A5026567475- Bacterial Genetics and Biotechnology
- Circadian rhythm and melatonin
- Escherichia coli research studies
- Salmonella and Campylobacter epidemiology
- Antimicrobial Peptides and Activities
- Sleep and Wakefulness Research
- Probiotics and Fermented Foods
- Ideological and Political Education
- Antimicrobial Resistance in Staphylococcus
- Microbial Metabolic Engineering and Bioproduction
- Vibrio bacteria research studies
- Mine drainage and remediation techniques
- Bipolar Disorder and Treatment
- Bacterial biofilms and quorum sensing
- RNA and protein synthesis mechanisms
- Helicobacter pylori-related gastroenterology studies
- Biotin and Related Studies
- Enzyme Structure and Function
- Arsenic contamination and mitigation
- Biochemical and Molecular Research
- Competency Development and Evaluation
- Cancer-related gene regulation
- Higher Education Learning Practices
- Biochemical Acid Research Studies
- Heavy metals in environment
McGill University
2013-2025
Douglas College
2025
Douglas Mental Health University Institute
2013-2025
Shandong Agricultural University
2019-2024
Air Force Medical University
2023
South China Agricultural University
2007-2021
University of Illinois Urbana-Champaign
2013-2019
Nanjing University of Science and Technology
2018
Urbana University
2015-2017
Center of Hubei Cooperative Innovation for Emissions Trading System
2017
Triclosan, a very widely used biocide, specifically inhibits fatty acid synthesis by inhibition of enoyl-acyl carrier protein (ACP) reductase. Escherichia coli FabI is the prototypical triclosan-sensitive enoyl-ACP reductase, and E. extremely sensitive to biocide. However, other bacteria are resistant triclosan, because they encode triclosan-resistant reductase isozymes. In contrast, triclosan resistance Pseudomonas aeruginosa PAO1 has been attributed active efflux compound (R. Chuanchuen,...
Ultradian (∼4 hr) rhythms in locomotor activity that do not depend on the master circadian pacemaker suprachiasmatic nucleus have been observed across mammalian species, however, underlying mechanisms driving these are unknown. We show disruption of dopamine transporter gene lengthens period ultradian mice. Period lengthening also results from chemogenetic activation midbrain neurons and psychostimulant treatment, while antipsychotic haloperidol has opposite effect. further reveal striatal...
Infradian mood and sleep-wake rhythms with periods of 48 hours beyond have been observed in patients bipolar disorder (BD), which even persist the absence exogenous timing cues, indicating an endogenous origin. Here, we show that mice exposed to methamphetamine drinking water develop infradian locomotor extend sleep length manic state–associated behaviors support a model for cycling BD. The capacity is abrogated upon genetic disruption dopamine (DA) production DA neurons ventral tegmental...
Abstract Although there is evidence for a circadian regulation of the preovulatory LH surge, contributions individual tissue clocks to this process remain unclear. We studied female mice deficient in Bmal1 gene (Bmal1−/−), which essential clock function, and found that they lack proestrous surge. However, spontaneous ovulation on day estrus was unaffected these animals. Bmal1−/− females were also FSH which, like GnRH-dependent. In absence or external timing cues, continued cycle constant...
Enoyl-acyl carrier protein (enoyl-ACP) reductase catalyzes the last step of elongation cycle in synthesis bacterial fatty acids. The Enterococcus faecalis genome contains two genes annotated as enoyl-ACP reductases, a FabI-type and FabK-type reductase. We report that expression either proteins restores growth an Escherichia coli fabI temperature-sensitive mutant strain under nonpermissive conditions. In vitro assays demonstrated both support acid are active with substrates all chain lengths....
Significance Lipoic acid is an enzyme cofactor found throughout the biological world that required for key steps in central metabolism. In humans, defective lipoic synthesis results energy production, accumulation of toxic levels certain amino acids, and early death. The different pathways put forth have not been validated by direct analysis postulated reactions, excepting a protein called LIPT1. Unfortunately, activity reported LIPT1 misleading seems to be evolutionary remnant. We report...
The phospholipid acyl chains of Enterococcus faecalis can be derived either by de novo synthesis or incorporation exogenous fatty acids through the acid kinase complex (Fak)-phosphate acyltransferase (PlsX) pathway. Exogenous suppress transcriptional repressor FabT, loss which eliminated regulation biosynthesis and resulted in decreased unsaturated acids. Purified FabT bound to promoters several genes that contain a specific palindromic sequence binding was enhanced acylated derivatives...
The development of zinc finger nuclease (ZFN) technology has enabled the genetic engineering rat genome. ability to manipulate genome great promise augment utility rats for biological and pharmacological studies. A Wistar Hannover model lacking multidrug resistance protein Mdr1a P-glycoprotein (P-gp) was generated using a Mdr1a-specific ZFN. completely absent in tissues, including brain small intestine, knockout rat. Pharmacokinetic studies with P-gp substrates loperamide, indinavir,...
Abstract In most bacteria the last step in synthesis of pimelate moiety biotin is cleavage ester bond pimeloyl-acyl carrier protein (ACP) methyl ester. The paradigm enzyme Escherichia coli BioH which together with BioC methyltransferase allows by a modified fatty acid biosynthetic pathway. Analyses extant bacterial genomes showed that bioH absent from many bioC- containing and replaced other genes. Helicobacter pylori lacks gene encoding homologue known pimeloyl-ACP enzymes suggesting it...
ABSTRACT Citrobacter rodentium is a murine intestinal pathogen used as model for the foodborne human pathogens enterohemorrhagic Escherichia coli and enteropathogenic E. . During infection, these use two-component signal transduction systems to detect adapt changing environmental conditions. In , CpxRA system responds envelope stress by modulating expression of myriad genes. Quantitative real-time PCR showed that cpxRA was expressed in colon C57BL/6J mice infected with C. To determine...
ABSTRACT Ubiquitin‐specific peptidase 2 (USP2) is a deubiquitinase (DUB) with diversity of functions in physiology. One these the regulation circadian rhythms, which are physiological rhythms period ~24 h. Previous studies have indicated role for USP2 photic entrainment, process by clocks synchronize to environmental light cues. Here, we investigated implication this process, using Usp2 knockout (KO) mice. Using different treatments and running wheel recordings, established that controls...
Abstract Background The original anaerobic unsaturated fatty acid biosynthesis pathway proposed by Goldfine and Bloch was based on in vivo labeling studies Clostridium butyricum ATCC 6015 (now C. beijerinckii ) but to date no dedicated biosynthetic enzyme has been identified Clostridia. acetobutylicium synthesizes the same species of acids as E. coli , lacks all known synthetic genes other bacteria. A possible explanation that two enzymes saturated synthesis FabZ FabF might also function arm...
Acyl carrier proteins (ACPs) play essential roles in the synthesis of fatty acids and transfer long acyl chains into complex lipids. The Enterococcus faecalis genome contains two annotated acp genes, called acpA acpB AcpA is encoded within acid (fab) operon appears essential. In contrast, AcpB an atypical ACP, having only 30% residue identity with AcpA, not Deletion has no effect on E. growth or de novo media lacking acids. However, unlike wild-type strain, where oleic resulted almost...
The renal outer medullary potassium channel (ROMK, KCNJ1) mediates recycling and facilitates sodium reabsorption through the Na(+)/K(+)/2Cl(-) cotransporter in loop of Henle secretion at cortical collecting duct. Human genetic studies indicate that ROMK homozygous loss-of-function mutations cause type II Bartter syndrome, featuring polyuria, salt wasting, hypotension; humans heterozygous for identified Framingham Heart Study have reduced blood pressure. null mice recapitulate many features...
The PhoPQ two-component system of the intracellular pathogen Salmonella enterica senses and controls resistance to alpha-helical antimicrobial peptides (AMPs) by regulating covalent modifications lipid A. A homologue phoPQ operon was found in genome murine enteric extracellular pathogen, Citrobacter rodentium. Here we report that C. rodentium apparently unable mediate activation target genes presence AMPs. However, these AMPs activated expressed a S. entericaDeltaphoPQ mutant. Analysis outer...
Lipoic acid is synthesized by a remarkably atypical pathway in which the cofactor assembled on its cognate proteins. An octanoyl moiety diverted from fatty synthesis covalently attached to acceptor protein, and sulfur insertion at carbons 6 8 of form lipoyl cofactor. Covalent attachment this required for function several central metabolism enzymes, including glycine cleavage H protein (GcvH). In Bacillus subtilis, GcvH sole substrate lipoate assembly. Hence lipoic acid-requiring 2-oxoacid...
FabG is the only known enzyme that catalyzes reduction of 3-ketoacyl-ACP intermediates bacterial fatty acid synthetic pathways. However, there are two Ralstonia solanacearum genes, RSc1052 (fabG1) and RSp0359 (fabG2), annotated as encoding putative reductases. Both homologues possess conserved catalytic triad N-terminal cofactor binding sequence short chain dehydrogenase/reductase (SDR) family. Thus, it seems reasonable to hypothesize RsfabG1 RsfabG2 both encode functional reductases play...