A5024580674- Neuroscience and Neuropharmacology Research
- Neurotransmitter Receptor Influence on Behavior
- Receptor Mechanisms and Signaling
- Parkinson's Disease Mechanisms and Treatments
- Nerve injury and regeneration
- Ion channel regulation and function
- Neural dynamics and brain function
- Neurological disorders and treatments
- Neurogenesis and neuroplasticity mechanisms
- Neuropeptides and Animal Physiology
- Neuroscience and Neural Engineering
- Cellular transport and secretion
- Neurobiology and Insect Physiology Research
- Nuclear Receptors and Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Photoreceptor and optogenetics research
- Gastrointestinal motility and disorders
- Memory and Neural Mechanisms
- Lipid Membrane Structure and Behavior
- Genetic Neurodegenerative Diseases
- Nicotinic Acetylcholine Receptors Study
- Gold and Silver Nanoparticles Synthesis and Applications
- Stress Responses and Cortisol
- Immunotherapy and Immune Responses
- Zebrafish Biomedical Research Applications
Hôpital Maisonneuve-Rosemont
2024
National Research Centre
2024
Université de Montréal
2015-2024
Montreal Clinical Research Institute
1992-2024
Research Network (United States)
2024
Center for Interdisciplinary Studies
2024
Jewish General Hospital
2016
Institute of Pharmacology
2012
Czech Academy of Sciences, Institute of Physiology
2008-2011
CNS Research (United States)
2011
Human mesenchymal stromal cells (MSC) have been shown to dampen immune response and promote tissue repair, but the underlying mechanisms are still under investigation. Herein, we demonstrate that umbilical cord-derived MSC (UC-MSC) alter phenotype function of monocyte-derived dendritic (DC) through lactate-mediated metabolic reprogramming. UC-MSC can secrete large quantities lactate and, when present during monocyte-to-DC differentiation, induce instead acquisition M2-macrophage features in...
Abstract Mitochondria play a crucial role in neuronal survival through efficient energy metabolism. In pathological conditions, mitochondrial stress leads to death, which is regulated by the anti-apoptotic BCL-2 family of proteins. MCL-1 an protein localized mitochondria either outer membrane (OM) or inner (Matrix), have distinct roles inhibiting apoptosis and promoting bioenergetics, respectively. While for Mcl1 well characterized, protective function Matrix remains poorly understood. Here,...
Parkinson's disease (PD) is a neurodegenerative disorder characterized by the loss of dopamine (DA) neurons in substantia nigra pars compacta (SNc). Rare genetic mutations genes such as Parkin, Pink1, DJ-1, α-synuclein, LRRK2 and GBA are found to be responsible for about 15% cases. A key unanswered question PD pathophysiology why would these mutations, impacting basic cellular processes mitochondrial function neurotransmission, lead selective degeneration SNc DA neurons? We previously showed...
Atherosclerosis, the major cause of myocardial infarction and stroke, results from converging inflammatory, metabolic, biomechanical factors. Arterial lesions form at sites low disturbed blood flow but are suppressed by high laminar shear stress (LSS) mainly via transcriptional induction anti-inflammatory transcription factor, Kruppel-like factor 2 (Klf2). We therefore performed a whole genome CRISPR-Cas9 screen to identify genes required for LSS Klf2. Subsequent mechanistic investigation...
Glial cell line-derived neurotrophic factor (GDNF) is known to promote the survival and differentiation of dopaminergic neurons midbrain. GDNF also causes an enhancement dopamine release by a mechanism which presently unclear. Using isolated rat ventral tegmental area in culture, we have tested hypothesis that regulates establishment functional properties synaptic terminals. Previous studies shown single culture can co-release glutamate addition dopamine, leading generation fast excitatory...
Abstract Dopamine neurons have been suggested to use glutamate as a cotransmitter. To identify the basis of such phenotype, we examined expression three recently identified vesicular transporters (VGLUT1‐3) in postnatal rat dopamine culture. We found that majority isolated express VGLUT2, but not VGLUT1 or 3. In comparison, serotonin only VGLUT3. Single‐cell RT‐PCR experiments confirmed presence VGLUT2 mRNA neurons. Arguing for phenotypic heterogeneity among axon terminals, find proportion...
The "One neuron-one neurotransmitter" concept has been challenged frequently during the last three decades, and coexistence of neurotransmitters in individual neurons is now regarded as a common phenomenon. functional significance neurotransmitter is, however, less well understood. Several studies have shown that subpopulation dopamine (DA) ventral tegmental area (VTA) expresses vesicular glutamate transporter 2 (VGLUT2) suggested to use cotransmitter. VTA project limbic structures including...
Abstract: Chondroitin sulfate is referred as a symptomatic slow‐acting drug for osteoarthritis because it improves articular function, and reduces joint swelling effusion. In addition, chondroitin prevents space narrowing of the knee. We hypothesized that anti‐inflammatory effect associated to decrease in activation mitogen‐activated protein kinases (MAPK) transcription factors nuclear factor‐κB (NF‐κB) activator protein‐1 (AP‐1). Cultured rabbit chondrocytes were stimulated with...
Mesencephalic dopamine (DA) neurons have been suggested to use glutamate as a cotransmitter. Here, we suggest mechanism for this form of cotransmission by showing that subset DA both in vitro and vivo expresses vesicular transporter 2 (VGluT2). Expression VGluT2 decreases with age. Moreover, when are grown isolation using microculture system, there is marked upregulation expression. We provide evidence expression normally repressed through contact-dependent interaction GABA other neurons,...
The mesostriatal dopamine (DA) system contributes to several aspects of responses rewarding substances and is implicated in conditions such as drug addiction eating disorders. A subset DA neurons has been shown express the type 2 Vesicular glutamate transporter ( Vglut2 ) may therefore corelease glutamate. In present study, we analyzed mice with a conditional deletion f/f;DAT-Cre address functional significance glutamate–DA cophenotype for cocaine food reinforcement. Biochemical parameters...
The striatum is predominantly composed of medium spiny neurons (MSNs) that send their axons along two parallel pathways known as the direct and indirect pathways. MSNs from pathway express high levels D1 dopamine receptors, while D2 receptors. There has been much debate over extent colocalization these major receptors in adult animals. In addition, ontogeny segregation process never investigated. this paper, we crossed bacterial artificial chromosome drd1a-tdTomato drd2-GFP reporter...
Dopamine (DA) release in the CNS is critical for motor control and motivated behaviors. Dysfunction of its regulation thought to be implicated drug abuse diseases such as schizophrenia Parkinson's. Although various potassium channels located somatodendritic compartment DA neurons G-protein-gated inward rectifying (GIRK) have been shown regulate cell firing release, little presently known about role localized axon terminals these neurons. Here we used fast-scan cyclic voltammetry study...