- Neurobiology and Insect Physiology Research
- Plant Pathogens and Fungal Diseases
- Aquaculture Nutrition and Growth
- Plant Disease Resistance and Genetics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Microbial Metabolic Engineering and Bioproduction
- Invertebrate Immune Response Mechanisms
- Crustacean biology and ecology
- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Reproductive biology and impacts on aquatic species
- Algal biology and biofuel production
- Animal Behavior and Reproduction
- Powdery Mildew Fungal Diseases
- Lipid metabolism and biosynthesis
- Physiological and biochemical adaptations
- Cholinesterase and Neurodegenerative Diseases
- Fungal Plant Pathogen Control
- Enzyme Catalysis and Immobilization
- Insect Resistance and Genetics
- Computational Drug Discovery Methods
- Water Quality Monitoring Technologies
- Hypothalamic control of reproductive hormones
- Plant Disease Management Techniques
- Plant Pathogens and Resistance
Zhongkai University of Agriculture and Engineering
2020-2025
Sun Yat-sen University
2025
Institute of Vegetables and Flowers
2010-2024
Chinese Academy of Agricultural Sciences
2010-2024
Ningbo University
2014-2024
Jilin Agricultural University
2024
South China Agricultural University
2013-2021
University of Saskatchewan
2017-2020
Zero to Three
2020
Institute of Crop Sciences
2008-2013
Abstract Rice false smut caused by Ustilaginoidea virens is an important constraint affecting rice yield and quality in Asia. In China, especially severe the japonica rice‐growing areas North China. Nothing known neither about diversity of pathogen this region nor characteristic its local population. study, 110 U. isolates sampled from Liaoning Beijing China were analysed using amplified fragment length polymorphism (AFLP) markers to primarily understand genetic population characteristics....
False smut, caused by U stilaginoidea virens , is a rice disease of increasing importance worldwide, with no source high‐level resistance in the existing germplasm. To facilitate breeding varieties good levels field to false quantitative loci ( QRL ) were identified using 213 introgression lines IL s) from cross between T eqing (recipient) and L emont (donor) evaluated natural infection at two hotspots smut northeast C hina. Ten affecting percentages diseased hills, panicles spikelets...
Methyl farnesoate (MF) is a sesquiterpene compound and the crustacean homolog of insect juvenile hormones. MF has multiple physiological functions involving regulation molting, reproduction, metamorphogenesis, behavior, osmoregulation. In this study, hemolymph levels during ovarian development Portunus trituberculatus were measured by gas chromatography-mass spectrometry (GC-MS). The results showed that level in P. was low stages I II, increased considerably stage III, remained high IV....
It has been shown in recent studies that the crustacean female sex hormone (CFSH) plays a crucial role development of secondary sexual characteristics Decapoda crustaceans. However, research on function CFSH eyestalk-AG-testicular endocrine axis inadequate. We cloned and identified homolog CFSH, PtCFSH, this study. RT-PCR showed PtCFSH was mainly expressed eyestalk. A long-term injection dsPtCFSH recombinant (rPtCFSH) vivo opposite effects spermatogenesis-related gene expression histological...
Cucumber angular leaf spot caused by Pseudomonas syringae pv. lachrymans (Psl) is an important and destructive disease worldwide, no effective technique has been developed for the control of pathogen. Detection infection or latent in cucumber plants critical to evaluate progress strengthening management avoid a serious epidemic fields. In this paper, we rapid sensitive method detection Psl using isothermal known as loop‐mediated amplification (LAMP). A set six primers was designed amplify...
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CYP15, which encodes a microsomal cytochrome P450 enzyme, could be involved in juvenile hormone biosynthesis insects. In this study, full-length cDNA of CYP15 was cloned from the swimming crab Portunus trituberculatus. This PtCYP15 amino acid sequence contains six conserved domains, is typical feature family. Phylogenetic tree analysis results showed that clusters single branch crustacean species, suggesting may more widely present crustaceans. The mRNA has broad pattern tissue expression P....
Thraustochytrium sp. strain ATCC 26185 accumulates a high level of docosahexaenoic acid (DHA), nutritionally important ω-3 very-long-chain polyunsaturated fatty (VLCPUFA) synthesized primarily by (PUFA) synthase, type I polyketide synthase-like megaenzyme. The PUFA synthase in this species comprises three large subunits, each with multiple catalytic domains. It was hypothesized that among these domains, ketoacylsynthase (KS) domains might be critical for catalyzing the condensation specific...
Two full-length cDNA (Pt-CHH1 and Pt-CHH2) sequences encoding crustacean hyperglycemic hormone (CHH) were cloned from tissues of the swimming crab (Portunus trituberculatus) using RACE. Pt-CHH1 was eyestalk, whereas Pt-CHH2 thoracic ganglia. Sequence structure analyses suggest that they may be generated alternative splicing. Tissue distribution showed transcript only detected in while observed several extra-eyestalk tissues. The levels during molting ovarian development determined qPCR. In...
The insulin-like androgenic gland hormone (IAG) is mainly produced in the (AG) of male crustaceans, and a crucial regulator sexual differentiation. In current study, full-length cDNA IAG swimming crab, Portunus trituberculatus (Pt-IAG), was cloned characterized. Similar to other reported IAGs, deduced amino acid sequence Pt-IAG consist signal peptide, B chain, C peptide A containing 6 conserved cysteines that form two inter-chain disulfide bonds one intra-B chain bond. Tissue distribution...
Corazonin (Crz) is a neuropeptide that widely distributed in insects and crustaceans. The Crz proposed to have pleiotropic functions insects, but its physiological roles crustaceans are poorly understood. In the present study, putative receptor ( CrzR ) were identified from swimming crab, Portunus trituberculatus , their interaction was validated using Dual-Luciferase reporter assay system. Tissue distribution analysis showed PtCrz mainly derived center nerve system, while highly expressed...
The present study was performed to investigate whether Plasmodiophora brassicae can be disseminated by livestock manure. A quantitative PCR ( qPCR ) assay developed and used detect quantify P. in manure samples from naturally artificially infested chickens pigs. In manure, quantifiable levels of infestation were observed 7 out the 28 samples, ranging 10 3 resting spores per g vast majority (76–91%) viable, as determined a dual fluorescence viability assay. Clubroot symptoms on plants...