A5016228657- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Neurotransmitter Receptor Influence on Behavior
- Neurological disorders and treatments
- Parkinson's Disease Mechanisms and Treatments
- Receptor Mechanisms and Signaling
- Photoreceptor and optogenetics research
- Neuroscience and Neural Engineering
- Neuroinflammation and Neurodegeneration Mechanisms
- Cardiac Arrhythmias and Treatments
- Congenital heart defects research
- Nerve injury and regeneration
- Cellular transport and secretion
- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced Electron Microscopy Techniques and Applications
- Medicinal Plants and Bioactive Compounds
- Autophagy in Disease and Therapy
- Genetic Neurodegenerative Diseases
- Biochemical Analysis and Sensing Techniques
- Regulation of Appetite and Obesity
- Advancements in Transdermal Drug Delivery
- Mast cells and histamine
- Advanced Neuroimaging Techniques and Applications
- Neurological diseases and metabolism
- Nuclear Receptors and Signaling
Université de Bordeaux
2024
Centre National de la Recherche Scientifique
2024
Institut Interdisciplinaire de Neuroscience
2024
Université de Montréal
2013-2023
Abstract In Parkinson’s disease (PD), motor dysfunctions only become apparent after extensive loss of DA innervation. This resilience has been hypothesized to be due the ability many behaviors sustained through a diffuse basal tone DA; but experimental evidence for this is limited. Here we show that conditional deletion calcium sensor synaptotagmin-1 (Syt1) in neurons (Syt1 cKO mice) abrogates most activity-dependent axonal release striatum and mesencephalon, leaving somatodendritic (STD)...
Current electrophysiology and electrochemistry techniques have provided unprecedented understanding of neuronal activity. However, these are suited to a small, albeit important, panel neurotransmitters such as glutamate, GABA dopamine, constitute only subset the broader range involved in brain chemistry. Surface-enhanced Raman scattering (SERS) provides unique opportunity detect close proximity neurons. Dynamic SERS (D-SERS) nanosensors based on patch-clamp-like nanopipettes decorated with...
A nanothin block copolymer (BCP) brush-layer film adsorbed on glass nanofibers is shown to address the long-standing challenge of forming a template for deposition dense and well-dispersed nanoparticles highly curved surfaces, allowing development an improved nanosensor neurotransmitters. We employed polystyrene- block-poly(4-vinylpyridine) BCP plasmonic gold (AuNPs) 52 nm in diameter fabrication pulled fibers with diameters down 200 nm. The method simple, using only solution processes...
ABSTRACT Dopamine (DA) is a key regulator of circuits controlling movement and motivation. A. subset midbrain DA neurons has been shown to express the vesicular glutamate transporter (VGLUT)2, underlying their capacity for release. Glutamate release found mainly by ventral tegmental area (VTA) can be detected at terminals contacting ventral, but not dorsal, striatal neurons, suggesting possibility that target‐derived signals regulate neurotransmitter phenotype neurons. Whether released from...
Chemical neurotransmission typically occurs through synapses. Previous ultrastructural examinations of monoamine neuron axon terminals often failed to identify a pre- and postsynaptic coupling, leading the concept "volume" transmission. Whether this results from intrinsic properties these neurons remains undefined. We find that dopaminergic in vitro establish distinctive axonal arbor compared glutamatergic or GABAergic both size propensity avoid direct contact with target neurons. While most...
Neuroligins (NLGNs) are important cell adhesion molecules mediating trans-synaptic contacts between neurons. However, the high-yield biochemical isolation and visualization of endogenous NLGNs is hampered by lack efficient antibodies. Thus, to reveal their subcellular distribution, binding partners, synaptic function, were extensively manipulated using knock-down, knock-out, or overexpression approaches, leading controversial results. As an alternative manipulation NLGN expression level, we...
Dopamine (DA) neurons can release DA not just from axon terminals, but also their somatodendritic (STD) compartment through a mechanism that is still incompletely understood. Using voltammetry in mouse mesencephalic brain slices, we find STD has low capacity and shows calcium sensitivity comparable to of axonal release. We the molecular differs with regard implication synaptotagmin (Syt) sensors. While individual constitutive knockout Syt4 or Syt7 sufficient reduce release, removal both...
Midbrain dopamine (DA) neurons are key regulators of basal ganglia functions. The axonal domain these is highly complex, with a large subset non-synaptic release sites and smaller synaptic terminals from which in addition to DA, glutamate or GABA also released. molecular mechanisms regulating the connectivity DA their neurochemical identity unknown. An emerging literature suggests that neuroligins, trans-synaptic cell adhesion molecules, regulate both neuron neurotransmission. However,...
Abstract In Parkinson’s disease (PD), motor dysfunctions only become apparent after extensive loss of DA innervation. This resilience has been hypothesized to be due the ability many behaviors sustained through a diffuse basal tone DA; but experimental evidence for this is limited. Here we show that conditional deletion calcium sensor synaptotagmin-1 (Syt1) in neurons (Syt1 cKO mice) abrogates most activity-dependent axonal release striatum and mesencephalon, leaving somatodendritic (STD)...
ORIGINAL RESEARCH article Front. Syst. Neurosci., 03 October 2013 | https://doi.org/10.3389/fnsys.2013.00057
Dopamine (DA) neurons of the substantia nigra pars compacta (SNc) are uniquely vulnerable to neurodegeneration in Parkinson's disease (PD). We hypothesize that their large axonal arbor is a key factor underlying vulnerability, due increased bioenergetic, proteostatic and oxidative stress. In keeping with this model, other DAergic populations smaller arbors mostly spared during course PD more resistant experimental lesions animal models. Aiming improve mouse models, we examined if neonatal...
ABSTRACT Chemical neurotransmission in the brain typically occurs through synapses, which are structurally and functionally defined as sites of close apposition between an axon terminal a postsynaptic domain. Ultrastructural examinations terminals established by monoamine neurons often failed to identify similar tight pre- coupling, giving rise concept “diffuse” or “volume” transmission. Whether this results from intrinsic properties such modulatory remains undefined. Using efficient...
Abstract Parkinson’s disease (PD) is a neurodegenerative disorder linked to the loss of dopaminergic neurons (DN) in substantia nigra. Although mechanisms triggering DN are unclear, mitochondrial dysfunction and inflammation viewed as playing key role. PINK1 Parkin major regulators mitophagy failure this pathway DNs hypothesized enhanced oxidative stress cause cell death. However, we showed that play also role adaptive immunity by repressing antigen presentation (MitAP) (Matheoud et al.,...
ABSTRACT Dopamine (DA) neurons can release DA not just from axon terminals, but also their somatodendritic (STD) compartment thought a mechanism that is still incompletely understood. Using voltammetry in mouse mesencephalic brain slices, we find STD has low capacity, stable response to electrical optogenetic train pulses and shows calcium sensitivity comparable of axonal release. It strikingly more resilient compared 6‐ hydroxydopamine model Parkinson’s disease plasticity. We the molecular...
Summary Midbrain dopamine (DA) neurons are key regulators of basal ganglia functions. The axonal domain these is highly complex, with a large subset non-synaptic release sites and smaller synaptic terminals from which glutamate or GABA released. molecular mechanisms regulating the connectivity DA their neurochemical identity unknown. Here we tested hypothesis that trans-synaptic cell adhesion molecules neurexins (Nrxns) regulate neuron neurotransmission. Conditional deletion all Nrxns in...
Abstract Neuroligins (NLGNs) are important cell adhesion molecules mediating trans-synaptic contacts between neurons. However, the high-yield biochemical isolation and visualization of endogenous NLGNs have been hampered by lack efficient antibodies to these proteins. Thus, reveal their sub-cellular distribution, binding partners, synaptic function, extensively manipulated using knock-down, knock-out, or over-expression approaches, overall leading controversial results. As an alternative...
In 1992, a landmark paper by Johnson and North published in the Journal of Physiology entitled "Two types neurone rat ventral tegmental area (VTA) their synaptic inputs" (Johnson & North, 1992) provided first glimpse diversity neurons this part brain. The VTA was initially better known for presence dopamine (DA)-containing projecting to striatum its key role motivated behaviors drug addiction. work revealed existence two distinct categories that they named "principal cells" "secondary...