A5072785674- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Viral gastroenteritis research and epidemiology
- Cervical Cancer and HPV Research
- Virus-based gene therapy research
- Animal Virus Infections Studies
- T-cell and B-cell Immunology
- Influenza Virus Research Studies
- Viral Infections and Immunology Research
- Hepatitis Viruses Studies and Epidemiology
- Immune Response and Inflammation
- Monoclonal and Polyclonal Antibodies Research
- Toxin Mechanisms and Immunotoxins
- Respiratory viral infections research
- Cytokine Signaling Pathways and Interactions
- interferon and immune responses
- HIV Research and Treatment
- RNA and protein synthesis mechanisms
- Genital Health and Disease
- Infant Nutrition and Health
- Herpesvirus Infections and Treatments
- Cancer-related Molecular Pathways
- Transgenic Plants and Applications
- Galectins and Cancer Biology
- Brucella: diagnosis, epidemiology, treatment
Universidad Autónoma del Estado de Morelos
2015-2024
Universidad Autónoma del Estado de México
2015-2021
Universidad Nacional Autónoma de México
2000-2008
National Institute of Allergy and Infectious Diseases
1991-1995
Howard Hughes Medical Institute
1995
Yale University
1995
University of California, San Diego
1995
National Institutes of Health
1991-1993
National Cancer Institute
1991-1993
Consejo Nacional de Humanidades, Ciencias y Tecnologías
1988
Intracellular antigens must be processed before presentation to CD8+ T cells by major histocompatibility complex (MHC) class I molecules. Using a recombinant vaccinia virus (Vac) transiently express the Kd molecule, we studied antigen processing efficiency of 26 different human tumor lines. Three cell lines, all small lung carcinoma, consistently failed process endogenously synthesized proteins for Kd-restricted, Vac-specific cells. Pulse-chase experiments showed that MHC molecules were not...
RMA/S is a mutant cell line with decreased surface expression of major histocompatibility complex class I molecules that has been reported to be deficient in presenting endogenously synthesized influenza virus nucleoprotein (NP) cytotoxic T lymphocytes (CTL). In the present study we show cells can vesicular stomatitis nucleocapsid protein, and, under some conditions, NP, Kb-and Db-restricted CTL, respectively. Antigen presentation results from processing cytosolic pools proteins, and not...
Abstract MHC class I-restricted CTL play a central role in the immune response against methylcholanthrene (MCA)-induced sarcomas mice. We, therefore, hypothesized that MCA-induced tumors may evade recognition by failing to present Ag CD8+ CTL. Of number of previously described sarcomas, one, MCA 101, fails induce CTL, is nonimmunogenic, and grows rapidly lethally nonimmunosuppressed recipients. To better understand nonimmunogenicity 101 we examined its ability foreign Unlike immunogenic...
The transporter associated with antigen processing (TAP) transports short peptides from the cytosol to endoplasmic reticulum, where assemble class I molecules of major histocompatibility complex. TAP is comprised two subunits, termed TAP1 and TAP2. We produced recombinant vaccinia viruses that direct synthesis either individually or together. Virus-encoded rapidly efficiently assembled (t 5 min less) by cells does not spontaneously in detergent extracts. By confocal immunofluorescence...
Rotavirus (RV) is a common cause of severe gastroenteritis (GE) in children worldwide. Live oral RV vaccines protect against RVGE, but the immune correlates protection are not yet clearly defined. Inner capsid VP6 protein highly conserved, abundant, and immunogenic protein, VP6-specific mucosal antibodies, especially IgA, have been implicated to viral challenge mice. In present study systemic IgG IgA responses were induced by immunizing BALB/c mice intranasally with combination recombinant...
Live oral rotavirus (RV) vaccines are part of routine childhood immunization but associated with adverse effects, particularly intussusception. We have developed a non-live combined RV - norovirus (NoV) vaccine candidate consisting human inner-capsid rVP6 protein and NoV virus-like particles. To determine the effect delivery route on induction VP6-specific protective immunity, BALB/c mice were administered containing intramuscularly, intranasally or combination both, challenged murine RV. At...
This study used an in vivo mouse model to analyze the response of dendritic cells (DCs) Peyer's patches (PPs) within first 48 h infection with wild-type murine rotavirus EDIM (EDIMwt). After infection, absolute number DCs was increased by 2-fold PPs without a modification their relative percentage total cell number. Also, from infected mice showed time-dependent migration subepithelial dome (SED) and increase surface activation markers CD40, CD80, CD86. more evident at postinfection (p.i.)...
A number of different antigens have been successfully expressed in transgenic plants, and some are currently being evaluated as orally delivered vaccines. Here we report the successful expression rotavirus capsid proteins VP2 VP6 fruits tomato plants. By western blot analysis, using specific antibodies, determined that produced plants molecular weights similar to those found native rotavirus. The plant-synthesized protein retained capacity form trimers. We were able recover virus-like...
CD4+ T cells are major players in the immune response against several diseases; including AIDS, leishmaniasis, tuberculosis, influenza and cancer. Their activation has been successfully achieved by administering antigen coupled with antibodies, DC-specific receptors combination adjuvants. Unfortunately, most of adjuvants used so far experimental models unsuitable for human use. Therefore, DC-targeted vaccination awaits description potent, yet nontoxic The cholera B subunit (CTB) can be...
Inflammation and oxidative stress play major roles in endothelial dysfunction, are key factors the progression of cardiovascular diseases. The aim this study was to evaluate vitro effect three subfractions (SFs) from Cucumis sativus aqueous fraction reduce inflammatory induced by angiotensin II (Ang II) human microvascular cells-1 (HMEC-1) cells. cells were cultured with different concentrations Ang 0.08 or 10 μg/mL SF1, SF2, SF3, μmol losartan as a control. IL-6 (Interleukin 6)...
The infection caused by the influenza virus is a latent tret. limited access to vaccines and approved drugs highlights need for additional antiviral agents. Nucleozin its analogs have gain attention their promising anti-influenza activity. To contribute advancement of discovery design nucleozin analogs, we analyzed piperazine-modified increase conformational freedom. Also, describe new synthetic strategy obtain analogues, three molecules were synthesized two them biologically evaluated in...
In this work, we have studied the T-helper (Th)-cell response against rotavirus, in a mouse model. Adult BALB/c mice were inoculated parenterally with porcine rotavirus YM, and Th-cell from spleen cells virus two overlapping fragments of major capsid protein VP6 (VP6(1-192) VP6(171-397)) evaluated vitro. The Th recognized YM fragments, suggesting that there are at least epitopes on molecule. To study specificity clonal level, established hybridomas cross-reactive for strains SA11. Both...
ABSTRACT Oral immunization is a goal in vaccine development, particularly for pathogens that enter the host through mucosal system. This study was designed to explore immunogenic properties of Taenia crassiceps protective peptide GK-1 administered orally. Mice were orally immunized with synthetic its linear form or without Brucella lumazine synthase (BLS) protein adjuvant as chimera recombinantly bound BLS (BLS-GK-1). boosted twice only at 15-day intervals. A significant rate protection...