A5084360095- Pluripotent Stem Cells Research
- Neonatal Respiratory Health Research
- Renal and related cancers
- Tissue Engineering and Regenerative Medicine
- Hippo pathway signaling and YAP/TAZ
- SARS-CoV-2 and COVID-19 Research
- Reproductive Biology and Fertility
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- COVID-19 Clinical Research Studies
- Congenital heart defects research
- Congenital Diaphragmatic Hernia Studies
- Developmental Biology and Gene Regulation
- Medical Imaging and Pathology Studies
- CRISPR and Genetic Engineering
- Cardiac Fibrosis and Remodeling
- Zebrafish Biomedical Research Applications
- Digestive system and related health
- Monoclonal and Polyclonal Antibodies Research
- Cardiac Structural Anomalies and Repair
- Genomics and Chromatin Dynamics
- AI in cancer detection
- Genetic factors in colorectal cancer
- Energy Harvesting in Wireless Networks
- Epigenetics and DNA Methylation
- Long-Term Effects of COVID-19
Harvard Stem Cell Institute
2020-2022
Harvard University
2019-2022
Boston Children's Hospital
2019-2022
Spanish National Centre for Cardiovascular Research
2017-2022
Boston University
2019
Universidad Autónoma de Madrid
2018
In the zebrafish (
Coronavirus disease 2019 (COVID-19) is the latest respiratory pandemic resulting from zoonotic transmission of severe acute syndrome-related coronavirus 2 (SARS-CoV-2). Severe symptoms include viral pneumonia secondary to infection and inflammation lower tract, in some cases causing death. We developed primary human lung epithelial models understand responses proximal distal epithelium SARS-CoV-2 infection. Differentiated air-liquid interface cultures airway 3D organoid alveolar were readily...
Type 2 alveolar epithelial cells (AT2s), facultative progenitor of the lung alveolus, play a vital role in biology distal lung. In vitro model systems that incorporate human cells, recapitulate primary AT2s, and interface with outside environment could serve as useful tools to elucidate functional characteristics AT2s homeostasis disease. We others recently adapted induced pluripotent stem cell–derived (iAT2s) for air-liquid (ALI) culture. Here, we comprehensively characterize effects ALI...
The Notch signalling pathway plays fundamental roles in diverse developmental processes metazoans, where it is important driving cell fate and directing differentiation of various types. However, we still have limited knowledge about the role early preimplantation stages mammalian development, or how interacts with other pathways active at these such as Hippo. By using genetic pharmacological tools vivo, together image analysis single embryos pluripotent culture, found that from 4-cell...
Abstract A well-tolerated and cost-effective oral drug that blocks SARS-CoV-2 growth dissemination would be a major advance in the global effort to reduce COVID-19 morbidity mortality. Here, we show FDA-approved nitazoxanide (NTZ) significantly inhibits viral replication infection different primate human cell models including stem cell-derived alveolar epithelial type 2 cells. Furthermore, NTZ synergizes with remdesivir, it broadly of variants B.1.351 (beta), P.1 (gamma), B.1617.2 (delta)...
Pluripotent cells are a transient population of the mammalian embryo dependent on transcription factors, such as OCT4 and NANOG, which maintain pluripotency while suppressing lineage specification. However, these factors also expressed during early phases differentiation, their role in transition from to specification is largely unknown. We found that play dual regulating key specifiers, initially repressing expression later being required for proper activation. show Oct4 necessary...
ABSTRACT Pluripotency is regulated by a network of transcription factors that maintain early embryonic cells in an undifferentiated state while allowing them to proliferate. NANOG critical factor for maintaining pluripotency and its role primordial germ cell differentiation has been well described. However, Nanog expressed during gastrulation across all the posterior epiblast, only later development expression restricted cells. In this work, we unveiled previously unknown mechanism which...
Article27 February 2019Open Access Transparent process The pluripotency factor NANOG controls primitive hematopoiesis and directly regulates Tal1 Julio Sainz de Aja Centro Nacional Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain Search for more papers by this author Sergio Menchero Isabel Rollan Antonio Barral Maria Tiana Wajid Jawaid Wellcome-Medical Research Council Cambridge Stem Cell Institute, Cambridge, UK Department of Haematology, Institute Medical Research,...
ABSTRACT In the zebrafish ( Danio rerio ), regeneration and fibrosis after cardiac injury are not mutually exclusive responses. Upon cryoinjury, collagen other extracellular matrix (ECM) proteins accumulate at site. However, in contrast to situation mammals, is transient its regression concomitant with regrowth of myocardial wall. Little known about cells producing this fibrotic tissue or how it resolves. Using novel genetic tools mark periostin b- 1alpha2 col1a2 )-expressing combination...
Abstract The Notch signalling pathway plays fundamental roles in diverse developmental processes metazoans, where it is important driving cell fate and directing differentiation of various types. However, we still have limited knowledge about the role early preimplantation stages mammalian development, or how interacts with other pathways active at these such as Hippo. By using genetic pharmacological tools vivo, together image analysis single embryos pluripotent culture, found that from...
ABSTRACT Pluripotent cells are a transient population present in the early mammalian embryo dependent on transcription factors, such as OCT4 and NANOG, which maintain pluripotency while simultaneously suppressing lineage specification. Interestingly, these factors not exclusive to uncommitted cells, but also expressed during phases of differentiation. However, their role transition from specification is largely unknown. Using genetic models for controlled Oct4 or Nanog expression...
ABSTRACT Pluripotency is regulated by a network of transcription factors that maintains early embryonic cells in an undifferentiated state while allowing them to proliferate. NANOG critical factor for maintaining pluripotency and its role primordial germ cell differentiation has been well described. However, Nanog expressed during gastrulation across all the posterior epiblast, only later development expression restricted cells. In this work, we unveiled previously unknown mechanism which...
Abstract We here present COOBoostR ( https://github.com/SWJ9385/COOBoostR ), a computational method designed for the putative prediction of tissue-or cell-of-origin various cancer types. leverages regional somatic mutation density information and chromatin mark features to be applied an extreme gradient boosting-based machine-learning algorithm. ranks marks from tissue cell types which best explain landscape any sample interest. Through integrating either ChIP-seq based data or bulk/single...