Alfredo Hidalgo‐Miranda
A5004575782- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- MicroRNA in disease regulation
- Cancer Genomics and Diagnostics
- Circular RNAs in diseases
- Cervical Cancer and HPV Research
- SARS-CoV-2 and COVID-19 Research
- Acute Lymphoblastic Leukemia research
- RNA Research and Splicing
- SARS-CoV-2 detection and testing
- Childhood Cancer Survivors' Quality of Life
- COVID-19 Clinical Research Studies
- Nutrition, Genetics, and Disease
- Molecular Biology Techniques and Applications
- Genomics and Chromatin Dynamics
- Genetic Associations and Epidemiology
- interferon and immune responses
- Hematopoietic Stem Cell Transplantation
- BRCA gene mutations in cancer
- Immunotherapy and Immune Responses
- HER2/EGFR in Cancer Research
- Cancer Cells and Metastasis
- Cancer-related Molecular Pathways
- Acute Myeloid Leukemia Research
- Immune Cell Function and Interaction
National Institute of Genomic Medicine
2015-2024
Instituto de Medicina Genómica
2008-2022
Universidad Nacional Autónoma de México
2017
GTx (United States)
2015
Cancer Genomics Centre
2013-2014
Hospital Central Militar
2012
Pemex (Mexico)
2012
Tecnológico de Monterrey
2007
Centro Medico Nacional Siglo XXI
1998-2006
Mexican Social Security Institute
2001-2005
Head and neck squamous cell carcinoma (HNSCC) is a common, morbid, frequently lethal malignancy. To uncover its mutational spectrum, we analyzed whole-exome sequencing data from 74 tumor-normal pairs. The majority exhibited profile consistent with tobacco exposure; human papillomavirus was detectable by DNA infected tumors. In addition to identifying previously known HNSCC genes (TP53, CDKN2A, PTEN, PIK3CA, HRAS), our analysis revealed many not implicated in this At least 30% of cases...
Breast carcinoma is the leading cause of cancer-related mortality in women worldwide, with an estimated 1.38 million new cases and 458,000 deaths 2008 alone. This malignancy represents a heterogeneous group tumours characteristic molecular features, prognosis responses to available therapy. Recurrent somatic alterations breast cancer have been described, including mutations copy number alterations, notably ERBB2 amplifications, first successful therapy target defined by genomic aberration....
To gain insight into the genomic basis of diffuse large B-cell lymphoma (DLBCL), we performed massively parallel whole-exome sequencing 55 primary tumor samples from patients with DLBCL and matched normal tissue. We identified recurrent mutations in genes that are well known to be functionally relevant DLBCL, including MYD88 , CARD11 EZH2 CREBBP . also somatic for which a functional role has not been previously suspected. These include MEF2B MLL2 BTG1 GNA13 ACTB P2RY8 PCLO TNFRSF14 Further,...
Mexico harbors great cultural and ethnic diversity, yet fine-scale patterns of human genome-wide variation from this region remain largely uncharacterized. We studied genomic within over 1000 individuals representing 20 indigenous 11 mestizo populations. found striking genetic stratification among populations at varying degrees geographic isolation. Some groups were as differentiated Europeans are East Asians. Pre-Columbian substructure is recapitulated in the ancestry admixed across...
Mexico is developing the basis for genomic medicine to improve healthcare of its population. The extensive study genetic diversity and linkage disequilibrium structure different populations has made it possible develop tagging imputation strategies comprehensively analyze common variation in association studies complex diseases. We assessed benefit a Mexican haplotype map identification genes related diseases evaluated diversity, patterns, extent sharing using genomewide data from Mestizos...
Abstract Breast cancer is a heterogeneous pathology, but the genomic basis of its variability remains poorly understood in populations other than Caucasians. Here, through DNA and RNA portraits we explored molecular features breast cancers set Hispanic-Mexican (HM) women compared them to public multi-ancestry datasets. HM patients present an earlier onset disease, particularly aggressive clinical subtypes, non-Hispanic women. The age-related COSMIC signature 1 was more frequent those from...
Most individuals throughout the Americas are admixed descendants of Native American, European, and African ancestors. Complex historical factors have resulted in varying proportions ancestral contributions between within among ethnic groups. We developed a panel 446 ancestry informative markers (AIMs) optimized to estimate populations Latin America. used genome-wide data from 953 diverse African, American select AIMs for each three main continental that form basis modern populations....
Triple-negative breast cancers (TNBCs) are characterized by a poor prognosis and lack of targeted treatments, thus, new therapeutic strategies urgently needed. Inhibitors against programmed death-1 (PD-1)/PD-1 ligand (PD-L1) have shown significant efficacy in various solid cancers, but their activity TNBCs remains limited. Here, we report that human molecularly stratified for high levels PD-L1 (PD-L1High) showed significantly enriched expression immune cancer stemness pathways compared with...
It is known that cancer progresses by vertical gene transfer, but this paradigm ignores DNA circulates in higher organisms and it biologically active upon its uptake recipient cells. Here we confirm previous observations on the ability of cell-free to induce vitro cell transformation tumorigenesis treating NIH3T3 murine cells with serum colon patients supernatant SW480 human Cell did not occur if supernatants were depleted DNA. also demonstrated horizontal progression mediated circulating...
Objective To analyze a set of circulating microRNA (miRNA) in plasma from patients with primary Osteoarthritis (OA) and describe the biological significance altered miRNA OA based on an silico analysis their target genes. Methods expression was analyzed using TaqMan Low Density Arrays independent assays. The search for potential messenger RNA (mRNA) targets differentially expressed performed by means miRWalk miRecords database; we conducted relevance predicted pathway Reactome DAVID...
Abstract Triple-negative breast cancer (TNBC) is a heterogeneous and aggressive neoplasia lacking the expression of hormonal receptors human epidermal growth factor receptor-2. Accumulating evidence has highlighted importance miRNAs dysregulation in establishment programs, but functional role many remains unclear. The description roles might provide novel strategies for treatment. In present work, an integrated analysis miRNA transcriptional landscape was performed (N = 132), identifying...
Expression of the microRNA miR-21 has been found to be altered in almost all types cancers and it classified as an oncogenic or oncomir. Due critical functions its target proteins various signaling pathways, is attractive for genetic pharmacological modulation cancers. Cervical cancer second most common cause death from women worldwide persistent HPV infection main etiologic agent. This malignancy merits special attention development new treatment strategies. In present study we analyze role...
Abstract The molecular characterization of extracellular vesicles (EVs) has revealed a great heterogeneity in their composition at cellular and tissue level. Current isolation methods fail to efficiently separate EV subtypes for proteomic functional analysis. aim this study was develop reproducible scalable workflow increase the yield purity preparations. Through combination polymer‐based precipitation size exclusion chromatography (Pre‐SEC), we analyzed two subsets EVs based on CD9, CD63...
The aim of this study was to investigate the feasibility saliva sampling as a non-invasive and safer tool detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) compare its reproducibility sensitivity with nasopharyngeal swab samples (NPS). use sample pools also investigated.A total 2107 paired were collected from asymptomatic healthcare office workers in Mexico City. Sixty these analyzed two other independent laboratories for concordance analysis. Sample processing analysis...
Studies of pharmacogenomics-related traits are increasingly being performed to identify loci that affect either drug response or susceptibility adverse reactions. However, the effect polymorphisms can differ in magnitude be absent depending on population assessed. We used Affymetrix Drug Metabolizing Enzymes and Transporters (DMET) Plus array characterize distribution pharmacogenetics pharmacogenomics (PGx) relevance two samples from most populous Latin American countries, Brazil Mexico. The...
Abstract Deregulated expression of microRNAs has been associated with angiogenesis. Studying the miRNome locally advanced breast tumors we unsuspectedly found a dramatically repression miR-204, small non-coding RNA no previous involvement in tumor Downregulation miR-204 was confirmed an independent cohort patients and cancer cell lines. Gain-of-function analysis indicates that ectopic impairs proliferation, anchorage-independent growth, migration, invasion, formation 3D capillary networks...
Omicron is the most mutated SARS-CoV-2 variant—a factor that can affect transmissibility, disease severity, and immune evasiveness. Its genomic surveillance important in cities with millions of inhabitants an economic center, such as Mexico City. Results. From 16 November to 31 December 2021, we observed increase 88% prevalence We explored R346K substitution, prevalent 42% variants, known be associated escape by monoclonal antibodies. In a phylogenetic analysis, found several independent...