A5070367677- SARS-CoV-2 and COVID-19 Research
- Mosquito-borne diseases and control
- Hepatitis C virus research
- COVID-19 Clinical Research Studies
- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
- Viral Infections and Immunology Research
- interferon and immune responses
- Viral Infections and Vectors
- Hepatitis B Virus Studies
- Virology and Viral Diseases
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- HIV Research and Treatment
- Long-Term Effects of COVID-19
- Respiratory viral infections research
- Adipose Tissue and Metabolism
- Viral-associated cancers and disorders
- Hippo pathway signaling and YAP/TAZ
- Retinal and Optic Conditions
- Immune Cell Function and Interaction
- Heat shock proteins research
- CAR-T cell therapy research
- Insect symbiosis and bacterial influences
- Invertebrate Taxonomy and Ecology
University of California, Los Angeles
2016-2025
Broad Center
2019-2024
California NanoSystems Institute
2021-2024
UCLA Jonsson Comprehensive Cancer Center
2020-2024
Cedars-Sinai Medical Center
2012-2023
UCLA Health
2023
UCLA Medical Center
2023
Regenerative Medicine Institute
2014-2016
California Institute for Regenerative Medicine
2013
University of Arkansas at Fayetteville
2003-2009
Coronavirus disease 2019 (COVID-19) is a pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). COVID-19 defined symptoms, but cardiac complications including viral myocarditis are also prevalent. Although ischemic and inflammatory responses can detrimentally affect function, the direct impact of SARS-CoV-2 infection on human cardiomyocytes not well understood. Here, we utilize induced pluripotent stem cell-derived (hiPSC-CMs) as model to examine mechanisms...
Syndrome coronavirus 2 (SARS-CoV-2) infectious virions are viable on various surfaces (e.g., plastic, metals, and cardboard) for several hours. This presents a transmission cycle human infection that can be broken by developing new inactivation approaches. We employed an efficient cold atmospheric plasma (CAP) with argon feed gas to inactivate SARS-CoV-2 including metal, cardboard, basketball composite leather, football baseball leather. These results demonstrate the great potential of CAP...
Abstract Viruses hijack host cell metabolism to acquire the building blocks required for replication. Understanding how SARS-CoV-2 alters may lead potential treatments COVID-19. Here we profile metabolic changes conferred by infection in kidney epithelial cells and lung air-liquid interface (ALI) cultures, show that increases glucose carbon entry into TCA cycle via increased pyruvate carboxylase expression. also reduces oxidative glutamine while maintaining reductive carboxylation....
The hepatitis C viral (HCV) genome is translated through an internal ribosome entry site (IRES) as a single polyprotein precursor that subsequently cleaved into individual mature proteins. Nonstructural protein 5A (NS5A) one of these proteins has been implicated in regulation replication, translation from the IRES and packaging. We sought to identify cellular interact with NS5A determine whether interactions may play role production. Mass spectrometric analysis coimmunoprecipitated complexes...
SARS-CoV-2 has currently precipitated the COVID-19 global health crisis. We developed a medium-throughput drug-screening system and identified small-molecule library of 34 430 protein kinase inhibitors that were capable inhibiting cytopathic effect in human epithelial cells. These drug are various stages clinical trials. detected key proteins involved cellular signaling pathways mTOR-PI3K-AKT, ABL-BCR/MAPK, DNA-damage response critical for infection. A drug-protein interaction-based...
Abstract Viruses are known to perturb host cellular metabolism enable their replication and spread. However, little is about the interactions between Zika virus (ZIKV) infection metabolism. Using primary human retinal vascular endothelial cells an established cell line, we investigated role of AMP-activated protein kinase (AMPK), a master regulator energy metabolism, in response ZIKV challenge. caused time-dependent reduction active phosphorylated state AMPK its downstream target acetyl-CoA...
Extrapulmonary manifestations of COVID-19 are associated with a much higher mortality rate than pulmonary manifestations. However, little is known about the pathogenesis systemic complications COVID-19. Here, we create murine model SARS-CoV-2–induced severe toxicity and multiorgan involvement by expressing human ACE2 transgene in multiple tissues via viral delivery, followed administration SARS-CoV-2. The animals develop profound phenotype within 7 days weight loss, morbidity, failure to...
ABSTRACT Novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreak in the city of Wuhan, China during December 2019, has now spread to various countries across globe triggering a heightened containment effort. This human pathogen is member betacoronavirus genus carrying 30 kilobase single positive-sense RNA genome. Understanding evolution, zoonotic transmission, and source this novel virus would help accelerating prevention efforts. The present study reported detailed...
Significance COVID-19 is taking a major toll on personal health, healthcare systems, and the global economy. With three betacoronavirus epidemics in less than 20 y, there an urgent need for therapies to combat new existing coronavirus outbreaks. Our analysis of clinical data from over 300,000 patients health systems demonstrates 50% reduced risk lithium, direct inhibitor glycogen synthase kinase-3 (GSK-3). We further show that GSK-3 essential phosphorylation SARS-CoV-2 nucleocapsid protein...
Coronavirus disease 2019 (COVID-19) is the latest respiratory pandemic resulting from zoonotic transmission of severe acute syndrome-related coronavirus 2 (SARS-CoV-2). Severe symptoms include viral pneumonia secondary to infection and inflammation lower tract, in some cases causing death. We developed primary human lung epithelial models understand responses proximal distal epithelium SARS-CoV-2 infection. Differentiated air-liquid interface cultures airway 3D organoid alveolar were readily...
COVID-19 pandemic has infected more than 154 million people worldwide and caused 3.2 deaths. It is transmitted by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) affects respiratory tract as well extra-pulmonary systems, including pancreas, that express virus entry receptor, Angiotensin-Converting Enzyme (ACE2) receptor. Importantly, endocrine exocrine latter composed of ductal acinar cells, high levels ACE2, which correlates to impaired functionality characterized acute...
RNA viruses continue to remain a threat for potential pandemics due their rapid evolution. Potentiating host antiviral pathways prevent or limit viral infections is promising strategy. Thus, by testing library of innate immune agonists targeting pathogen recognition receptors, we observe that Toll-like receptor 3 (TLR3), stimulator interferon genes (STING), TLR8, and Dectin-1 ligands inhibit arboviruses, Chikungunya virus (CHIKV), West Nile virus, Zika varying degrees. STING (cAIMP, diABZI,...
The human transmembrane 6 superfamily member 2 (TM6SF2) gene has been implicated in plasma lipoprotein metabolism, alcoholic and non-alcoholic fatty liver disease myocardial infarction multiple genome-wide association studies. To investigate the role of Tm6sf2 metabolic homeostasis, we generated mice with elevated expression using adeno-associated virus (AAV)-mediated delivery. Hepatic overexpression mouse resulted phenotypes previously observed Tm6sf2-deficient including reduced lipid...
: media-1vid110.1542/5804915134001PEDS-VA_2018-1104Video Abstract OBJECTIVES: To characterize ophthalmic manifestations of confirmed or suspected antenatal Zika virus (ZIKV) exposure.Infants with ZIKV exposure were referred for evaluation during the 2015-2016 Rio de Janeiro outbreak. Mothers symptomatic infection pregnancy and/or infants microcephaly other findings that suggestive tested reverse transcriptase polymerase chain reaction (RT-PCR). Complete eye examinations performed by...
SUMMARY Coronavirus disease 2019 (COVID-19) is a viral pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). COVID-19 predominantly defined symptoms, but cardiac complications including arrhythmias, heart failure, and myocarditis are also prevalent. Although systemic ischemic inflammatory responses can detrimentally affect function, direct impact of SARS-CoV-2 infection on human cardiomyocytes not well-understood. We used induced pluripotent stem cell-derived...
Abstract The transcriptome of SARS-CoV-2-infected cells that reflects the interplay between host and virus has provided valuable insights into mechanisms underlying SARS-CoV-2 infection COVID-19 disease progression. In this study, we show can establish a robust in HEK293T overexpress human angiotensin-converting enzyme 2 (hACE2) without triggering significant immune response. Instead, endoplasmic reticulum stress unfolded protein response-related pathways are predominantly activated. By...