A5099644372- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Advanced Electron Microscopy Techniques and Applications
Institut Interdisciplinaire de Neuroscience
2024-2025
Université de Bordeaux
2025
Centre National de la Recherche Scientifique
2024
Neuroligins (NLGNs) are important cell adhesion molecules mediating trans-synaptic contacts between neurons. However, the high-yield biochemical isolation and visualization of endogenous NLGNs is hampered by lack efficient antibodies. Thus, to reveal their subcellular distribution, binding partners, synaptic function, were extensively manipulated using knock-down, knock-out, or overexpression approaches, leading controversial results. As an alternative manipulation NLGN expression level, we...
Super-resolution imaging provides unprecedented visualization of sub-cellular structures, but the two main techniques used, single-molecule localization microscopy (SMLM) and stimulated emission depletion (STED), are not easily reconciled. We present a protocol to super-impose nanoscale protein distribution reconstructed with SMLM morphology obtained in STED. describe steps for tracking cells on etched coverslips registering images from different microscopes 30-nm accuracy. In this protocol,...