mammalian cell lines differing in expression level: no (PC12), moderate (NIH/3T3) or high (A431) endogenous expression, cells stably expressing (PC12-AQP3), human HEK293T transiently transfected (HEK-AQP3) for...
The autonomic nervous system is a master regulator of homeostatic processes and stress responses. Sympathetic noradrenergic nerve fibers decrease bone mass, but the role cholinergic signaling in has remained largely unknown. Here, we describe that early postnatally, subset sympathetic undergoes an interleukin-6 (IL-6)-induced switch upon contacting bone. A neurotrophic dependency mediated through GDNF-family receptor-α2 (GFRα2) its ligand, neurturin (NRTN), established between bone-embedded...
Aquaporin-1 (AQP1) is a water channel that highly expressed in tissues with rapid O2 transport. It has been reported this protein contributes to gas permeation (CO2, NO and O2) through the plasma membrane. We show hypoxia increases Aqp1 mRNA levels tissues, namely mouse brain lung, cultured cells, 9L glioma cell line. Stopped-flow light-scattering experiments confirmed an increase permeability of cells exposed hypoxia, supporting view hypoxic up-regulation functional role. To investigate...
Key points The carotid body (CB) is a key chemoreceptor organ that mediates the hyperventilatory response to hypoxia, and contributes process of acclimatisation chronic hypoxaemia. Knowledge CB physiology at cellular molecular levels has advanced considerably in recent times thanks studies on lower mammals; however, information humans practically absent. Here we describe properties human cells slice preparations or after enzymatic dispersion. Besides glomus (type I) glia‐like, sustentacular...
OBJECTIVE In this study, we assessed the feasibility of autotransplantation carotid body (CB) cell aggregates into striatum for treatment patients with Parkinson's disease (PD). METHODS Six advanced PD underwent bilateral CB striatum. They were evaluated clinically preoperatively and 18 months after surgery according to recommendations Core Assessment Program Intracerebral Transplantation. RESULTS No major complications or adverse events resulted from implantation surgical procedures. During...
The functional properties of most sodium channels are too similar to permit identification specific channel types underlying macroscopic current. Such discrimination would be particularly advantageous in the nervous system which different family isoforms coexpressed same cell. To test whether members mu-conotoxin can discriminate among known neuronal types, we examined six toxins for their ability block heterologously expressed channels. PIIIA blocked rat brain type II/IIA (rBII/IIA) and...
We studied the mechanisms underlying long-term functional recovery of hemiparkinsonian rats grafted intrastriatally with carotid body (CB) cell aggregates. Amelioration their motor syndrome is a result trophic actions these grafts on remaining ipsilateral substantia nigra neurons rather than release dopamine from CB grafts. The maintain stable morphological appearance and differentiated phenotype for duration life host. Adult expresses high levels glial line-derived neurotrophic factor...
Oxidative damage to dopaminergic nigrostriatal (DNS) neurons plays a central role in the pathogenesis of Parkinson's disease (PD). Glucose-6-phosphate dehydrogenase (G6PD) is key cytoprotective enzyme that provides NADPH, major source reducing equivalents cell. Mutations this are most common enzymopathies worldwide. We have studied vivo G6PD overexpressed specifically DNS pathway and show increase activity soma axon terminals neurons, separately from other or glial cells, protects them...
Aquaporin‐1 (AQP1) has been associated with tumor development. Here, we investigated how AQP1 may affect cell proliferation. The proliferative rate of adult carotid body (CB) cells, known to proliferate under chronic hypoxia, was analyzed in wild‐type (AQP1 +/+ ) and knock out −/− mice, maintained normoxia or exposed hypoxia while BrdU administered. Fewer numbers total + TH‐BrdU cells were observed indicating a role for CB Then, by flow cytometry, cycle state proliferation overexpressing...
Glial cell line-derived neurotrophic factor (GDNF) exerts a notable protective effect on dopaminergic neurons in rodent and primate models of Parkinson's disease (PD). The clinical applicability this therapy is, however, hampered by the need durable stable GDNF source allowing safe continuous delivery trophic into brain parenchyma. Intrastriatal carotid body (CB) autografting is neuroprotective potentially useful PD. It induces long-term recovery parkinsonian animals through nigrostriatal...