A5007362503- Neuroscience and Neuropharmacology Research
- Advanced Fluorescence Microscopy Techniques
- Cellular transport and secretion
- Receptor Mechanisms and Signaling
- Neurological Disorders and Treatments
- Medicinal Plants and Bioactive Compounds
- Advanced Electron Microscopy Techniques and Applications
- Cell Image Analysis Techniques
Institut Interdisciplinaire de Neuroscience
2022-2025
Université de Bordeaux
2022-2025
Centre National de la Recherche Scientifique
2022-2024
Neuroligins (NLGNs) are important cell adhesion molecules mediating trans-synaptic contacts between neurons. However, the high-yield biochemical isolation and visualization of endogenous NLGNs is hampered by lack efficient antibodies. Thus, to reveal their subcellular distribution, binding partners, synaptic function, were extensively manipulated using knock-down, knock-out, or overexpression approaches, leading controversial results. As an alternative manipulation NLGN expression level, we...
Introduction The synaptic adhesion molecule neuroligin-1 (NLGN1) is involved in the differentiation of excitatory synapses, but precise underlying molecular mechanisms are still debated. Here, we explored role NLGN1 tyrosine phosphorylation this process, focusing on a subset receptor kinases (RTKs), namely FGFR1 and Trks, that were previously described to phosphorylate at unique intracellular residue (Y782). Methods We used pharmacological inhibitors genetic manipulation those RTKs...
Neuroligins (NLGNs) form a family of cell adhesion molecules implicated in synapse development, but the mechanisms that retain these proteins at synapses are still incompletely understood. Recent studies indicate surface-associated NLGN1 is diffusionally trapped synapses, where it interacts with quasi-static scaffolding elements post-synaptic density. Whereas single molecule tracking reveals rapid diffusion and transient immobilization within seconds, fluorescence recovery after...
Abstract Neuroligins (NLGNs) are important cell adhesion molecules mediating trans-synaptic contacts between neurons. However, the high-yield biochemical isolation and visualization of endogenous NLGNs have been hampered by lack efficient antibodies to these proteins. Thus, reveal their sub-cellular distribution, binding partners, synaptic function, extensively manipulated using knock-down, knock-out, or over-expression approaches, overall leading controversial results. As an alternative...