A5016915042- Ion Transport and Channel Regulation
- ATP Synthase and ATPases Research
- Adipose Tissue and Metabolism
- Plant Stress Responses and Tolerance
- Pancreatic function and diabetes
- Metabolism and Genetic Disorders
- Lipid metabolism and biosynthesis
- Electrolyte and hormonal disorders
- Plant nutrient uptake and metabolism
- Diet, Metabolism, and Disease
- Fatty Acid Research and Health
- Membrane-based Ion Separation Techniques
- Metal complexes synthesis and properties
- Fungal and yeast genetics research
- Infectious Encephalopathies and Encephalitis
- Diet and metabolism studies
- Magnesium in Health and Disease
- Hormonal Regulation and Hypertension
- Erythrocyte Function and Pathophysiology
- Birth, Development, and Health
- Ion channel regulation and function
- Lipid Membrane Structure and Behavior
- Renal and related cancers
- Pharmacological Effects and Toxicity Studies
- Nanoparticle-Based Drug Delivery
University of Lisbon
2016-2025
Institute for Medical Research
2022
The Graduate Center, CUNY
2019
City University of New York
2019
Instituto Politécnico de Lisboa
2016-2017
University of Groningen
2013-2014
Rede de Química e Tecnologia
2006-2013
Universidade Nova de Lisboa
1997-2013
Aquaporins (AQPs) are membrane channels that conduct water and small solutes such as glycerol involved in many physiological functions. Aquaporin-based modulator drugs predicted to be of broad potential utility the treatment several diseases. Until today few AQP inhibitors have been described suitable candidates for clinical development. Here we report on potent inhibition AQP3 by gold(III) complexes screened human red blood cells (hRBC) AQP3-transfected PC12 a stopped-flow method. Among...
ABSTRACT Objective For a long time Aquaporin‐7 has been the only aquaporin associated with adipose tissue, and its dysregulation linked to underlying mechanisms of obesity. However, presence alternative glycerol channels within tissue postulated, which prompted us search alternate transport routes in adipocytes. In view this, it is hypothesized that Aquaporin‐11 (AQP11) would have role adipocyte cell biology. Methods The expression, localization function human AQP11 (hAQP11) cultured...
Obesity is a major threat to global health and metabolically associated with glycerol homeostasis. Here we demonstrate that in human adipocytes, the decreased pH observed during lipolysis (fat burning) correlates increased release stimulation of aquaglyceroporin AQP10. The crystal structure AQP10 determined at 2.3 Å resolution unveils molecular basis for modulation-an exceptionally wide selectivity (ar/R) filter unique cytoplasmic gate. Structural functional (in vitro vivo) analyses disclose...
Reactive oxygen species (ROS), including H2O2, contribute to oxidative stress and may cause cancer initiation progression. However, at low concentrations, H2O2 can regulate signaling pathways modulating cell growth, differentiation, migration. A few mammalian aquaporins (AQPs) facilitate diffusion across membranes participate in tumorigenesis. AQP3 AQP5 are strongly expressed tissues AQP3-mediated transport has been related breast migration, but studies with human lacking. Here, we report...
Abstract Aquaporins (AQPs) are membrane water/glycerol channels with essential roles in biological systems, as well being promising targets for therapy and imaging. Using a stopped‐flow method, series of gold(III), platinum(II) copper(II) complexes bearing nitrogen donor ligands, such 1,10‐phenatroline, 2,2′‐bipyridine, 4,4′‐dimethyl‐2,2′‐bipyridine, 4,4′‐diamino‐2,2′‐bipyridine 2,2′;6′,2“‐terpyridine, were evaluated human red blood cells expressing AQP1 AQP3, responsible water glycerol...
AQP3 has been correlated with higher transport of glycerol, increment ATP content, and larger proliferation capacity. Recently, we described the gold(III) complex Auphen as a very selective potent inhibitor AQP3's glycerol permeability (Pgly). Here evaluated effect on various mammalian cell lines differing in expression level: no (PC12), moderate (NIH/3T3) or high (A431) endogenous expression, cells stably expressing (PC12-AQP3), human HEK293T transiently transfected (HEK-AQP3) for...
Aquaporin-5 (AQP5) is a membrane water channel widely distributed in human tissues that was found up-regulated different tumors and considered implicated carcinogenesis organs systems. Despite its wide distribution pattern physiological importance, AQP5 short-term regulation not reported mechanisms underlying involvement cancer are well defined. In this work, we expressed rat yeast investigated of gating, as AQP5's ability to facilitate H₂O₂ plasma diffusion. We can be gated by extracellular...
Abstract Glycerol is an important metabolite for the control of lipid accumulation in white adipose tissue (WAT) and liver. We aimed to investigate whether exogenous administration leptin improves features non-alcoholic fatty liver disease (NAFLD) leptin-deficient ob/ob mice via regulation AQP3 AQP7 (glycerol channels mediating glycerol efflux adipocytes) AQP9 (aquaglyceroporin facilitating influx hepatocytes). Twelve-week-old male wild type were divided three groups as follows: control,...
Abstract Aquaporin‐3 (AQP3) is a membrane channel with dual aquaglyceroporin/peroxiporin activity, facilitating the diffusion of water, glycerol and H 2 O across cell membranes. AQP3 shows aberrant expression in melanoma its role adhesion, migration proliferation well described. Gold compounds were shown to modulate activity reduced associated toxicity, making them promising molecules for cancer therapy. In this study, we validated phenotype resulting from AQP3‐silencing two lines, MNT‐1...
Grapevine (Vitis vinifera L.) is one of the oldest and most important perennial crops being considered as a fruit ligneous tree model system in which water status appears crucial for high wine quality, controlling productivity alcohol level. V. genome contains 28 genes coding aquaporins, acting concerted regulated manner appear relevant plant withstanding extremely unfavorable drought conditions essential quality berries wine. Several Vv aquaporins have been reported to be expressed roots,...
Aquaporin-1 (AQP1) is a water channel that highly expressed in tissues with rapid O2 transport. It has been reported this protein contributes to gas permeation (CO2, NO and O2) through the plasma membrane. We show hypoxia increases Aqp1 mRNA levels tissues, namely mouse brain lung, cultured cells, 9L glioma cell line. Stopped-flow light-scattering experiments confirmed an increase permeability of cells exposed hypoxia, supporting view hypoxic up-regulation functional role. To investigate...
Aquaporin-5 (AQP5) and -3 (AQP3) are protein channels that showed to be up-regulated in a variety of tumors. Our goal was investigate the expression pattern AQP5 AQP3 pancreatic ductal adenocarcinomas (PDA) correlate with cell proliferation, tumor stage progression, clinical significance.35 PDA samples different stages differentiation locations were analyzed by immunohistochemistry for AQP5, several markers proliferation tumorigenesis.In overexpressed apical membrane intercalated...
Development of liposomal formulations Cuphen, a potent copper-based aquaporin inhibitor with therapeutic potential against melanoma and colon cancer.Cuphen was incorporated into liposomes using the dehydration-rehydration method. The ability Cuphen to induce cancer cell death evaluated by MTS ViaCount assays. In vivo toxicity studies were performed in BALB/c mice.In vitro illustrated antiproliferative effects different lines, free form or after incorporation liposomes. revealed no toxic...
The mechanism of inhibition water and glycerol permeation <italic>via</italic> human aquaglyceroporin-3 (AQP3) by gold(<sc>iii</sc>) complexes has been described, for the first time, using molecular dynamics (MD), combined with density functional theory (DFT) electrochemical studies.