A5035294991- SARS-CoV-2 and COVID-19 Research
- Congenital heart defects research
- COVID-19 Clinical Research Studies
- Cardiac Fibrosis and Remodeling
- Immunotherapy and Immune Responses
- RNA modifications and cancer
- Influenza Virus Research Studies
- Tissue Engineering and Regenerative Medicine
- Cancer, Hypoxia, and Metabolism
- SARS-CoV-2 detection and testing
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Viral Infections and Vectors
- Respiratory viral infections research
- Viral Infections and Outbreaks Research
- Pluripotent Stem Cells Research
- Monoclonal and Polyclonal Antibodies Research
- interferon and immune responses
- Mesenchymal stem cell research
- Long-Term Effects of COVID-19
- Cardiac Ischemia and Reperfusion
- Poxvirus research and outbreaks
- Hippo pathway signaling and YAP/TAZ
- Extracellular vesicles in disease
- vaccines and immunoinformatics approaches
Centro Nacional de Biotecnología
2019-2024
Consejo Superior de Investigaciones Científicas
2022-2024
Universidad Autónoma de Madrid
2019-2024
Hospital del Mar Research Institute
2018
Universitat Pompeu Fabra
2018
Current coronavirus disease-19 (COVID-19) vaccines are administered by the intramuscular route, but this vaccine administration failed to prevent severe acute respiratory syndrome 2 (SARS-CoV-2) virus infection in upper tract, mainly due absence of virus-specific mucosal immune responses. It is hypothesized that intranasal (IN) vaccination could induce both and systemic responses blocked SARS-CoV-2 transmission COVID-19 progression. Here, we evaluated mice IN three modified vaccinia Ankara...
Oxidative stress-induced myocardial apoptosis and necrosis are critically involved in ischemic infarction, several sources of extracellular vesicles appear to be enriched therapeutic activities. The central objective was identify validate the differential exosome miRNA repertoire human cardiac progenitor cells (CPC). CPC exosomes were first analyzed by LC-MS/MS compared RNAseq with exomes mesenchymal stromal fibroblasts define their (exo-miRSEL). Proteomics demonstrated a highly significant...
Williams-Beuren syndrome (WBS) is a neurodevelopmental disorder caused by heterozygous deletion of 26–28 genes at chromosome band 7q11.23. The complete (CD) mouse model mimics the most common found in WBS patients and recapitulates neurologic features along with some cardiovascular manifestations leading to significant cardiac hypertrophy increased cardiomyocytes' size. Epigallocatechin-3-gallate (EGCG), abundant catechin green tea, has been associated potential health benefits, both on...
The development of novel optimized vaccines against coronavirus disease 2019 (COVID-19) that are capable controlling the severe acute respiratory syndrome 2 (SARS-CoV-2) pandemic and appearance different variants concern (VoC) is needed to fully prevent transmission virus. In present study, we describe enhanced immunogenicity efficacy elicited in hamsters by a modified vaccinia virus Ankara (MVA) vector expressing full-length prefusion-stabilized SARS-CoV-2 spike (S) protein [termed...
Despite the decrease in mortality and morbidity due to SARS-CoV-2 infection, incidence of infections Omicron subvariants remains high. The mutations acquired by these subvariants, mainly concentrated receptor-binding domain (RBD), have caused a shift infectivity transmissibility, leading loss effectiveness first authorized COVID-19 vaccines, among other reasons, neutralizing antibody evasion. Hence, generation new vaccine candidates adapted is special interest an effort overcome this immune...
The constant appearance of new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants concern (VoCs) has jeopardized the protective capacity approved vaccines against disease-19 (COVID-19). For this reason, generation vaccine candidates adapted to emerging VoCs is special importance. Here, we developed an optimized COVID-19 candidate using modified vaccinia virus Ankara (MVA) vector express a full-length prefusion-stabilized SARS-CoV-2 spike (S) protein, containing 3 proline...
Human cardiac progenitor cells (hCPC) are considered a good candidate in cell therapy for ischemic heart disease, demonstrating capacity to improve functional recovery after myocardial infarction (MI), both small and large preclinical animal models. However, improvements required terms of engraftment efficacy. Based on previously published reports, insulin-growth factor 1 (IGF-1) hepatocyte growth (HGF) have demonstrated substantial cardioprotective, repair regeneration activities, so they...
Current vaccines against SARS-CoV-2, based on the original Wuhan sequence, induce antibodies with different degrees of cross-recognition new viral variants concern. Despite potent responses generated in vaccinated and infected individuals, Omicron (B.1.1.529) variant causes breakthrough infections, facilitating transmission. We previously reported a vaccine cyclic peptide containing 446-488 S1 sequence (446-488cc) SARS-CoV-2 spike (S) protein from isolate. To provide best immunity Omicron,...
Adult progenitor cells reside in specialized microenvironments which maintain their undifferentiated cell state and trigger regenerative responses following injury. Although these environments are well described several tissues, the cellular components that comprise cardiac environment where located remain unknown. Here we use Bmi1CreERT Bmi1GFP mice as genetic tools to trace damage-responsive throughout mouse lifespan. In adolescent mice, Bmi1+ broadly distributed myocardium. adult however,...
Abstract Administration of neutralizing antibodies (nAbs) has proved to be effective by providing immediate protection against SARS‐CoV‐2. However, dual strategies combining virus neutralization and immune response stimulation enhance specific cytotoxic T cell responses, such as dendritic (DC) cross‐priming, represent a promising field but have not yet been explored. Here, broadly nAb, TN , are first generated grafting an anti‐RBD biparatopic tandem nanobody onto trimerbody scaffold. Cryo‐EM...
Unravelling the molecular mechanism of COVID-19 vaccines through transcriptomic pathways involved in host response to SARS-CoV-2 infection is key understand how work, and for development optimized that can prevent emergence variants concern (VoCs) future outbreaks. In this study, we investigated effects vaccination with a modified vaccinia virus Ankara (MVA)-based vector expressing full-length spike protein (MVA-S) on lung transcriptome from susceptible K18-hACE2 mice after infection. One...
Abstract Clinical trials evaluating cardiac progenitor cells (CPC) demonstrated feasibility and safety, but no clear functional benefits. Therefore a deeper understanding of CPC biology is warranted to inform strategies capable enhance their therapeutic potential. Here we have defined, using label-free proteomic approach, the differential cytoplasmic nuclear compartments human (hCPC). Global analysis repertoire in hCPC suggested an important hypoxia response capacity active collagen...
The adult mammalian heart has been demonstrated to be endowed with low but real turnover capacity, especially for cardiomyocytes, the key functional cell type. source, however, of that capacity remains controversial. In this regard, we have defined and characterized a resident multipotent cardiac mouse progenitor population, Bmi1+DR (for Bmi1+ Damage-Responsive cells). is one types lowest ROS (Reactive Oxygen Species) levels in heart, being particularly by their close relationship vessels,...
Abstract Clinical trials evaluating cardiac progenitor cells (CPC) demonstrated feasibility and safety, but no clear functional benefits. Therefore a deeper understanding of CPC biology is warranted to inform strategies capable enhance their therapeutic potential. Here we have defined, using label-free proteomic approach, the differential cytoplasmic nuclear compartments human (hCPC). Global analysis repertoire in hCPC suggested an important hypoxia response capacity active collagen...