A5082596943- Skin and Cellular Biology Research
- Immunotherapy and Immune Responses
- Cervical Cancer and HPV Research
- Virus-based gene therapy research
- Wound Healing and Treatments
- Immune Cell Function and Interaction
- Bone and Dental Protein Studies
- Barrier Structure and Function Studies
- Reproductive System and Pregnancy
- Herpesvirus Infections and Treatments
- Helicobacter pylori-related gastroenterology studies
- Estrogen and related hormone effects
- Veterinary medicine and infectious diseases
- Cytokine Signaling Pathways and Interactions
- Ion Transport and Channel Regulation
- Transgenic Plants and Applications
- Heme Oxygenase-1 and Carbon Monoxide
- Genital Health and Disease
- Wnt/β-catenin signaling in development and cancer
- Connexins and lens biology
- Tendon Structure and Treatment
- Galectins and Cancer Biology
- Poxvirus research and outbreaks
- Autophagy in Disease and Therapy
- S100 Proteins and Annexins
Tecnológico de Monterrey
2023-2024
Universidad México Contemporáneo
2023
Hospital Juárez de México
2011-2021
Instituto Politécnico Nacional
2008-2020
Universidad Nacional Autónoma de México
2013
Georgetown University Medical Center
2011
Georgetown University
2011
University of Houston
2011
University of Wisconsin–Madison
2011
Epidermal Growth Factor (EGF) is a key regulator of epithelial paracellular permeability, property that depends on tight junctions (TJ) and can be evaluated through the measurement transepithelial electrical resistance (TER). EGF increases TER MDCK monolayers by inducing ERK1/2-dependent downregulation claudin-2 (CLDN-2) upregulation claudin-4 (CLDN-4). Because either increments or decrements in often involve Src activation cell differentiation occasionally STAT3, here we investigated...
Abstract The contribution of the Wnt signaling pathway to human papilloma virus (HPV)-induced carcinogenesis is poorly understood. In high-grade dysplastic lesions that are caused by high-risk HPVs (HR-HPV), β-catenin often located in cell nucleus, which suggests may be involved development HPV-related carcinomas. Most oncogenic potential HR-HPVs resides on PDZ-binding domain E6 protein. We hypothesized HPV16-E6 oncoprotein induces nuclear accumulation due its capacity degrade PDZ-containing...
Na+, K+-ATPase, or the Na+ pump, is a key component in maintenance of epithelial phenotype. In most epithelia, pump located basolateral domain. Studies from our laboratory have shown that β1 subunit K+-ATPase plays an important role this mechanism because homotypic β1-β1 interactions between neighboring cells stabilize lateral membrane. However, retinal pigment epithelium (RPE), apical The polarization unclear. We hypothesized RPE depends on expression its β2 subunit. ARPE-19 cultured for up...
Persistent infection with Helicobacter pylori (H. pylori) is an important factor in gastric diseases. The vacA and cagA virulence factors of H. contribute to the development these Triple therapy containing clarithromycin has been used eradicate this infection. Unfortunately, resistance antibiotic primary cause treatment failure. This study aimed determine prevalence resistance-associated mutations assess relationship between Mexican patients infected pylori. genotypes were determined by...
Two siblings from a Mexican family who carried lethal Raine syndrome are presented. A newborn term male (case 1) and his 21 gestational week brother 2), with similar osteosclerotic pattern: generalized osteosclerosis, which is more evident in facial bones cranial base. Prenatal findings at weeks histopathological features for case 2 described. novel combination of biallelic FAM20C pathogenic variants were detected, maternal cytosine duplication position 456 paternal deletion 474 exon 1,...
Although high-risk human papillomavirus (HR‑HPV) infection has a prominent role in the aetiology of cervical cancer (CC), sex steroid hormones may also be involved this process; however, cooperation between oestrogen and HR‑HPV early stages carcinogenesis is poorly understood. Since 17β-oestradiol (E2) HPV type 16‑E7 oncoprotein induce CC transgenic mice, microarray analysis was performed present study to generate global gene expression profiles from 2‑month‑old FVB (non‑transgenic) K14E7...
According to their oncogenic properties, Human Papillomaviruses (HPVs) are classified into two types: Low-Risk (LR-HPVs) and High-Risk (HR-HPVs). The immune system naturally controls the majority of HPV infections; however, when HR-HPV infection is persistent, risk developing cervical cancer increases. Previous studies indicate that multiple-infection or coinfection with occurs frequently can potentiate development lesions. This study aimed establish rate in squamous intraepithelial lesions...
The aim of this work was to compare the early gene expression profiles in skin HPV16-E6 transgenic mice regulated by E6 PDZ-binding motif.The global transcriptional dorsal biopsies from K14E6 and K14E6Δ146-151 were compared using microarrays. Relevant genes obtained most differentially expressed processes further examined RT-qPCR, situ RT-PCR, Western blot, or immunofluorescence.The transcriptomic landscape versus shows that affected those related keratinocyte differentiation, stem cell...
Objective. The aim of this study was to analyze the effects HPV16 E7 oncoprotein on dendritic cells (DCs) and CD11b + Gr1 using K14E7 transgenic mouse model. Materials Methods. morphology DCs analyzed in male skin epidermal sheets immunofluorescence confocal microscopy. Flow cytometry used determine percentages different tissues evaluate migration DCs. Results. In model, Langerhans migratory activity were abnormal. An increase observed blood mice, molecules related chemoattraction (MCP1...
This work aimed to determine if cataractous changes associated with EMT occurring in the K14E6 mice lenses are TGF-β and Wnt/β-catenin signaling activation.Cataracts of were analysed histologically; components evaluated by Western blot, RT-qPCR, situ RT-PCR, IHC, or IF technics. Metalloproteinases involved also assayed using zymography. The endogenous stabilisation Smad7 protein was assessed an HDAC inhibitor.The mice, which displayed binocular cataracts 100% animals, exhibited loss tissue...
FAM20C (family with sequence similarity 20, member C) is a serine/threonine-specific protein kinase that ubiquitously expressed and mainly associated biomineralization phosphatemia regulation. It mostly known due to pathogenic variants causing its deficiency, which results in Raine syndrome (RNS), sclerosing bone dysplasia hypophosphatemia. The phenotype recognized by the skeletal features, are related hypophosphorylation of different bone-target proteins. However, has many targets,...
Epithelial cells transport substances through the cellular and paracellular pathways. The last one depends on tight junctions, particularly claudins, family of integral membrane proteins responsible for permeability selectivity these junctions. 300 nM ouabain (OUA) induces endocytosis lysosomal degradation claudin-2 -4 in an Src ERK1/2 kinases-dependent manner. Here we investigate whether OUA-induced claudins implicates autophagy renal epithelial Madin-Darby canine kidney cells. During...
REVISIÓN ResumenEl cáncer se clasifica como esporádico, familiar y hereditario.El hereditario comprende el 5-10% de todos los casos.Es un grupo enfermedades causadas por variantes patogénicas genes involucrados en varios procesos biológicos, asociadas con riesgo desarrollar tumores.Dependiendo del patrón tumores miembros las familias afectadas, conocen diferentes síndromes hereditario, síndrome mama-ovario Lynch la neoplasia endocrina múltiple 1. Hasta fecha han identificado aproximadamente...