A5102022098- Ion Transport and Channel Regulation
- Ion channel regulation and function
- Fatty Acid Research and Health
- Meat and Animal Product Quality
- Plant Stress Responses and Tolerance
- Erythrocyte Function and Pathophysiology
- Lipid metabolism and biosynthesis
- Endoplasmic Reticulum Stress and Disease
- Adipose Tissue and Metabolism
- Muscle metabolism and nutrition
- Animal Nutrition and Physiology
- ATP Synthase and ATPases Research
- Chemical Synthesis and Reactions
- Pluripotent Stem Cells Research
- RNA regulation and disease
- Physiological and biochemical adaptations
- Pesticide Residue Analysis and Safety
- Muscle and Compartmental Disorders
- Zeolite Catalysis and Synthesis
- Education and Digital Technologies
- Redox biology and oxidative stress
- Fermentation and Sensory Analysis
- Neurological diseases and metabolism
- Parkinson's Disease Mechanisms and Treatments
- Central Venous Catheters and Hemodialysis
Federal Center for Technological Education of Minas Gerais
2024
Hospital Universitário da Universidade de São Paulo
2023
Hospital of St. Francis Xavier
2022
A. N. Nesmeyanov Institute of Organoelement Compounds
2019
Institute of General and Inorganic Chemistry
2019
Advisory Board Company (United States)
2019
University of Lisbon
2013-2018
Instituto Politécnico de Lisboa
2016
Instituto de Investigação Científica Tropical
2013
Rede de Química e Tecnologia
2009-2012
Aquaporins (AQPs) are membrane channels that conduct water and small solutes such as glycerol involved in many physiological functions. Aquaporin-based modulator drugs predicted to be of broad potential utility the treatment several diseases. Until today few AQP inhibitors have been described suitable candidates for clinical development. Here we report on potent inhibition AQP3 by gold(III) complexes screened human red blood cells (hRBC) AQP3-transfected PC12 a stopped-flow method. Among...
Abstract Aquaporins (AQPs) are membrane water/glycerol channels with essential roles in biological systems, as well being promising targets for therapy and imaging. Using a stopped‐flow method, series of gold(III), platinum(II) copper(II) complexes bearing nitrogen donor ligands, such 1,10‐phenatroline, 2,2′‐bipyridine, 4,4′‐dimethyl‐2,2′‐bipyridine, 4,4′‐diamino‐2,2′‐bipyridine 2,2′;6′,2“‐terpyridine, were evaluated human red blood cells expressing AQP1 AQP3, responsible water glycerol...
Aquaporin-5 (AQP5) is a membrane water channel widely distributed in human tissues that was found up-regulated different tumors and considered implicated carcinogenesis organs systems. Despite its wide distribution pattern physiological importance, AQP5 short-term regulation not reported mechanisms underlying involvement cancer are well defined. In this work, we expressed rat yeast investigated of gating, as AQP5's ability to facilitate H₂O₂ plasma diffusion. We can be gated by extracellular...
Aquaporin-1 (AQP1) is a water channel that highly expressed in tissues with rapid O2 transport. It has been reported this protein contributes to gas permeation (CO2, NO and O2) through the plasma membrane. We show hypoxia increases Aqp1 mRNA levels tissues, namely mouse brain lung, cultured cells, 9L glioma cell line. Stopped-flow light-scattering experiments confirmed an increase permeability of cells exposed hypoxia, supporting view hypoxic up-regulation functional role. To investigate...
A MIP (Major Intrinsic Protein) subfamily called Uncharacterized Proteins (XIP) was recently described in several fungi and eudicot plants. In this work, we cloned a XIP from grapevine, VvXIP1, agrobacterium-mediated transformation studies Nicotiana benthamiana revealed that the encoded aquaporin shows preferential localization at endoplasmic reticulum membrane. Stopped-flow spectrometry vesicles aqy-null yeast strain YSH1172 overexpressing VvXIP1 showed is unable to transport water but...
Water diffusion through biological membranes is facilitated by aquaporins, members of the widespread major intrinsic proteins (MIPs). In present study, localization, expression, and functional characterization a small basic protein (SIP) from grapevine were assessed. VvSIP1 was expressed in leaves berries field-grown vines, stems vitro plantlets, but not roots. When tobacco mesophyll cells Saccharomyces cerevisiae, fluorescent-tagged localized at endoplasmic reticulum (ER). Stopped-flow...
Natural nitrogen isotopic signature reveals deprotonation during ammonium transport across living organisms.
The pH gating of human AQP3 and its effects on both water glycerol permeabilities have been fully characterized for the first time using a red blood cell model (hRBC).
The aquaglyceroporin AQP7, a family member of aquaporin membrane channels, facilitates the permeation water and glycerol through cell membranes is crucial for body lipid energy homeostasis. Regulation permeability via AQP7 considered promising therapeutic strategy towards fat-related metabolic complications. Here, we used yeast aqy-null strain heterologous expression functional analysis human investigated its regulation by pH. Using combination in vitro silico approaches, found that changes...
Aquaporins are membrane channels that facilitate the transport of water and other small molecules across cellular membranes. We examined role six aquaporins Vitis vinifera (cv. Touriga nacional) in atypical substrates (other than water) an aqy-null strain Saccharomyces cerevisiae. Their functional characterization for was performed by stopped-flow fluorescence spectroscopy. The evaluation permeability coefficients (Pf) activation energies (Ea) revealed three (VvTnPIP2;1, VvTnTIP1;1...
Several studies revealed consistent overlap between synucleinopathies and tauopathies, demonstrating that α-synuclein (ASYN) tau co-localize in neurofibrillary tangles Lewy bodies from Alzheimer's Parkinson's disease patients corresponding animal models. Additionally, it has been shown ASYN can act as an initiator of aggregation phosphorylation these two proteins directly interact. Despite evidences, the cellular pathway implicated this synergistic interaction remains to be clarified. The...
In the meat industry, manipulation of fat deposition in cattle is pivotal importance to improve production efficiency, carcass composition and ultimately quality. There an increasing interest identification key factors molecular mechanisms responsible for development specific depots. This study aimed at elucidating influence breed diet on adipose tissue membrane permeability fluidity their interplay bovines. Two Portuguese autochthonous breeds, Alentejana Barrosã, recognized as late-...
The purpose of this study was to evaluate the effect fish oil upon egg quality, production, plasma lipids and yolk cholesterol levels laying hens. 89-week-old hens were fed diets containing 0.5%, 1%, 2%, 3% 4% for 5 weeks. It observed that addition 1% diet reduced weight, but did not affect feed conversion. Albumen shell quality impaired by supplementation diet. No significant changes on triglyceride levels, content affected oil.
The production of pork with moderate amounts intramuscular fat (IMF) without an increase in subcutaneous is highly desirable for the meat industry. Several studies indicate that dietary protein reduction during growing-finishing period pigs enhances IMF content, but its consequence on carcass deposition still contradictory. In this study, we hypothesized effects reduced diets (RPD), corrected or not limiting amino acid lysine, from distinct genotypes are mediated by adipose membranes...