Permeability alterations of microvascular endothelia may be a factor in the plasma leakage produced by dengue virus infection. Confluent monolayers human dermal endothelial cell line HMEC-1 were utilized as an experimental model to study cellular responses induced virus. Infected showed increased permeability for [(3)H]mannitol, but no changes observed 4-70 kDa dextrans at 48 h post-infection (p.i.), time which viral titres reached maximal values and 40 % cells expressed proteins. A further...

Endothelial cells (ECs) represent the major component of embryonic pancreatic niche and play a key role in differentiation insulin-producing β vivo. However, it is unknown if ECs promote such vitro. We investigated whether interaction with mouse embryoid bodies (EBs) culture promotes progenitors mechanisms involved. developed co-culture system EBs human microvascular (HMECs). An increase expression markers PDX-1, Ngn3, Nkx6.1, proinsulin, GLUT-2, Ptf1a was observed at interface between...

Biliary epithelia express high levels of CD44 in hepatobiliary diseases. The role CD44-hyaluronic acid interaction biliary pathology, however, is unclear. A rat model hepatic cholestasis induced by bile duct ligation was employed for characterization expression and extracellular hyaluronan distribution. Cell culture experiments were to determine whether can regulate cholangiocyte growth through interacting with adhesion molecule CD44. epithelial cells found the highest level mRNA among four...

Endothelial cells (ECs) play an essential role in pancreatic organogenesis. We hypothesise that effective vitro interactions between human microvascular endothelial (HMECs) and pluripotent stem (hPSCs) results the generation of functional beta cells. Embryoid bodies (EBs) derived from hPSCs were cultured alone (controls) or with ECs collagen gels. Subsequently, analysed for cell markers, then isolated expanded. Insulin secretion response to glucose was evaluated by static dynamic...

Angiogenesis is a dynamic process that requires an interaction of pro-and antiangiogenic factors. It known the cytokine leptin stimulates endothelial cell growth and angiogenesis, but further quantitative analysis necessary to understand angiogenic effects. The quail chorioallantoic membrane (CAM) assay has been used study angiogenesis in vivo by focusing on morphometric parameters quantify vascular complexity density. We activity using CAM digital morphometry computer-assisted image...

Abstract Here we document three highly reproducible protocols: (1) a culture system for the derivation of human oligodendrocytes (OLs) from induced pluripotent stem cells (hiPS) and their further maturation—our protocol generates viral‐ integration‐free OLs that efficiently commit move forward in OL lineage, recapitulating all steps known to occur during vivo development; (2) method isolation, propagation maintenance neural (NSCs); (3) production, perinatal rodent brain‐derived NSCs. Our...

Here we document three highly reproducible protocols: (1) a culture system for the derivation of human oligodendrocytes (OLs) from induced pluripotent stem cells (hiPS) and their further maturation-our protocol generates viral- integration-free OLs that efficiently commit move forward in OL lineage, recapitulating all steps known to occur during vivo development; (2) method isolation, propagation maintenance neural (NSCs); (3) production, perinatal rodent brain-derived NSCs. Our unique...

Endothelial cells (ECs) provide inductive signals for cell differentiation in vivo. However, it is unknown if these promote such vitro and the involved. We investigated whether ECs are able to enhance of three germ layers underlying mechanisms. established a coculture system mouse embryoid bodies (EBs) ECs. Then, we analyzed expression markers representative layers, as PDX-1, proinsulin, insulin1 (endoderm), nestin, neurofilament light (ectoderm), CD31, cardiotin, cardiac troponin I...

Avian chorioallantoic membrane (CAM) has been used as a model to explore angiogenesis and study the microvasculature of transplanted tissues. Because CAM provides vascular bed, cells can be implanted, their development monitored modified. We differentiation process embryoid bodies (EBs) derived from mouse embryonic stem (ESCs) influenced by bed. After EBs were incubated in for 5 days, they underwent further became tissue masses (TMs) different morphologies those that grew outside CAM....

Background: Lichen Sclerosus (LS) is a debilitating disease that causes chronic inflammation most commonly noted in the anogenital region.Many patients with genital report unsuccessful results standard treatment options including steroids, calcineurin inhibitors, and/or hormone therapy.These presented to our clinic seeking autologous adipose-derived stem cell (AASCT).Methods: Data was collected through questionnaires regarding symptoms on daily basis and quality of life...

Liver transplantation is the option for end‐stage liver diseases. Shortage of donor organ a major issue. To overcome this, alternative approaches including hepatic cell transplantation, bioartificial devise and whole engineering are being investigated. We focus on developing engineered using natural scaffold generated via decellularization process. The would maintain authentic three‐ dimensional micro‐architecture vasculature. repopulated with patient‐specific cells differentiated from...

We introduce a novel node-structural map method for digital image-based analysis of angiogenesis, and apply it to quantitate the effect pro-angiogenic (e.g. VEGF) anti-angiogenic anti-VEGF) treatments on blood vessel patterns in quail chorioallantoic membrane (CAM) <i>in vivo</i> model. After segmentation skeletonization, is derived represent structural property each pixel skeletonized image, such as end nodes (root leaf), branch furcation/junction nodes. By combining with vascular thickness...