A5045230482- Heat shock proteins research
- Epigenetics and DNA Methylation
- Genetics, Aging, and Longevity in Model Organisms
- Acute Myeloid Leukemia Research
- Immune Response and Inflammation
- Histone Deacetylase Inhibitors Research
- Acute Lymphoblastic Leukemia research
- Fatty Acid Research and Health
- Endoplasmic Reticulum Stress and Disease
- RNA Research and Splicing
- Developmental Biology and Gene Regulation
- Cancer-related gene regulation
- Immunotherapy and Immune Responses
- Spaceflight effects on biology
- Nutrition, Genetics, and Disease
- Genomics and Chromatin Dynamics
- Immune cells in cancer
- TGF-β signaling in diseases
- Eicosanoids and Hypertension Pharmacology
- RNA modifications and cancer
- Animal testing and alternatives
- Cancer, Hypoxia, and Metabolism
- S100 Proteins and Annexins
- Erythropoietin and Anemia Treatment
- Cancer Research and Treatments
Marshall University
2011-2024
Karolinska Institutet
2022
Thomas Jefferson University
2002-2006
Sidney Kimmel Cancer Center
2002-2005
University of Kansas
1997-2000
Evolutionary biology is currently experiencing an emergence of several research topics that transcend the boundaries Modern Synthesis, which was last major conceptual integration in evolutionary [1]. The Synthesis used concepts population genetics to integrate Mendelian with evolution by natural selection [2]. Pigliucci [3, and citations within] identified areas innovation Synthesis: epigenetics, evolvability, phenotypic plasticity, on adaptive landscapes, developmental biology, systems...
Cancer stem cells (CSCs) are considered a subset of the bulk tumor responsible for initiating and maintaining disease. Several surface cellular markers have been recently used to identify CSCs. Among those is CD133, which expressed by hematopoietic progenitor as well embryonic various cancers. We isolated cultured CD133 positive [CD133(+)] from cancer cell lines using NASA developed Hydrodynamic Focusing Bioreactor (HFB) (Celdyne, Houston, TX). For comparison, another bioreactor, rotary...
Ovarian cancer is one of the leading causes death for women with worldwide. Energy requirements tumor growth in epithelial high-grade serous ovarian (HGSOC) are fulfilled by a combination aerobic glycolysis and oxidative phosphorylation (OXPHOS). Although reduced OXPHOS activity has emerged as significant contributors to aggressiveness chemoresistance, up-regulation mitochondrial antioxidant capacity required matrix detachment colonization into peritoneal cavity form malignant ascites HGSOC...
Abstract Background Omega 3 fatty acids have been found to inhibit proliferation, induce apoptosis, and promote differentiation in various cell types. The processes of survival, expansion, are key importance the regulation hematopoiesis. We investigated role omega controlling frequency myeloid progenitor cells bone marrow mice. Increased blocked characteristics hematopoietic disorders lineage, such as myeloproliferative diseases leukemias. Results that increasing proportion relative 6 diet...
Pseudomonas aeruginosa is an opportunistic pathogen that causes serious respiratory disease in the immune-compromised host. Using aerosol infection model, 11 inbred mouse strains (129/Sv, A/J, BALB/c, C3H/HeN, C57BL/6, DBA/2, FVB, B10.D2/oSnJ, B10.D2/nSnJ, AKR/J and SWR/J) were tested for increased susceptibility to P. lung colonization. DBA/2 was only strain had bacterial counts within 6 h post-infection. This deficiency incited a marked inflammatory response with reduced clearance...
Many melanoma cells are resistant to the anti-proliferative effect of all trans retinoic acid (ATRA). Retinoic Acid Receptor-beta2 (RAR-beta2) mediates ATRA growth inhibition. We found a correlation between activity and expression RAR-beta2. There was not strict DNA methylation RAR-beta gene its expression. no difference in global RARbeta specific nucleosome repeat length (NRL) melanocytes or control treated cells. Pan-acetylation H3 H4 within promoter higher expressing All treatment...
Understanding the role of iron in ethanol-derived hepatic stress could help elucidate efficacy dietary or clinical interventions designed to minimize liver damage from chronic alcohol consumption. We hypothesized that normal levels are involved and reduced intake would lower caused by ethanol. used a pair-fed mouse model utilizing basal Lieber-DeCarli liquid diets for 22 weeks test this hypothesis. In our model, ethanol exposure led mild possibly characteristic early-stage alcoholic disease,...
Abstract The effects of the polyunsaturated omega‐3 (n‐3) and omega‐6 (n‐6) fatty acids (FA) on hematopoiesis are complex in that both FA forms processed into leukotrienes, eicosanoids, prostaglandins, which can have independent effects. These antagonistic n‐6 prostaglandins tend to be pro‐proliferative pro‐inflammatory, while n‐3 opposite. We previously shown diets high reduce size middle later stage myeloid progenitor compartment FVB X sv129 F 1 hybrid mice. To assay earlier stages...
Despite advances in cancer treatment, drug resistance and metastasis continue to contribute treatment failure. Since are features that often occur toward the late stages disease after withstanding numerous selective pressures, they may rely on a shared adaptive mechanism order persist. The heat shock response is one of most well conserved responses cellular stress found nature. A major player HSP90, with some studies suggesting it can facilitate molecular evolution cancer. Non-small cell...
Cancer cells exist in a state of Darwinian selection using mechanisms that produce changes gene expression through genetic and epigenetic alteration to facilitate their survival. Cellular plasticity, or the ability alter cellular phenotype, can assist survival premalignant as they progress full malignancy by providing another mechanism adaptation. The connection between stress progression cancer has been established, although details have yet be fully elucidated. molecular chaperone HSP90 is...
We have previously demonstrated that the Na-K-ATPase signaling-mediated oxidant amplification loop contributes to experimental uremic cardiomyopathy and anemia induced by 5/6th partial nephrectomy (PNx). This process can be ameliorated systemic administration of peptide pNaKtide, which was designed block this loop. The present study PNx-induced is characterized marked decreases in red blood cell (RBC) survival as assessed biotinylated RBC clearance eryptosis annexin V binding. No significant...
The emerging importance of epigenetic gene regulation in cancer progression necessitates not only our understanding which genes are potential targets but also what mechanisms employed targeting those genes. Understanding the that promote evolution normal genome and epigenome is central to how cells adapt chemotherapy. Our previous investigations have shown heat shock protein 90 (HSP90) has a critical role through histone acetylation phenotypic plasticity. We recently extended these results...
We have isolated a Meis1a transgenic mouse line exhibiting recessive male-specific lethality and non-alcoholic fatty liver disease (NAFLD), which coincides with pubescence is androgen-dependent. The phenotype due to disruption of an endogenous locus, since other lines do not exhibit these phenotypes. Necropsy analysis revealed hepatic microvesicular steatosis in pubescent male homozygous mice, absent females. transgene insertion site was localized chromosome 1 further refined by cloning the...